天然产物研究与开发 ›› 2021, Vol. 33 ›› Issue (3): 362-372.doi: 10.16333/j.1001-6880.2021.3.002

• 研究论文 • 上一篇    下一篇

基于GC-MS的荆芥和荆芥穗饮片挥发油中化学成分特征分析

程梦娟1,2,耿晓桐3,龚海燕1,2*,谢彩侠1,2*,张娟1,2,陈志红1,2,杜宇1,雷敬卫1,2


  

  1. 1河南中医药大学;2河南省中药质量控制与评价工程技术研究中心,郑州 450046;3信阳农林学院,信阳 464000

  • 出版日期:2021-03-28 发布日期:2021-04-01
  • 基金资助:
    国家重点研发计划(2017YFC1700705);河南省高等学校重点科研项目(20A360016)

Analysis of chemical composition characteristics of volatile oil in Schizonepetae Herba and Schizonepetae Spica based on GC-MS

CHENG Meng-juan1,2,GENG Xiao-tong3,GONG Hai-yan1,2*,XIE Cai-xia1,2*,ZHANG Juan1,2,CHEN Zhi-hong1,2,DU Yu1,LEI Jing-wei1,2   

  1. 1Henan University of Chinese Medicine; 2Henan Province Traditional Chinese Medicine Quality Control and Evaluation Engineering Technology Research Center,Zhengzhou 450046,China; 3Xinyang Agriculture and Forestry University,Xinyang 464000,China

  • Online:2021-03-28 Published:2021-04-01

摘要: 分析荆芥和荆芥穗饮片挥发油中化学成分特征的差异,为其质量评价及临床应用提供依据。本研究收集市场上不同来源的荆芥和荆芥穗饮片,首先,采用水蒸气蒸馏法提取样品总挥发油,并利用GC-MS对荆芥和荆芥穗样品中的挥发油成分及含量进行测定;其次,利用SIMCA14.1软件对荆芥与荆芥穗中挥发油共有成分相对含量进行主成分分析、聚类分析;最后,利用OPLS-DA结合t-test筛选导致荆芥和荆芥穗挥发油存在差异的代谢标志物。结果表明,荆芥中挥发油总含量普遍低于荆芥穗,荆芥和荆芥穗挥发油含量的差异达极显著水平;荆芥中挥发油成分种类多于荆芥穗,荆芥与荆芥穗挥发油成分特征存在差异,各自聚为一类;OPLS-DA结果结合t检验共筛选出薄荷酮、异胡薄荷酮、β-石竹烯、柠檬烯、β-蒎烯、胡薄荷酮、香芹醇、顺-p-2,8-薄荷二烯醇、1-辛烯-3-醇、4-异丙烯基甲苯10种引起两种饮片差异的主要代谢标志物。因此,荆芥与荆芥穗两种饮片挥发油的化学成分特征存在差异,该差异与其临床功效的相关性还有待进一步研究。


关键词: 荆芥, 荆芥穗, 挥发油, GC-MS, PCA

Abstract:

Schizonepetae Herba and Schizonepetae Spica were analyzed to provide a basis for its quality evaluation and clinical application.In this study,Schizonepetae Herba and Schizonepetae Spica were collected from different sources on the market.First,the total volatile oil of the samples was extracted by steam distillation,and the volatile oil composition and content in Schizonepetae Herba and Schizonepetae Spica samples were determined by GC-MS;Second,principal component analysis and cluster analysis were used on the relative content of the common components of the volatile oil in Schizonepetae Herba and Schizonepetae Spica by the SIMCA14.1 software.Finally,OPLS-DA combined with t-test were used to test and screen differential metabolic markers of volatile oil in Schizonepetae Herba and Schizonepetae Spica.The result shows that the total content of volatile oil in Schizonepetae Herba is generally lower than Schizonepetae Spica,and it is extremely significant that the content of volatile oil in Schizonepetae Herba and Schizonepetae Spica is different.Moreover,the kind of volatile oil in Schizonepetae Herba is more than that in Schizonepetae Spica.Schizonepetae Herba and Schizonepetae Spica are differences in composition characteristics,and they are grouped into one category,respectively.The OPLS-DA results combined with t-test indicates that there are 10 kinds of main metabolic markers including menthone,isopulegone,β-caryophyllene,limonene,β-pinene,pulegone,carvol,cis-p-2,8-menthol,1-octene-3-ol and 4-isopropenyl toluene that cause the difference between the two pieces of decoction.Therefore,there are differences in the chemical quality characteristics of the volatile oils of Schizonepetae Herba and Schizonepetae Spica,and the correlation between the difference and its clinical efficacy remains to be further studied.

Key words: Schizonepetae Herba, Schizonepetae Spica, volatile oil, GC-MS, PCA

中图分类号:  R286.0