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Review on Extraction,Composition and Biological Activities of Polysaccharide from Tricholoma matsutake CHEN Cheng1,LI Qiang2,HUANG Wen-li2* NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2016, 28 (9): 1499-1503.   DOI: 10.16333/j.1001-6880.2016.9.028 Abstract （7059）      PDF（pc） （551KB）（349）       Knowledge map    Save Tricholoma matsutake is a valuable edible and medicinal mushroom with rich nutrition.It contains a large number of biologically active compounds,such as polysaccharides,anthraquinones,phenols,triterpenes and sterol,which were found to possess multi-beneficial medicinal effects,including antioxidant, anticancer, anti-aging,radiation resistance, etc. Polysaccharide is one of the main active constituent with the wide biological activities.Herein,the extraction methods,purification,composition and biological activities of polysaccharide from T.matsutake was reviewed to provide reference for the development of health products and medicine with T.matsutake polysaccharide. Related Articles | Metrics | Comments（0）

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Research Progress on Anti-aging Effect of Total Flavonoids of Herba Epimedii ZHANG Jiali1，DING Hui2，SONG Xinbo1,2* NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2018, 30 (2): 339-343.   DOI: 10.16333/j.1001-6880.2018.2.027 Abstract （5430）      PDF（pc） （579KB）（373）       Knowledge map    Save Delaying aging and prolonging life have been the hot topics worldwide in recent years.Epimedium is considered as a kind of natural anti-aging drug.The main active ingredient is called the total flavonoids,which plays an important role.It can help delay senility because of the antioxidant and anti-inflammatory effects and because it can promote cell proliferation.Therefore,this paper discusses the mechanism of the anti-aging effect of the total flavonoids of epimedium and its related signal transduction pathway,in order to lay foundation to the study of anti-aging drug. Related Articles | Metrics | Comments（0）

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Extraction and in vitro Antioxidant Activity of Anthocyanins Pigment from Vigna angularis (Willd.) Ohwi et Ohashi Hulls LI Fang,RUAN Chang-qing*,TANG Kai-xin,LI Wei-wei,ZHANG Xue-lu,SUN Ji-tao NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2016, 28 (4): 561-567.   DOI: 10.16333/j.1001-6880.2016.4.017 Abstract （5326）      PDF（pc） （711KB）（302）       Knowledge map    Save In order to explore new anthocyanins resources,extraction process for anthocyanin pigments from Vigna angularis (Willd.) Ohwi et Ohashi hulls was developed and optimized by single factor and L9(34) orthogonal experiments.The optimal conditions for the extraction of anthocyanins from V.angularis hulls was 60% ethanol as extraction solvent,solid-liquid ratio of 1:20 (g:mL),temperature of 40 ℃ and pH 2.0.Under these extraction conditions,the highest extraction amount for anthocyanins was 5.912 mg/g with extraction yield of 19.1% and purity of 3.06%.V.angularis hulls crude extract showed a strong O2·-, ·OH and DPPH radical scavenging ability. V. hulls can be utilized as a new type of anthocyanins resource. Related Articles | Metrics | Comments（0）

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Effects of Plumbago zeylanica Analgesic Spray on Cartilage Histopathology of Rabbit Knee Osteoarthritis and the Expression of TNF-α,IL-6,MDA and SOD DU Yi-kuan*,ZENG Guang-long,TAN Zhi-chao,YUAN Sheng-chao,CAI Li-min* NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2017, 29 (12): 2036-2043.   DOI: 10.16333/j.1001-6880.2017.12.007 Abstract （5052）      PDF（pc） （1406KB）（223）       Knowledge map    Save The purpose of this study was to observe the effect of Plumbago zeylanica analgesic spray (PZAS) on the morphological changes of articular cartilage and the expression of inflammatory cytokines and free radicals by using the New Zealand rabbit model of knee osteoarthritis,and to explore the effect of P.zeylanica and its new external method on osteoarthritis,and hence to provide the corresponding pharmacodynamic basis for clinical application.After the successful model of the knee cavity injection of papain,the rabbits were randomly divided into normal control group,model group,voltaren (diclofenac diethylamine) emulgel group, P.zeylanica spray group with high dosage (2 mg/mL),medium dosage (1 mg/mL) and low dosage (0.5 mg/mL).After 6 weeks of drug delivery,the content of serum TNF-α and IL-6 was determined by Elisa,and the articular synovial tissue homogenate was used as MDA (Malondialdehyde,TBA method) and increased SOD (Superoxide dismutase,Hydroxylamine method),the tibial plateau articular cartilage was prepared by paraffin section,and HE staining and toluidine blue staining were observed using Mankin’s standard.The results showed that the analgesic aerosol spray can effectively reduce the inflammatory factor TNF-α and IL-6 value (P<0.05) in the model of knee arthritis in New Zealand rabbits,effectively reduce the value of MDA and increased SOD value (P<0.05) in the synovial membrane;the mean arthritis index was effectively reduced (P<0.05);The high and medium dosage groups were better than the voltaren emulgel group in the cartilage structure,chondrocyte,toluidine blue staining and the integrity of the tidal line (P<0.01 or P<0.05).It is suggested that P.zeylanica analgesic spray can effectively inhibit the development of osteoarthritis,improve the pathological morphology of articular cartilage,and inhibit inflammatory factors,also can resist oxidation,and thus play a therapeutic role. Related Articles | Metrics | Comments（0）

