Jianghuang (JH) could be effective for the treatment of various diseases.The compatibility laws of blood-regulating formulae containing JH from the Chinese Traditional Medicine Formula Dictionary were analyzed using a generalized rule induction (GRI) algorithm implemented.System pharmacological mechanisms are traced by leveraging data mining of bioinformatics databases and MTT cell proliferation assay,Elisa and Western blotting analysis.We found that the JH-CX-DG formula (Jianghuang-Chuanxiong-Danggui) could represent a key formula containing JH in blood-regulating TCM formulae.The PPI network and KEGG pathway enrichment analysis showed that the JH-CX-DG formula possess potential pharmacological effects including anti-inflammatory,improving microcirculation,and anti-tumor through the regulation of multiple pathways including PI3K/Akt,MAPK,Toll-like receptor,T cell receptor,EGFR,VEGFR,Apoptosis,HIF-1 (P<0.05).The MTT assay,Elisa and Western blotting showed that the “JH-CX-DG” formula can inhibit human lung cancer A549 cells proliferation and down-regulate the expression of p-EGFR,p-PI3K,p-Akt,VEGF and HIF-1α.The JH-CX-DG formula can exert beneficial pharmacological effects through multi-target and multi-pathway interactions.It maybe be effectively administered for the treatment of inflammatory diseases,microcirculation disorders,cardiovascular disease,and cancer.We found a new effective drug formula through analyzing the compatibility law and systemic pharmacological mechanism of JH.Our study provides a theoretical basis and directions for subsequent research on the JH-CX-DG formula.