天然产物研究与开发 ›› 2021, Vol. 33 ›› Issue (3): 394-401.doi: 10.16333/j.1001-6880.2021.3.006

• 研究简报 • 上一篇    下一篇

草甘膦对茶树叶片主要生化成分的影响

郭永春1，王淑燕1，王鹏杰1，陈金发3，周鹏4，欧阳立群4，赵峰2*，叶乃兴1*   

  1. 1福建农林大学园艺学院/茶学福建省高校重点实验室，福州 350002；2福建中医药大学药学院，福州 350122；3国家茶叶质量安全工程技术研究中心，安溪 362400；4福建省产品质量检验研究院，福州 350002

  • 出版日期:2021-03-28 发布日期:2021-04-01
  • 基金资助:
    福建省“2011协同创新中心”中国乌龙茶产业协同创新中心专项（闽教科〔2015〕75号）；国家茶叶质量安全工程技术研究中心开放课题（2018NTQS0301）；福建农林大学优秀硕士学位论文资助基金（2019S01）

Effect of glyphosate on main biochemical components of tea leaves

GUO Yong-chun1,WANG Shu-yan1,WANG Peng-jie1,Chen Jin-fa3,ZHOU Peng4,OUYANG Li-qun4,ZHAO Feng2*,YE Nai-xing1*#br#   

  1. 1College of Horticulture,Fujian Agriculture and Forestry University/Key Laboratory of Tea Science in Universities of Fujian Province,Fuzhou 350002,China;2College of Pharmacy,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China; 3National Engineering Research Center for Tea Quality-and-Safety,Anxi 362400,China; 4Fujian Inspection and Research Institute for Product Quality,Fuzhou 350002,China

  • Online:2021-03-28 Published:2021-04-01

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摘要： 以金观音茶树为试材，将草甘膦定量施于栽培基质，通过观察叶片的表观药害，并采用超高效液相串联质谱方法测定草甘膦施用前（0天）和施用后（7天和33天）叶片中的农药残留（草甘膦及氨甲基膦酸）及生化成分（游离氨基酸、儿茶素和生物碱）的含量变化，从而科学地评估草甘膦的使用对茶叶质量安全的影响。结果表明：草甘膦施用33天，茶树叶片未见明显药害表征，但可检测到草甘膦及其代谢物氨甲基膦酸；草甘膦施用后，游离氨基酸和儿茶素总量降低，生物碱总量先升后降；多元统计分析表明，草甘膦施用前后样品组间的茶氨酸、咖啡碱、表没食子儿茶素没食子酸酯、谷氨酰胺、表儿茶素没食子酸酯、表儿茶素、精氨酸、天冬氨酸、谷氨酸和表没食子儿茶素含量存在显著差异。综上，茶园草甘膦的施用不易使茶树叶片产生明显的药害表征，但可在茶树叶片中残留较长时间，并显著改变茶树叶片中游离氨基酸、儿茶素和生物碱类化合物的含量。因此，建议茶园尽量不使用草甘膦除草剂。


关键词: 茶树, 草甘膦, 生化成分, 氨基酸, 儿茶素, 生物碱

Abstract:

Taking Camellia sinensis‘Jin-guanyin’ as test material,glyphosate was quantitatively applyed on tea plants cultivation substrate.By observing the apparent phytotoxicity of tea leaves,and using ultra-high performance liquid phase tandem mass spectrometry to determine the pesticide residues (glyphosate and aminomethylphosphonic acid) and main quality components (free amino acids,catechins and alkaloids) in the tea leaves before (0 d) and after (7 d and 33 d) glyphosate applying,to explore the impact of glyphosate application on the quality and safety of tea production.The results showed that:glyphosate was applied for 33 days,and there was no obvious sign of phytotoxicity in tea leaves,but glyphosate and its metabolite aminomethylphosphonic acid were still detected;The total amount of free amino acids and catechins decreased after the application of glyphosate,while the total amount of alkaloids first increased and then decreased;Multivariate statistical analysis showes,there are obvious differences of theanine,caffeine,epigallocatechin gallate,glutamine,epicatechin gallate,epicatechin,arginine,aspartic acid,glutamic acid and epigallocatechin between the sample groups before and after glyphosate application.In summary,the application of glyphosate in tea gardens is not easy to cause phytotoxicity in the tea leaves,but it can remain in the leaves of tea plants for a long time and significantly change the content of free amino acids,catechins and alkaloids in tea leaves.

Key words: Camellia sinensis, glyphosate, biochemical components, amino acids, catechins, alkaloids

中图分类号:  S571.1

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