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Chemical Constituents of the Bark of Anthocephalus chinensis XU Xiao-yu, LI Shang-zhen, SONG Qi-shi NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2011, 23 (3): 393-398.   Abstract （5038）      PDF       Knowledge map    Save Ten compounds were isolated and purified from the bark of Anthocephalus chinensis by column chromatography on silica gel, MCI and Sephadex LH-20, and their structures were elucidated as 4-carboxy-3-hydroxy-5-methylphenyl 3-methoxy-4-hydroxy-5-methylbenzoate (1), sitosterol-3-O-(6'-O-palmityl)-β-D-glucoside (2), quinovic acid-3-O-α-L-rhamnopyranoside (3), clethric acid (4), hederagenin (5), uncargenin C (6), morolic acid (7), caffeic acid methyl ester (8), cadambine (9), and 3α-dihydrocadambine (10) by modern spectroscopic methods. Compound 1 was a new phenolic compound, and compounds 2-8 were obtained from Anthocephalus plantsfor the first time. Reference | Related Articles | Metrics | Comments（0）

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Progress in Chemical Constituents and Bioactivities of Psychotria ZHANG Cui-xian,PENG Guang-tian,HE Xi-xin,ZHU Chen-chen,DENG Jie-wei,WEI Yu-long NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2011, 23 (3): 571-576.   Abstract （4919）            Knowledge map    Save Plants from the genus of Psychotria were wildly used as folk medicines in the South China, however the reports on chemical constituents and bioactivities obtained from China were very few. In order to further study the chemical constituents from Genus of Psychotria and enlarge the sources of medicinal plants, a review on chemical constituents and bioactivities on genus Psychotria was finished. Reference | Related Articles | Metrics | Comments（0）

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Preparation of Tanshinone ⅡA Derivatives and Their Proliferation Inhibitory Activity against HeLa Cells JIANG Cheng-feng, LI Rui-feng, XU Dan, YANG Fu-jun, SHEN Xiu, XU Wen-qing NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2012, 23 (3): 385-388.   DOI: A Abstract （4585）            Knowledge map    Save Five derivatives of Tanshinone ⅡA were prepared via Mannich reaction with esterified amino acid. Their structures were characterized by spectral data. The antiproliferative activities of Tanshinone ⅡA and the derivatives against HeLa cells were evaluated by MTT assay. All the five derivatives showed higher activity than Tanshinone ⅡA, which suggested that modification at C-16 of Tanshinone ⅡA could enhance its inhibitory activity on tumor cells. Reference | Related Articles | Metrics | Comments（0）

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Antimicrobial Activity and Mechanism of Flavonoids from Litchi Pericarp ZHOU Wei-Jing, SHI Guo-Han, SUN Zhi-Da, XIE Bi-Jun, LI Shu-Yi NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2011, 23 (2): 332-336.   Abstract （4339）      PDF       Knowledge map    Save The concentration of total flavanoids from litchi pericarp (LPF) was determined by spectrofluorimetry，and its antimicrobial and bactericidal activities against microbes，as well as the mechanisms were investigated. The purity and yield of extract was 48.56% and 13.61%，respectively. Meanwhile，the diameter of inhibition zone of Escherichia coli，Staphylococcus aureus and Saccharomgces cerevislac by oxford plate assay were 15.1，14.0 and 13.8 mm，respectively. The maximum inhibitory rate of Aspergillus niger reached 28.75% under the concentration of 10 mg/mL. The minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of LPF were also measured by plate dilution method. As the results，the MIC of LPF against E. coli and St. aureus were 2.5 mg/mL and MBC were 5 mg/mL，while those of Sa. cerevislac and A. niger were 5 mg/mL and no bactericidal activity observed. Observation under scanning electron microscope revealed that the antimicrobial activity and the bactericidal function of LPF against St. aureus were directly relevant to the structure destruction of cells membrance and wall. Reference | Related Articles | Metrics | Comments（0）

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Chemical Constituents of Capparis spinosa L. ZHAO Jun, YANG Wei-jun, REN Yuan, XU Fang, XU Jian-guo NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2012, 23 (1): 52-54.   DOI: A Abstract （4337）      PDF（pc） （328KB）（519）       Knowledge map    Save Eleven chemical compounds were isolated and purified from Capparis spinosa L.by extraction and different kinds of column chromatography. Their structures were determined on the basis of the physicochemical properties and spectral analysis as octacosane (1), daucosterol (2), 1-octacosanol (3), lacceroic acid (4), hexadecanoic acid (5), butanedioic acid (6), protocatechuric acid (7), 4-hydroxy-5-methylfuran-3-carboxylic acid (8), kaempferol-3-O-?-D-glucopyranoside (9), rutin (10) and stachydrine (11). Compounds 1, 3 and 4 were obtained from Capparis genus for the first time. Related Articles | Metrics | Comments（0）

