NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2021, Vol. 33 ›› Issue (7): 1086-1095.doi: 10.16333/j.1001-6880.2021.7.002

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Aronia melanocarpa anthocyanins protect SH-SY5Y cells against Aβ1-42 induced oxidative stress and apoptosis through a Nrf2 mechanism

MENG Ling-shuai1,CAO Sen1,PENG Lin1,MA Li-zhi1,MENG Xian-jun2,ZHOU Xiao-li1*   

  1. 1School of Food and Pharmaceutical Engineering,Guiyang College,Guiyang 550000,China; 2College of Food Science,Shenyang Agricultural University,Shenyang 110866,China

  • Online:2021-07-28 Published:2021-07-29

Abstract:

We investigated the cytoprotective effects of anthocyanins,including cyanidin-3-O-(galactoside,glucoside,arabinoside,xyloside),from Aronia melanocarpa against oxidative stress and apoptosis induced by Aβ1-42.The MTT assay was conducted by MTT colorimetric assay and cell group was done,oxidative stress related indicators,including reactive oxygen species (ROS),hydrogen peroxide (H2O2),superoxide dismutase (SOD),were measured using ROS,H2O22,SOD detection kit,respectively,apoptosis was detected by Annexin-V-PI/FITC apoptosis detection kit.Besides,the gene transcription and protein expression of antioxidase-related gene nuclear factor erythroid (Nrf2),heme oxygenase-1 (HO-1),quinone oxidoreductase (NQO1) and apoptosis-related genes (Bcl-2,Bax) were analyzed by RT-PCR and Western blot.These results showed that anthocyanins pretreatment significantly inhibited Aβ1-42-induced increase in intracellular ROS,H2O2,and increased the SOD expression through upregulating the Nrf2,HO-1,NQO-1 expression.Apoptosis was significantly inhibited by anthocyanins via down-regulation of Bax and up-regulation of Bcl-2.It can be concluded that high-purity Aronia melanocarpa anthocyanins could protect SH-SY5Y cell from Aβ1-42-induced oxidative stress and apoptosis through a Nrf2 mechanism.

Key words: Aronia melanocarpa, anthocyanins, SH-SY5Y cell, oxidative stress, apoptosis

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