天然产物研究与开发 ›› 2019, Vol. 31 ›› Issue (增刊1): 5-9.doi: 10.16333/j.1001-6880.2019.S.002

• 研究论文 • 上一篇    下一篇

预知子提取物在卷烟中的应用及化学成分研究

丁为1,陈小敏2,李剑政2,郑凯清2,林谦2,王晶2*   

  1. 1四川中烟工业有限责任公司,成都 610066;2深圳波顿香料有限公司研发中心,深圳 518051
  • 出版日期:2019-08-06 发布日期:2019-08-06

Application of Akebia trifoliata extract in cigarette and its chemical constituents

DING Wei1,CHEN Xiao-min2,LI Jian-zheng2,ZHENG Kai-qing2,LIN Qian2,WANG Jing2*   

  1. 1China Tobacco Sichuan Industrial Co.,Ltd.,Chengdu 610066,China; 2Shenzhen Boton Flavor and Fragrances Co.,Ltd.,Shenzhen 518051,China
  • Online:2019-08-06 Published:2019-08-06

摘要: 为开发新型天然烟用香料,以预知子为原料靶向分离致香物质,并应用于卷烟加香过程中。试验考察了不同柱填料、乙醇浓度对分离样品卷烟加香效果的影响,确定应用于卷烟加香的预知子提取物的分离纯化工艺条件,并对最佳工艺制备得到的预知子提取物化学物质基础进行研究。通过高效制备色谱、凝胶柱等分离手段,从该提取物中分离纯化出6个化合物,利用ESI-MS、1H NMR、13C NMR等波谱方法结合理化性质确定化学结构,它们分别为:异香兰酸(1)、 neochlorogenic acid methyl ester(2)、 3,5-二咖啡酰奎尼酸甲酯(3)、osmanthuside E(4)、木通苯乙醇苷 B(5) 和2-(4-hydroxyphenyl)ethyl-(6-O-feruloyl)-β-D-glucopyra-noside(6)。结果表明:使用AB-8作填料,50%乙醇作洗脱剂分离精制的样品效果最为显著,能够提升香气质,生津效果明显,改善刺激,柔和烟气。

关键词: 预知子, 卷烟, 加香, 化学成分

Abstract: In order to develop a new type of natural tobacco spice,Akebia trifoliata was used as raw material to separate the flavoring substances,and it is applied to tobacco processing.The effects of different column fillers and ethanol concentration on the aromatic addition of cigarette were investigated.The separation and purification conditions of the Akebia trifoliata extracts applied to the cigarette flavoring were determined,and the chemical basis of the best preparation was studied Six compounds were isolated and purified from the extract by means of high-performance preparation chromatography and gel column,and the chemical structure was determined by combining physical and chemical properties using ESI-MS,1H NMR,13C NMR and other spectral methods.They are:Isovanillin acid (1),neochlorogenic acid methyl ester (2),3,5-dicoffee methyl acyl quinone (3),osmanthuside E (4),xyphenyl glycoside B(5) and 2-(4-hydroxyphenyl)ethyl-(6-O-feruloyl)-β-D-glucopyra-noside (6).The results showed that the use of AB-8 as a filler and 50% ethanol as the eluent to separate and refine the sample was the most effective,which could significantly enhance the fragrance,enhance the sweetness,improve the stimulation,soft smoke.

Key words: Akebia trifoliata, cigarette, flavoring, chemical components

中图分类号: 

R284.2