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Chemical Constituents of Phyllanthus niruri L. ZHU Hong-lin, WEI Wan-xing, ZHOU Min, YANG Dan, FAN Xi-wang, LIU Jian-xiong NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2011, 23 (3): 401-403.   Abstract （4277）            Knowledge map    Save Five compounds were isolated from the aerial part whole plants of Phyllanthus niruri L. and identified as corilagin (1), rutin (2),isobubbialine (3), succinic acid (4),and gallic acid (5) on the basis of physical and chemical properties and spectra data. The assignment of NMR signals of compound 3 was revised, and the NMR signals of compound 1 were assigned with 1D and 2D-NMR spectra. Reference | Related Articles | Metrics | Comments（0）

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Constituents from Heartwood of Streblus asper and Their Antibacterial Activity HUANG Ji-guo, LI Jun, WU Qiang, YANG Rui-Yun, LI Shan, CHEN Zi-zhan, LI Lu-qing NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2012, 23 (6): 780-.   DOI: A Abstract （4123）            Knowledge map    Save Eight known compounds were isolated by various chromatographic techniques from the heartwood of Streblus asper and their structures were elucidated on the basis of spectroscopic analysis, they are ginkgetin (1), 4´-methoxy-7-α-L-rhamnosyl-(1→6)-β-D-glucopyranosy isoflavone (2), rhamnocitrin-3-O-β-D-galactopyranoside (3), β-amyrone (4), β-sitosterol (5), vanillic acid (6), behenic acid (7), and docosenoic acid (8). All compounds were isolated from this plant for the first time except for 5. In the antibacterial activities assay, compound 2 showed high antibacterial activities. Reference | Related Articles | Metrics | Comments（0）

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Chemical Constituents of South China Sea Gorgonian Melitodes squarnata Nutting HUANG Li-si , HE Fei, WANG Ping, ZHANG Xiao-yong, QI Shu-hua NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2012, 23 (4): 432-436.   DOI: A Abstract （4074）            Knowledge map    Save Seventeen compounds were isolated by silica gel column, sephadex LH-20 and HPLC chromatograph, and their structures were identified to be malonganenone E (1), malonganenone D (2), nuttingin A (3), adenosine (4), 1,3,7-trimethyl-xanthine (5), 2′-deoxythymidine (6), 3-methyl-6-hypoxanthine (7), 2′-deoxyuridine(8), (22E,24R)-ergosta-8(14),22-dien-3β,5α,6β,7α-tetrol (9), (22E)-cholest-5,22-dien-3β-ol (10), cholesterol (11), (2S,3S,4R)-N-hexadecanoyl)-2-amino-1,3,4-eicosanetriol (12), (2S,2′R,3R,4E,8E)-N-2′-hydroxytetradecanoyl-2-amino-9-methyl-4,8-octadecaadiene-1,3-diol (13), batyl alcohol (14), cetyl glycerin ether (15), glycerol trioleate (16), and hydroxy-2,3-dimethyl-2-nonen-4-olide (17) on the basis of spectroscopic analysis and comparison with literatures.. Reference | Related Articles | Metrics | Comments（0）

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Advances in Chemical Compositions and Biological Activities of Plumeria rubra HONG Ting, YU Bo, LU Yu, QIU Sheng-xiang NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2011, 23 (3): 565-570.   Abstract （3976）            Knowledge map    Save This review summarized the chemical constituents and biological activities for plant of Plumeria rubra, in order to provide science evidence for further development and utilization of this plant. Reference | Related Articles | Metrics | Comments（0）

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Chemical Studies on Fruiting Bodies of Hercium erinaceus MA Bing-ji, XU Jun-lei, WEN Chun-nan, YU Hai-you, LI Wen, RUAN Yuan, SHEN Jin-wen, MA Jin-chuan NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2012, 23 (09): 1165-.   DOI: A Abstract （3866）            Knowledge map    Save Eleven compounds were isolated from the fruiting bodies of Hercium erinaceus by means of column chromatography. They were identified as D-erythritol (1), D-arabinitol (2), 1-O-β-D-glucopyranosyl -(2S, 4E, 8E, 2′R)-2-N-(2′-hydroxy-pentadecaamide)-9-methyl-4, 8- sphingadienine(3), 3β-hydroxy-ergosta-5, 7, 22-triene (4), 3β-hydroxy-5α, 8α-epidioxyergosta-6, 22-diene (5), stearic acid (6), oleic acid (7), hericenone F (8), hericenone C (9), hericenone E (10) and hericene A (11), respectively. As a result, hericenones are main components in the content and 3 was the first cerebroside isolated from this mushroom. Reference | Related Articles | Metrics | Comments（0）

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Chemical Analysis of Polysaccharides from Taxus cuspidate JIANG Rui-zhi, JIANG Xiang-zhi, CAO chuan-ying, CHEN Ying-hong, WANG Ying, GAO qi-pin NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2012, 23 (4): 440-443.   DOI: A Abstract （3834）            Knowledge map    Save TP was obtained by water extraction and alcohol precipitation from Taxus cuspidate. TP was filtrated through a cellulose ultrafiltration system to give the extra-ultrafiltrate with TP-1 and intro-ultrafiltrate with TP-2. TP-2 was treated with partially acid hydrolysis, then purified by Sephadex G-75 column to obtain the polysaccharides TP-2-1a. TP-2-1a was studied, including the physical and chemical properties, molecular weight, sugar compositions, glycosidic linkage analysis and so on. The molecular weight of TP-2-1a was about 7.0×103 kDa. Suger compositions were Rha, Man, Gal, Glu, GalA and GlcA, in molar ratio of 116.9:1:15.5:1.3:9.9:2.5. The backbone of TP-2-1a were (1→3) and (1→4)-linked galactosyl, (1→4) and (1→6)-linked mannosyl, (1→3) and (1→4)-linked glucosyl, and (1→4)-linked galacturonide, There were side chains attached to O-6 of (1→3)-linked galactoside and O-4 of (1→2)-linked rhamnoside. And the non-reducing terminals were mostly galactose and a few mannose, glucose and rhamnose. This is the first time to obtain the polysaccharides from T. cuspidate. Reference | Related Articles | Metrics | Comments（0）

