Abstract: In order to study the preparation of Perilla frutescens antibacterial agent and its antibacterial mechanism against aspergillus Niger and staphylococcus aureus.The composition of Perilla frutescens antibacterial agent was studied by orthogonal experiment.The inhibitory effect of Perilla frutescens antibacterial agent was studied by Oxford cup method and minimum inhibitory concentration.The antibacterial mechanisms of Perilla frutescens antibacterial agent against aspergillus Niger was studied by measuring the PH,electrical conductivity,ion,RNA and protein leakage of aspergillus Niger and the ergosterol content.By detecting the extracellular conductivity of staphylococcus aureus,the intracellular leakage of reducing sugar,RNA and protein ions,the content of peptidoglycan,and the damage to the cell wall,the antibacterial mechanism of Perilla against staphylococcus aureus was studied.The results showed that the best ratio of Perilla frutescens antibacterial agent was∶fructus perillae∶pogostemon cablin∶sunglo =4∶1∶1;the minimum inhibitory concentration (MIC) for aspergillus Niger and staphylococcus aureus were 0.2% and 0.06%.The main mechanism of action of Perilla frutescens antibacterial agent on aspergillus Niger and staphylococcus aureus was that it destroyed the membrane structure of aspergillus Niger.

Key words: Perilla frutescens antibacterial agent, ratio, Aspergillus Niger, staphylococcus aureus, antibacterial activity, antibacterial mechanism