Top Read Articles

    Published in last 1 year |  In last 2 years |  In last 3 years |  All
    Please wait a minute...
    For Selected: Toggle Thumbnails
    Chemical composition,antioxidant and anti-inflammatory activities of volatile oil from Turpiniae Folium
    LIU Zhao, LI Li, TANG Yang-qiannan, LIN Li-mei, XIA Bo-hou
    NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2022, 34 (5): 723-738.   DOI: 10.16333/j.1001-6880.2022.5.001
    Abstract1188)      PDF(pc) (1410KB)(13)       Save
    Turpiniae Folium is widely used as a functional food or medicine in China.However,little investigations are performed on the chemical composition and biological activity of its volatile oil.In this work,Turpiniae Folium oil extracts (Supercritical extraction) were systematically characterized for the first time.The low polar substances were identified by GC-MS (methyl esterification and non-methylesterification),and LC-MS method was applied to conduct analysis of the intermediate polar substances.Also,the anti-oxidant (DPPH and ABTS method) and anti-inflammatory (LPS-induced RAW 264.7 cell model) activity of the oil from Turpiniae Folium were performed.80 chemical components were identified by GC-MS (38 for non-methyl esterification and 42 for methyl esterification,respectively),most of which were linoleic acid,γ-sitosterol,squalene,2,2′-methylenebis-(4-methyl -6-tert-butylphenol),trans-9-octadecenoic acid,palmitic acid and other substances.In the positive and negative ion mode of LC-MS,a total of 256 compounds were identified,most of which were terpenes,phenylpropanoids,phenols,flavonoids,alkaloids,coumarins and their derivatives,fatty acyls,semi-terpenoids,fatty acids and other compounds.Moreover,the volatile oil from Turpiniae Folium had good ability to resist DPPH and ABTS free radicals,and could significantly inhibit the release of NO and IL-6 in RAW 264.7 cells induced by LPS in a dose-dependent manner.Generally,these results proved the anti-bacterial,anti-inflammatory,and immunoregulatory effects of the volatile oil from Turpiniae Folium,thus providing a reference for the application of PVO as a food or drug.
    Related Articles | Metrics | Comments0
    Biological characteristics of Pythium ultimum causing damping-off disease of Dendrobium officinale and the screening of antifungal plants against it
    TAN Zhi-yong, ZHOU Zhen-yu, YANG Wen-ming, LI Ya-juan, LU Zhi-hong
    NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2022, 34 (7): 1189-1195.   DOI: 10.16333/j.1001-6880.2022.7.012
    Abstract1023)      PDF(pc) (1567KB)(10)       Save
    The biological characteristics of Pythium ultimum causing damping-off disease of Dendrobium officinale and the screening of antifungal plants against P. ultimum were studied to lay a theoretical foundation for the prevention and control of damping-off disease of D. officinale. The inhibitory effect and biological characteristics of aqueous extracts of different Chinese herbal medicines on the  P. ultimum were determined by mycelium growth rate method.The average mycelial growth rate of the  P. ultimum on the PDA medium was 0.22 cm/h.The optimum temperature was 30 °C,the optimum pH was 6-7,the optimum carbon source were glucose and soluble starch,and the optimum nitrogen source was yeast extract. The light has no significant impact on the growth of mycelium. When the concentration of aqueous extract was 40 mg DW/mL,the inhibition rates of aqueous extract of Eugenia caryophyllata Thunb. and Mentha haplocalyx Briq. were 100% and 96.48%,respectively. The water extracts of E. caryophyllata and M. haplocalyx have good application potential in the control of Dendrobium officinale damping-off disease.