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Content Determination of Vanillic Acid in Melia toosendan Sieb. DU Yi-hao,LIU jian-hua,LI Ji-long,AN li-qun,YANG Nai-jia NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2011, 23 (6): 1103-1106.   DOI: A Abstract （3689）            Knowledge map    Save In this research,vanillic acid and iso-vanillic acid in Melia toosendan Sieb were identified by TLC.The content of vanillic acid was determined by HPLC.The HPLC system consisted of ODS-C18,methanol-water-acetic acid (25:75:0.5) as mobile phase,flow rate of 1.0mL/min and column tempreture of 30 ℃.The eluate was detected at 260nm.T he TLC spots developed were fairly clear.The control content of vanillic acid was within the range of 1.022～16.352 μg,and there was fine linearship between the sample input and the peak area.The average recovery was 102.45%,r = 0.9998 (n = 6),RSD = 1.22%. Related Articles | Metrics | Comments（0）

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Chemical Constituents from Polygonum capitatum ZHAO Huan-Xin, BAI Hong, LI Wei, WANG Yuan-Shu, LIU Yong-Jun, LIU Ai-Qin NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2011, 23 (2): 262-266.   Abstract （3636）      PDF       Knowledge map    Save Nine compounds were isolated from EtOAc extract of the Polygonum capitatum Buch.-Ham. ex D. Don.. Their structures were identified as quercetin 3-O-(4"-O-acetyl)-α-L-rhamnopyranoside (1)，quercetin (2)，quercitrin (3)，myricetrin (4)，quercetin 3-rhamnoside 2"-gallate (5)，protocatechuic acid (6)，daucosterol (7)，gallic acid (8) and progallin A (9) on the basis of their physical properties and the spectral data. Compound 1 was isolated from the genus Polygonum for the first time，and 4 was isolated from the plant for the first time. The NMR spectroscopic characteristics of the acetylated rhamnose of flavonoids glycosides were summarized. Reference | Related Articles | Metrics | Comments（0）

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Chemical Constituents of the Plant Micromelum falcatum (Lour.) Tan. HUANG Yong-zhong, ZHANG Si, HUANG Sheng, LUO Xiong-ming, WANG Jian-hua NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2012, 23 (09): 1161-.   DOI: A Abstract （3619）            Knowledge map    Save Twelve compounds were isolated from Micromelum falcatum and purified by repeated column chromatography on silica, Sephadex LH-20 gel, and HPLC and structurally identified by spectral analysis. The compounds were identified as 5-formyl-6,7-dimethoxycoumarin (1), isoscoploletin-β-D-glucoside (2), 6-(trans-1-buten-3-only)-7-methoxycoumarin (3), isoscopoletin-(6-hydroxyl-7-methoxyl coumarin, isoscopoletin) (4), microfalcatin isovalerate (5)，micromelin(6), syringin (7), coniferin (8), Methyl 2-O-β-D-glucopyranosylbenzoate (9), 2-hydroxy-5-methoxy-trans-cinnamic acid (10), 3,5-dimethoxyl-4-hydroxyl benzaldehyde (11) and o-sec-butyl phenol (12). Compound 1 previously synthesized but first isolated as natural product, and the other compounds were isolated from this plant for the first time except 5 and 6. Reference | Related Articles | Metrics | Comments（0）

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Advances in Bacteriostatic Activity of Plant Essential Oil HU Lin-Feng, XU Ming-Lu, ZHU Hong-Xia NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2011, 23 (2): 384-391.   Abstract （3587）      PDF       Knowledge map    Save In this paper，advances on bacteriostatic activity and active ingredients of plant essential oil were summarized，which included 31 plant essential oils and 13 active ingredients. In addition，bacteriostatic mechanisms of essential oil and active ingredients were analysed. Reference | Related Articles | Metrics | Comments（0）

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Chemical Constituents from Inula nervosa Wall. YAN Lan, JIN Hui-Zi, NIE Li-Yue, QIN Jiang-Jiang, FU Jian-Jun, ZHANG Wei-Dong NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2011, 23 (2): 258-261.   Abstract （3564）      PDF       Knowledge map    Save Thirteen compounds were isolated from the 75% ethanol extract of the aerial parts of Inula nervosa Wall.. On the basis of spectral data，they were identified as shionone (1)，β-sitosterol (2)，α-spinasterol (3)，ursolic acid (4)，daucosterol (5)，bigelovin (6)，loliolide (7)，24S-ethyl-5α-cholesta-7，22E-dien-3α-ol-β-galactopyranoside (8)，spinacetin (9)，kaempferol (10)，α-spinasterol-3-O-β-D-glucoside (11)，benzyl-β-D-glucopyranoside (12)，and 2-phenylethyl-β-D-glucopyranoside (13). Compounds 1-3 and 6-13 were isolated from this plant for the first time. Reference | Related Articles | Metrics | Comments（0）

