天然产物研究与开发 ›› 2022, Vol. 34 ›› Issue (增刊1): 33-38.doi: 10.16333/j.1001-6880.2022.S.005

• 研究论文 • 上一篇    下一篇

基于肥大细胞的浙江蜡梅精油缓解类风湿性关节炎功效研究

李枢航，王文翠，姚   雷*   

  1. 上海交通大学设计学院，上海 200240
  • 出版日期:2022-06-01 发布日期:2022-05-18
  • 基金资助:
    精油成分测定、分析、评价与功效研究横向项目（17H10 0000466）

An study on the anti-rheumatoid arthritis effects of Chimonanthus zhejiangensis essential oil based on human mast cells

LI Shu-hang,WANG Wen-cui,YAO Lei*   

  1. School of Design,Shanghai Jiao Tong University,Shanghai 200240,China
  • Online:2022-06-01 Published:2022-05-18

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摘要： 为探究浙江蜡梅精油对类风湿性关节炎中肥大细胞活化的抑制作用及主要单体的功效性，采用水蒸气蒸馏法提取精油，GC-MS检测精油成分，利用CCK-8法检验精油与主成分对细胞的毒性，使用C48/80刺激肥大细胞活化，通过ELISA法检测精油和主成分对肥大细胞活化的影响。结果表明，浙江蜡梅的主要成分为桉叶油醇；精油及桉叶油醇在低、中、高浓度下均能抑制肥大细胞脱颗粒，使细胞因子释放量显著降低；相同浓度下，精油对肥大细胞脱颗粒及细胞因子的抑制作用优于桉叶油醇。可推测浙江蜡梅精油应具有缓解类风湿性关节炎的效果，其中的主成分桉叶油醇发挥重要作用。

关键词: 精油, 浙江蜡梅, 类风湿性关节炎, 肥大细胞, 桉叶油醇, 脱颗粒

Abstract:

This study is aimed to explore the inhibitory effect of essential oil from Chimonanthus zhejiangensis on mast cell activation in rheumatoid arthritis and the efficacy of its main monomer.The essential oil was extracted by hydrodistillation,and gas chromatography-mass spectrometry (GC-MS) was used to analyze the volatile chemical compositions and determine the content of the main component.The cell toxicity of essential oil and the main component was tested by cell counting kit 8 (CCK-8).LAD2 was stimulated by C48/80.The degranulation and release of cytokines from LAD2 at different concentrations of essential oil and its main component were detected by linked immunosorbent assay (ELISA).The positive drug methotrexate was selected as the control.The results showed that the main component of essential oil from C. zhejiangensis was eucalyptol,and its content was 0.805 g/mL;essential oil and eucalyptol could inhibit mast cell degranulation at low,medium and high concentrations,and significantly reduced the release of cytokines including IL-4,IL-8,TNF-α and VEGF;the inhibitory effect of essential oil was better than that of eucalyptol at the same concentration,which was close to the effect of the positive drug.In a conclusion,both the essential oil from C. zhejiangensis and eucalyptol can inhibit the degranulation and inflammatory reaction of mast cells.It can be inferred that the essential oil from C. zhejiangensis has the effect on relieving rheumatoid arthritis,in which eucalyptol plays an important role as the main component.

Key words: essential oil, Chimonanthus zhejiangensis, rheumatoid arthritis, mast cells, eucalyptol, degranulation

中图分类号:  R965

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