天然产物研究与开发 ›› 2022, Vol. 34 ›› Issue (7): 1189-1195.doi: 10.16333/j.1001-6880.2022.7.012

• 开发研究 • 上一篇    下一篇

铁皮石斛软腐病菌终极腐霉生物学特性及其抑菌植物筛选

谭智勇1,2,4,周震宇1*,杨文明3,李亚娟5,卢志宏2   

  1. 1湘南学院,郴州 423000;2铜仁学院,铜仁 554300;3贵州梵天农业科技有限责任公司,铜仁 554400;4湖南梵宇景观规划设计有限责任公司,郴州423000;5铜仁市农业科教信息站,铜仁 554300
  • 出版日期:2022-07-28 发布日期:2022-07-25
  • 基金资助:
    贵州省科技厅科技计划(黔科合支撑[2020]4Y065、[2020]4Y069);贵州省教育厅青年科技人才成长项目(黔教合KY字[2019]175);铜仁学院2021年硕士点及学科建设研究子项目(trxyxwdxm-029)

Biological characteristics of Pythium ultimum causing damping-off disease of Dendrobium officinale and the screening of antifungal plants against it

TAN Zhi-yong1,2,4,ZHOU Zhen-yu1*,YANG Wen-ming3,LI Ya-juan5,LU Zhi-hong2   

  1. 1Xiangnan University,Chenzhou 423000,China;2Tongren University,Tongren 554300,China;3Guizhou Fantian Agricultural Technology Co.,Ltd.,Tongren 554400,China;4Hunan Fanyu Landscape Planning and Design Co.,Ltd.,Chenzhou 423000,China;5Tongren Agricultural Science and Education Information Station,Tongren 554300,China
  • Online:2022-07-28 Published:2022-07-25

摘要:

开展铁皮石斛软腐病菌终极腐霉(Pythium ultimum)生物学特性及其抑菌植物筛选研究,为铁皮石斛软腐病防治奠定理论基础。采用菌丝生长速率法测定不同中药材水提物对铁皮石斛软腐病菌终极腐霉的抑制作用及其生物学特性。终极腐霉在PDA培养基上菌丝平均生长速度为0.22 cm/h,最适宜的温度为30 ℃,最适宜的pH在6~7,最适碳源为葡萄糖、可溶性淀粉,最适氮源为酵母浸膏,光照对菌丝生长影响不显著。当水提物浓度为40 mg干样/mL时,丁香和薄荷的抑制率均分别为100%和96.48%。丁香和薄荷水提物具有防治铁皮石斛软腐病的良好应用潜力。

关键词: 铁皮石斛, 软腐病, 终极腐霉, 抑菌植物

Abstract:

The biological characteristics of Pythium ultimum causing damping-off disease of Dendrobium officinale and the screening of antifungal plants against P. ultimum were studied to lay a theoretical foundation for the prevention and control of damping-off disease of D. officinale. The inhibitory effect and biological characteristics of aqueous extracts of different Chinese herbal medicines on the  P. ultimum were determined by mycelium growth rate method.The average mycelial growth rate of the  P. ultimum on the PDA medium was 0.22 cm/h.The optimum temperature was 30 °C,the optimum pH was 6-7,the optimum carbon source were glucose and soluble starch,and the optimum nitrogen source was yeast extract. The light has no significant impact on the growth of mycelium. When the concentration of aqueous extract was 40 mg DW/mL,the inhibition rates of aqueous extract of Eugenia caryophyllata Thunb. and Mentha haplocalyx Briq. were 100% and 96.48%,respectively. The water extracts of E. caryophyllata and M. haplocalyx have good application potential in the control of Dendrobium officinale damping-off disease.

Key words: Dendrobium officinale, damping-off disease, Pythium ultimum, antifungal plants

中图分类号:  S432.1