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Flavonoids from the Twigs of the Tibetian Medicine Myricaria germanica LA Xiao-qin,ZENG Yang,XU Min,ZHANG Ying-jun NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2011, 23 (4): 596-599.   Abstract （3534）            Knowledge map    Save Eleven flavonoids were isolated from the 60% aq.acetone extract of the air-dried twigs of Myricaria germanica (Tamaricaceae),a famous Tibetian medicine.Their structures were elucidated by spectroscopic analysis and identified as 3,5,4'-trihydroxy-7,3'-dimethoxyflavone (1),3,5,4'-trihydroxy-7-methoxyflavone (2),rhamnetin (3),3,5,7-trihydroxy-4'-methoxyflavone (4),tamarixetin (5),kaempferol (6),quercetin-3-O-β-D-glucoside (7),kaempferol-3-O-β-D-glucuronic acid (8),quercetin-3-O-β-D-glucuronicacid (9),quercetrin (10),and afzelin (11),respectively.All compounds were obtained from M.germanica for the first time,and compounds 4,5,7—11 were obtained from the genus Myricaria for the first time. Related Articles | Metrics | Comments（0）

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Optimization the Substrate of Sesame Cake Fermentation by Aspergillus SHAO Yuan-Long, DONG Ying NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2010, 23 (6): 1088-1092.   Abstract （3485）      PDF       Knowledge map    Save Response surface methodology was used to optimize the substrate composition of improving the antioxidant activity in solid-state fermentation sesame cake by Aspergillus sojae. The effects of maltose, glucose, sucrose, fructose, wheat bran, corn steep, yeast extract, and ammonium sulfate on DPPH radical scavening activity were evaluated by Plackett–Burman design. The results showed that wheat bran, glucose, and ammonium sulfate were the main affecting factors and had significante effects on antioxidant activities. Then, the central composite design and response surface analysis were used to determine the optimal levels of the main factors. The optimized fermentation conditions were as follows: amount of glucose, ammonium sulfate and wheat bran were 1.87%,1.54%, and 2.55%, respectively. Under this condition, the DPPH radical scavening ability was 80.26%，which is higher 37% than that of control. Reference | Related Articles | Metrics | Comments（0）

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Thermal Stability and Decomposition Kinetic of Astaxanthin YUAN Chao, JIN Zheng-Yu NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2010, 23 (6): 1085-1087.   Abstract （3425）      PDF       Knowledge map    Save The thermal stability of astaxanthin was studied by thermogravimetry/differential thermal analysis and the start decomposition temperature of astaxanthin is about 250 ℃. The thermal decomposition kinetics was investigated, which suggested that the activation energy EA is 121.14 kJ/mol and the pre-exponential factor is 3.25×108/min. The existence of oxygen had little effect for the decomposition of astaxanthin, but some high temperature residues were created. Reference | Related Articles | Metrics | Comments（0）

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Purification of Earthworm Protein from Coelomic Fluid and Its Bioactivity ZHANG Yang, WANG Yan-Hong, ZHANG Hua, QIN Xue-Gong, CUI Yu-Dong NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2011, 23 (1): 89-93.   Abstract （3407）      PDF       Knowledge map    Save The coelomic fluid of earthworm Eisenia foetida exhibit extensively biological activity previously. Earthworm was induced by 6-Voltages electric stimulation to secrete coelomic fluid, followed by centrifugation and ultrafiltration to obtain a crude protein mixture. The crude extracts have been purified by means of gel chromatography and anion-exchange chromatography in sequence, as resulted in obtaining a kind of pure protein. Ultraviolet analysis indicated the protein absorption wavelength 280 nm, and SDS-PAGE determined the protein molecular weight 38.6 KDa. The further bioactive study showed that the protein displayed the inhibition effects on the growth of Escherichia coli in 1 h and Staphylococcus aureus in 3 h, as well as lysis effects on chicken red cells (minimum effect concentration 0.39 µg/mL). It suggested that activity of the protein could be come from by a means of damage-cell structure. Reference | Related Articles | Metrics | Comments（0）

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Antioxidant Capability of Epi-catechins and Theaflavins In Vitro by Scavenging Hydroxyl Free Radical TU Yun-fei, YANG Xiu-fang, KONG Jun-hao, ZHANG Shi-kang, ZHU Jue-jin, WANG Yin-feng NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2012, 24 (5): 653-659.   DOI: A Abstract （3397）            Knowledge map    Save In this paper, the scavenge ability of hydrogen free radical for both catechins (groupⅰ) and gallate catechin-theaflavins (groupⅱ) was investigated by the method of quadratic general rotate regression design. The results indicated that the scavenge ability of each group increased along with the increasing content of each component in the system, except for TF-D-G which mainly played important role at the low concentration. In group ⅰ, the scavenge ability of of each component was in order of ECG>EC>EGCG, and the contribution ratio of ECG in this system was more than 43%, which in sharp contrast to negligible component of EGC. As for group ⅱ, the order of each component in the scavenge ability was TF-D-G>TF-M-G>ECG>EGCG>TF, and the contribution ratio of TF-D-G exceeded 50%. Interestingly, the gallate catechins could not effectively play the role of scavenging the hydrogen free radicals when they mixed with theaflavins. And the synergy effect of components in corresponding group was weak. Reference | Related Articles | Metrics | Comments（0）

