To study the effect of polysaccharide from Dicliptera chinensis (DCP) on LIN28A/NF-κB signaling pathway in liver fibrosis.Sixty male SD rats were randomly divided into normal group,model group,colchicine group and DCP low,medium and high dose (50,100 and 200 mg/kg) groups with 10 rats in each group.The liver fibrosis rat model was established by intraperitoneal injection of 40% CCl₄ olive oil solution (1 mL/kg) twice a week.The normal group was injected with olive oil at the same dose.The rats in the colchicine group and the DCP low,medium and high dose group were given the corresponding drugs every day according to their body weight.The rats in the normal group and the model group were given distilled water according to the same calculation method.The modeling and treatment process lasted 6 weeks.At the end point of the experiment,HE staining and Masson staining were used to observe and evaluate the degree of liver tissue lesions in rats The levels of HA,LN,PC III,IV-C in serum and inflammatory factors (IL-1β,IL-6 and TNF-α) in liver tissue were detected by ELISA.The mRNA and protein expression levels of α-SMA,TGF-β1,LIN28A,NF-κBp65,MMP9,COX-2 and iNOS in rat liver tissue were detected and analyzed by PCR and Western blot.The results showed that the contents of HA,LN,PC III and IV-C in rat serum in colchicine and various doses of DCP groups,and the effect was obvious in DCP high dose group.The pathological results showed that the degree of liver lesions in DCP group and colchicine group were significantly relieved compared with that in model group.The expression of α-SMA,TGF-β1,LIN28A,NF-κBp65,MMP9,COX-2 and iNOS in liver tissue was decreased in different degrees.To sum up,DCP can obviously improve HF in rats,and its mechanism is related to the inhibition of NF-κB signaling pathway by DCP.