天然产物研究与开发 ›› 2020, Vol. 32 ›› Issue (11): 1896-1903.doi: 10.16333/j.1001-6880.2020.11.013

• 开发研究 • 上一篇    下一篇

基于果蝇模式生物差异基因表达的刺玫果抗衰老作用机理研究

翟春梅,怀雪,孟祥瑛,付敬菊,秦蓁,孟永海*


  

  1. 黑龙江中医药大学,哈尔滨 150040

  • 出版日期:2020-11-28 发布日期:2020-12-07
  • 基金资助:
    哈尔滨市科技创新人才研究专项(2017RAQXJ125);黑龙江中医药大学骨干教师支持计划(yjscx2017060);黑龙江省博士后科研启动金资助项目(LBH-Q15137)

Study on anti-aging mechanism of Rosa davurica Pall. based on differential gene expression in Drosophila model

ZHAI Chun-mei,HUAI Xue,MENG Xiang-ying,FU Jing-ju,QIN Zhen,MENG Yong-hai*#br#

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  1. Heilongjiang University of Chinese Medicine,Harbin 150040,China

  • Online:2020-11-28 Published:2020-12-07

摘要: 本研究以果蝇为模式生物,以果蝇行为学、基因测序技术为研究方法,考察刺玫果抗衰老活性以及引起的抗衰老相关基因表达的变化,阐明刺玫果抗衰老的作用机制。研究结果发现:给与刺玫果醇提物喂养的果蝇与空白对照组果蝇相比,共发现128个差异基因,其中包括53个上调,75个下调。刺玫果可上调HSP、HSP70、HSP73等长寿基因和IMP2基因来提高了果蝇应激能力,可下调ACTB-G1、Actin、HIPPO等基因调控果蝇吞噬体内吞噬作用、光传导和HIPPO等信号通路,提供了正常喂养和刺玫果醇提物喂养的果蝇生长过程中的基因表达谱和差异表达信息。本研究揭示了刺玫果在抑制果蝇衰老过程中差异关键基因表达特征,为刺玫果等野生果类资源的再利用和深度产业化开发提供实验依据。


关键词: 刺玫果, 果蝇, 转录组学, 差异基因, 机理

Abstract:

In this study,to elucidate the anti-aging mechanism caused by Rosa davurica Pall.,the examination of ethology and gene sequencing technology were used as research methods to investigate the anti-aging activity and the changes of anti-aging-related genes by Drosophila as a model.The results found that a total of 128 differential genes were found in Drosophila melanogaster compared with blank group,including 53 up-regulated and 75 down-regulated.Ethanol extract of Rosa davurica can up-regulate longevity genes such as HSP,HSP70,HSP73 and IMP2 genes to improve the stress ability of Drosophila,and can down-regulate genes such as ACTB-G1,Actin,HIPPO and so on to regulate phagocytosis,light transmission and HIPPO signals.These pathways provide the gene expression profile and differential expression information during the growth of Drosophila affected by Rosa davurica.And it reveals the key gene expression characteristics during the inhibition of Drosophila melanogaster aging.This study can provide experimental basis for the reuse and deep industrialization development of Rosa davurica resources.

中图分类号:  R285