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Progress in Marine Toxins LI Yong, YANG Yan, WU Ming, LI Li-geng, SHI Qing-wen NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2011, 23 (3): 582-589.   Abstract （3387）            Knowledge map    Save A number of marine toxins with diverse structures and a wide array of fascinating bioactivities have been isolated from marine organism. Progress in the studies of marine toxins was summarized, including puffer fish poisoning, paralytic shellfish poisoning, azaspiracid shellfish poisoning, hepatotoxic shellfish poisoning, amnesic shellfish poisoning, diarrhetic shellfish poisoning, neurotoxic shellfish poisoning, ciguatoxin and miscellaneous marine toxins. Marine toxins play a dominant role in the discovery of useful leads for the development of pharmaceutical agents for the treatment of diverse diseases like cancers, cardiovascular diseases, and central nervous system diseases and so on and also serve as useful tools to exploring biological sciences. Total 67 structures and 70 references were provided. Reference | Related Articles | Metrics | Comments（0）

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Advances in Mechanism of Biological Activities of Plant Essential Oils CHEN Jian-yan, LI Yong-yu, WU Shao-hua NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2012, 23 (09): 1312-.   DOI: A Abstract （3386）            Knowledge map    Save Plant essential oils have multifold biological activities, have been widely used in medical care, food industry, cosmetic, agricultural fields and etc. In this paper, the mechanism of several biological activities of plant essential oils have been summarized, such as antibacterial, anticancer, antineoplastic, antioxidative, delaying senile, preventing and curing cardiovascular diseases, antiviral, anti-inflammatory and many other biological activities. Then, some research emphases and breakthroughs of plant essential oils in the future have been proposed. Reference | Related Articles | Metrics | Comments（0）

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Research Progress on Triterpene Phenolic Acid Esters SUN Meng-Ying, LIANG Jing-Yu, FENG Xu, DONG Yun-Fa, YIN Min, CHEN Yu NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2011, 23 (2): 369-373.   Abstract （3385）      PDF       Knowledge map    Save Triterpenoid acid esters are a class of novel natural products. In this review，the progress in the research of the triterpene phenolic acid esters were reviewed with respect to the plant source，type of structure and biological activiies. Reference | Related Articles | Metrics | Comments（0）

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Effects of Protohermes grandis Extract on Reproductive Capacity and Life-span of Drosophila melangogaste YANG Dong-mei, He Lan, WANG Cheng-feng, LI Jun-nian NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2012, 23 (3): 366-369.   DOI: A Abstract （3309）            Knowledge map    Save The effects of Protohermes grandis extract on the life-span and reproductive capacity of Drosophila melangogasters were examined. The imagoes were collected by ether anesthesia within 8 h after eclosion. Similar sizes of female and male individuals were selected and cultivated in mediums with different concentrations of P. grandis extract. The number of dead individuals was counted once a day until all the D. melanogasters died. The results showed that,the number of first filial generation male D. melanogasters under treatment of P. grandis extract was significantly higher than that of control group（P<0.05）, while number of F1 femal D. melanogasters showed significant difference only at the concentration of 2.500% P. grandis’s extracts (P<0.01). Weight of all D. melanogasters at the emergence of eclosion was highly significantly heavier than that of control D. melanogasters (P<0.01). What’s more, life-span of female D. melanogasters was also highly significantly longer than that of the control group (P<0.01). Our study provided theoretical reference for better use of P. grandis. Reference | Related Articles | Metrics | Comments（0）

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Cytotoxic Constituents from the Root of Ardisia Crispa HUANG Wei, TAN Gui-Shan, XU Kang-Ping, LI Fu-Shuang, LI Zhong-Kui, LIU Yan-Ping NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2010, 23 (6): 949-951.   Abstract （3289）      PDF       Knowledge map    Save Four flavonoids were isolated from the root of Ardisia Crispa. by using chromatographic techniques and their structures were identified as wogonin (1), oroxylin A (2), wogonoside (3), and baicalin (4) by chemical evidence and spectral analysis. All of them were isolated from the genus Ardisia for the first time, and compound 3 exhibited strong cytotoxicity against Bel-7402 cell with IC50 value of 7.64 µg/mL. Reference | Related Articles | Metrics | Comments（0）

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The Maillard Reaction of Chitosan Oligosaccharide with Xylose and the Antioxidant Activity of the Chitosan Oligosaccharide Derivatives SUN Tao, CHEN Chun-Hong, XIE Jing NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2011, 23 (2): 219-223.   Abstract （3289）      PDF       Knowledge map    Save Four kinds of the chitosan oligosaccharide derivatives were prepared though Maillard reaction by heating chitosan oligosaccharide and xylose with different times (4 h and 8 h) and different proportions (the mass ratio of chitosan oligosaccharide and xylose was 1:1 and 1:3)，named CX11-4、CX13-4、CX11-8 and CX13-8，respectively. The pH，absorbance，and fluorescence were determined during the reaction. The chitosan oligosaccharide derivatives were characterized by FTIR and their molecular weight was determined by GPC. Their antioxidant activity was investigated by employing various established in vitro systems，such as hydroxyl/1，1-diphenyl-2-picrylhydrazyl (DPPH) radicals scavenging and reducing power. The results showed all of the derivatives had strong antioxidant activity compared to chitosan oligosaccharide. The antioxidant activity was found to be CX13-4>CX11-4 and CX11-8>CX13-8，which indicated that the antioxidant activity of the chitosan oligosaccharide Maillard derivatives is not only related to the ratio of –NH2 and –OH but also related to the reaction times. Reference | Related Articles | Metrics | Comments（0）

