天然产物研究与开发 ›› 2022, Vol. 34 ›› Issue (7): 1129-1142.doi: 10.16333/j.1001-6880.2022.7.005

• 研究论文 • 上一篇    下一篇

基于UPLC-Q-TOF-MS的苦水玫瑰醇提物化学成分分析

杜少波1,2,3,陈晓文1,2,3,董志强1,2,3,谢惠春1,2,3*   

  1. 1青海师范大学生命科学学院;2青海省青藏高原药用动植物资源重点实验室;3青藏高原生物资源形成机制与综合利用重点实验室,西宁 810008
  • 出版日期:2022-07-28 发布日期:2022-07-25
  • 基金资助:
    青海省科技厅科技成果转化专项项目(2020-NK-125)

Analysis of chemical components in alcohol extract of Rosa sertata×Rosa rugosa based on UPLC-Q-TOF-MS

DU Shao-bo1,2,3,CHEN Xiao-wen1,2,3,DONG Zhi-qiang1,2,3,XIE Hui-chun1,2,3*   

  1. 1Academy of Life Science,Qinghai Normal University;2Qinghai Province Key Laboratory of Medicinal Animal and Plant Resources of Qinghai-Tibetan Plateau;3Qinghai Province Key Laboratory of Biodiversity Formation Mechanism and Comprehensive Utilization of Qinghai-Tibetan Plateau,Xining 810008,China
  • Online:2022-07-28 Published:2022-07-25

摘要: 为了对苦水玫瑰醇提物的化学成分进行快速分析鉴定,本研究采用超高效液相色谱-四级杆-飞行时间质谱(UPLC-Q-TOF-MS)技术,选用Waters ACQUITY UPLC HSS T3(100 mm×2.1 mm,1.8 μm)色谱柱,流动相为0.1%甲酸水溶液(A)-0.01%甲酸乙腈溶液(B),梯度洗脱。在电喷雾离子源(ESI)负离子条件下,采集MSE数据。根据精确相对分子质量、质谱碎片信息,结合相关文献及对照品比对,共检测出63个主要成分,包括26个黄酮类、11个多酚类(4个酚酸类及7个鞣质类)、7个三萜类、4个苯丙素类、8个有机酸类、1个环烯醚萜类、1个挥发油类化合物和5个未鉴定成分,其中柳穿鱼叶苷、草木樨苷、积雪草酸等28个化合物首次在该属得到鉴定。通过UPLC-Q-TOF-MS方法进一步完善了苦水玫瑰的化学成分,为药效物质基础研究提供了参考依据。

关键词: 超高效液相色谱-四级杆-飞行时间质谱, 苦水玫瑰, 醇提物, 化学成分

Abstract:

In order to quickly analyze and identify the chemical components of the alcohol extract of Rosa sertata×Rosa rugosa,ultra performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) was used in this study,Waters ACQUITY UPLC HSS T3 column (100 mm×2.1 mm,1.8 μm) was used with The mobile phase is 0.1% formic acid aqueous solution (A) and 0.01% formic acid acetonitrile solution (B) and gradient elution.The MSE data were collected under the negative ion condition of electrospray ion source (ESI).Sixty-three main components were detected according to the exact relative molecular mass,mass spectrometry fragmentation information,and related literature and reference materials,including 26 flavonoids,11 polyphenols(4 phenolic acids and 7 tannins),7 triterpenoids,4 phenylpropanoids,8 organic acids,1 iridoid terpene,1 volatile oil and 5 unidentified components.Twenty-eight compounds such as pectolinarin,melilotoside and asiatic acid were identified in this genus for the first time.UPLC-Q-TOF-MS method was used to further improve the chemical constituents of Rosa sertata×Rosa rugosa,which provided a reference basis for the basic study of pharmacodynamic substances.

Key words: UPLC-Q-TOF-MS, Rosa sertata×Rosa rugosa, alcohol extract, chemical composition

中图分类号:  O657.7