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Physicochemical Properties of a Water-soluble Polysaccharide Fraction SHP-3 Purified from Soybean Hull LAI Fu-Rao, WU Hui, LI Xiao-Feng, REN Yao, WU Lei-Yan, WEN Qi-Biao NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2011, 23 (1): 76-81.   Abstract （3246）      PDF       Knowledge map    Save The water-soluble polysaccharide of soybean hull (SHP) was extracted by hot water and three fractions were obtained by using DEAE-cellulose anion exchange column. The physicochemical properties of the purified fractions SHP-3, eluted with 0.3 M NaOH, were investigated. Gel permeation chromatography showed that the molecule weight of SHP-3 was 45554. GC analysis of the monosaccharide composition showed that the molar ratio of Rah: Fuc: Ara: Xyl: Man: Gal: Glu was 3.55:0.44:11.58:1:7.45:5.12:1.12 and the content of galacturonic acid was 26.71% (wt.%). The ultraviolet spectra indicated that there was no presence of protein in SHP-3. The molar percentages of (1→2) glycosidic linkage, (1→4) glycosidic linkage and (1→6) glycosidic linkage were 11.4%, 68.9% and 19.7%, respectively. Reference | Related Articles | Metrics | Comments（0）

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Antibacterial and Antioxidant Activities of the Leaf Essential Oil of Platycladus orientalis ZHOU Yu-fan, WANG Ji-hua, LIU Zhi-long, WANG Qi, HE Wei, ZHOU Li-gang NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2011, 23 (4): 620-624.   Abstract （3233）            Knowledge map    Save The leaf essential oil of Platycladus orientalis obtained by hydro-distillation was investigated by GC and GC-MS. 8-Propoxy cedrane (15.41%), terpinen-4-ol (12.98%), α-pinene (9.85%), sabenene (9.92%), and 3-carene (6.77%) were the major compounds of the 29 identified components which accounted for 96.64% of the total oil of P. orientalis. The oil was rich in monoterpenoids and sesquiterpenoids. It was assayed to have the broad spectral antibacterial activity with the MBC values ranged from 0.10 mg/mL to 1.0 mg/mL, the MIC values from 0.063 mg/mL to 0.90 mg/mL, and the IC50 values from 0.044 mg/mL to 0.763 mg/mL. The essential oil was subjected to the screening for the antioxidant activity by ferrozine-Fe2+ complex formation and β-carotene bleaching inhibition assays. It inhibited both the formation of ferrozine-Fe2+ complex and β-carotene-linoleic acid oxidation in a dose dependent manner with the IC50 values of 0.409 mg/mL and 0.526 mg/mL, respectively. Related Articles | Metrics | Comments（0）

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Phenolic Constituents from Twigs ofTibetian MedicineMyricariagermanica ZENG Yang, LA Xiao-qin, XU Min, ZHANG Ying-jun NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2012, 23 (09): 1169-.   DOI: A Abstract （3216）            Knowledge map    Save Abstract: Eleven compounds were isolated from the 60% aq. acetone extract of the air-dried twigs of Myricariagermanica(Tamaricaceae), and their structures were identified as ferulic acid (1), coniferol (2), feruloyl glucose (3), isolariciresinol (4), caffeic acid (5), p-hydroxycinnamic acid (6), gallic acid (7), 3,5-dihydroxy-4-methoxybenzoic acid (8), rhododendrol (9), 3-methoxy-4-hydroxybenzoic acid (10)and 3,4,5-trihydroxycinnanic acid (11) on the basis of spectroscopic analysis. All compounds were obtained from this plant for the first time, and1-6 and 8-11 were obtained from genus Myricariafor the first time. Reference | Related Articles | Metrics | Comments（0）

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Chemical Constituents from the Roots of Rhododendron spiciferum WU Zhao-Yuan, LI Rong-Tao NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2011, 23 (2): 253-257.   Abstract （3214）      PDF       Knowledge map    Save Fifteen compounds were isolated from the roots of Rhododendron spiciferum by silica gel and Sephadex LH-20 column chromatography. The structures were elucidated on the basis of spectral characteristics and identified as: (–)-lyoniresinol (1)，(+)-cyclo-olivil (2)，(–)-lyoniresinol-9-O-β-D-glucopyranoside (3)，(–)-lyoniresinol-9-O-β-D-xylopyranoside (4)，eucryphin (5)，3，5，7-trihydroxychromone-3-O-α-L-arabinopyranoside (6)，naringenin (7)，eriodictyol (8)，taxifolin (9)，catechin (10)，trans-taxifolin-3-O-α-L-arabinopyranoside (11)，engelitin (12)，trans-taxifolin-3-O-α-L-arabinofuranside (13)，taraxerol (14)，taraxerol acetate (15). Compounds 1-6，8，9 and 13-15 were obtained from Rhododendron spiciferum for the first time. Reference | Related Articles | Metrics | Comments（0）

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Analysis the Volatile Constituents of Pine Needles from Health and Debility Pinus yunnanensis WU Su-Ran, ZHOU Ping-Yang, LI Zheng-Yue, FU Rui-Jin, YUAN Sheng-Yong, XIAO Chun NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2010, 23 (6): 1048-1052.   Abstract （3213）      PDF       Knowledge map    Save The volatile oils of healthy and debility pine needles of Pinus yunnanensis were collected using head-space adsorption method. Chemical constituents of oils in two states were isolated and identified by using gas chromatography-mass spectrometry GC-MS and computer information searching. The relative contents of the volatile constituents were calculated by ionic flow chromatographic area normalization method. The results showed that the constituents and contents of volatiles changed largely during the process from healthy to debility. There were 15 different constituents and 18 same constituents between two states pine needles. The relative contents of the same constituents of two states pine needles were different. Reference | Related Articles | Metrics | Comments（0）

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Chemical composition from MaotaiDistiller’s Yeast WANG Xing-dong1，MU Ming-yue2，ZHANG Qian-jun1*，ZHOU Qing-di3，WEI Gang4 NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2017, 29 (增刊1): 44-46.   DOI: 10.16333/j.1001-6880.2017.S.010 Abstract （3208）      PDF（pc） （370KB）（180）       Knowledge map    Save MoutaiDistiller yeast were extracted by 75% ethanol.The purification of the extracts were conducted via various methods including normalphase silica gel column chromatography,reversedphase silica gel column chromatography,Sephadex LH20 gel column chromatography,thin layer chromatography and recrystallization.Seven compounds were isolated and identified from MoutaiDistiller yeast,namely,palmitic acid (1),βsitosterol (2),syringic acid(3),4hydroxybenzoic acid (4),ursolic acid (5),lauric acid (6) and Vanillin (7).It is the first time in history to isolate and identify the compounds mentioned above from the MoutaiDistiller yeast. Related Articles | Metrics | Comments（0）

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Synthesis of Sulfonated Baicalein and Its Antioxidative Activity and Interaction with DNA HE Qing, WANG Yan-Chang, HE Yun, ZHANG Zun-Ting, ZHANG Xiao-Qing NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2010, 23 (6): 961-964.   Abstract （3199）      PDF       Knowledge map    Save A sulfonate was introduced to the molecular structure of baicalein by sulfonation reaction and water-soluble flavone sodium baicalein-8-sulfonate was synthesized. Sodium baicalein-8-sulfonate was characterized by IR, 1H NMR, and elemental analyses. And the conditions of sulfonation reaction were optimized. The antioxidative activity of sodium baicalein-8-sulfonate was tested by the method of DPPH (1,1-diphenyl-2-picrylhydrazyl free radical). Furthermore, the interaction of sodium baicalein-8-sulfonate with DNA was studied by fluorescent spectroscopy of EB as a spectral probe in Tris-HCl buffer (pH = 7.4). The result indicated that the optimal sulfonation reaction conditions are the ratio of baicalein and sulphuric acid=1:8 (g/mL), temperature 100 ℃, and time 12 h. The antioxidative activity of sodium baicalein-8-sulfonate was stronger than baicalein, and its interaction with DNA was higher. Reference | Related Articles | Metrics | Comments（0）

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Isolation and Identification of a Water-soluble Polysaccharide from Morinda officinalis YANG Zhen-Min, YI Yong-Tao, GAO Chuan-Chuan, FAN Jian-Qiang, HU Jun, ZHAO Ming-Yue NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2011, 23 (1): 1-5.   Abstract （3180）      PDF       Knowledge map    Save A soluble polysaccharide (MOHP-1) was isolated from the roots of Morinda officinalis How via procedures including extraction, precipitation, deprotein, decolor and separation over anion-exchange. On the basis of FTIR, HPLC, NMR and GC-MS analysis after methylation, MOHP-1 was determined as α-Glcp-(1→2)-[β-Fruf-(2→1)-β-Frucf]n, a polysaccharide with a linear chain of (2→1) linked β-fructofuranosyl residues specifying an inulin-type structure. Reference | Related Articles | Metrics | Comments（0）

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Chemical constituents from Sphallerocarpus gracilis SHI Ming-rui, ZHAO Jian-xi, FENG Zu-fei, LIU Jun-xi, PEI Dong, LIU Ye-wei,QIU Duo-long NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2012, 23 (2): 182-184.   DOI: A Abstract （3179）            Knowledge map    Save Eleven compounds were isolated by silica gel column chromatography from Sphallerocarpus gracilis. Their structures were elucidated on the basis of spectral analysis and identified as falcarinol (1), (3R, 8S)-falcarindiol (2), diplaniol (3), 5-hydroxy-8-methoxy-2H-1-benzopyran-2-one (4), esculetin (5), scopolin (6), xanthalin (7), isoimperatorin (8), daucosterol (9), β-sitosterol (10) and stigmasterol (11). Compounds 1~6 were isolated form Sphallerocarpus gracilis for the first time. Related Articles | Metrics | Comments（0）