    Related Articles | Metrics | Comments0
    Study on active fraction screening and chemical constituents of Yao Medicine Callicarpa longissima fruits
    ZOU Zhi-qi, YU Ling-ling, PAN Zheng-hong, , NING De-sheng, FU Yu-xia, LI Lian-chun
    NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2022, 34 (11): 1865-1870.   DOI: 10.16333/j.1001-6880.2022.11.007
    Abstract979)      PDF(pc) (607KB)(11)       Save
    To screen the anti-inflammatory and anti-oxidative active fractions from fruits of Callicarpa longissima and study its chemical constituents.The activities of different fractions and compounds from fruits of C. longissima were evaluated by the inhibition of NO production and the multiple antioxidant methods including FRAP,ABTS and DPPH radical scavenging.The compounds were isolated and purified from the active fractions by using various chromatography techniques including silica gel,Sephadex LH-20,MCI,HPLC and recrystallization,and their structures were identified by spectroscopic methods.The results showed that the anti-inflammatory and anti-oxidative activities of the ethyl acetate fraction were stronger than others.Nine compounds,obtained from active fractions,were identified as two flavonoids:chrysosplenetin (1) and pachypodol (2);and seven diterpene phenols:carnosol (3),rosmanol (4),7-methoxyrosmanol (5),7-ethoxyrosmanol (6),carnosic acid-11-methylether (7),rosmadial (8),20-deoxocarnosol (9),respectively.Compounds 3,4,6 were the main compounds,and 1,2,5-9 were isolated from this plant for the first time.Compounds 1-9 have anti-inflammatory effects,among which compounds 34 showed significant anti-inflammatory activity.
    Related Articles | Metrics | Comments0
    Icaritin inhibits survival and migration of basal cell carcinoma cells through Hedgehog signaling pathway
    HU Wen-long, WU Ping-ping, LIU Ling-hua, LIU You-rao
    NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2022, 34 (7): 1091-1097.   DOI: 10.16333/j.1001-6880.2022.7.001
    Abstract920)      PDF(pc) (1692KB)(17)       Save
    The purpose of the present study was to investigate the effect and its mechanism of icaritin on basal cell carcinoma cell line A431. A431 cells were treated with icaritin at different concentrations and/or GANT 61. The viability and migration of A431 cells were assayed by CCK-8 method and Transwell assay. The expression of Hedgehog,Smo,Gli1,Bcl-2,Bax and MMP-9 was detected by qPCR and Western blot. Flow cytometry and Caspase-3 activity assay were used to investigate the effects of icaritin and/or GANT 61 on apoptosis of cells. The results showed that,both icaritin and GANT 61 could significantly inhibit the viability of A431 cells,down-regulate the expression of Hedgehog,Smo,Gli1,Bcl-2,MMP-9 and up-regulate the expression of Bax,enhance the activity of Caspase-3 and mediated the apoptosis,inhibited the migration of A431 cells. However,icaritin could not enhance these effects on the basis of Gant 61. Icaritin could promote apoptosis and inhibit migration by Hedgehog signaling pathway in basal cell carcinoma cells. These results suggest that icaritin may promote the expression of apoptotic-related factors,and mediate the apoptosis,down-regulate the expression of MMP-9,and inhibit the migration of basal cell carcinoma cells mainly by inhibiting Hedgehog signaling pathway.
    Related Articles | Metrics | Comments0
    Extraction technology optimization of dietary fiber from Hippophae rhamnoides
    GUO Jing-jing, LI Ya-xuan, FAN Zi-yi, CHEN Xuan-tong, WANG Qi, FU Qun
    NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2022, 34 (7): 1181-1188.   DOI: 10.16333/j.1001-6880.2022.7.011
    Abstract902)      PDF(pc) (1138KB)(18)       Save
    In order to reuse the processing waste of forest resources,this experiment used Hippophae rhamnoides pomace as raw material,using ultrasonic-assisted enzyme method,and analyzed the effects of four factors,namely cellulase addition,ultrasonic temperature,ultrasonic time and material liquid ratio,on the extraction effect of dietary fiber.With the yield ratio of soluble dietary fiber (SDF) and insoluble dietary fiber (IDF) as the index,response surface methodology was used to analyze the extraction effect of dietary fiber.The results showed that the SDF/IDF was 0.21±0.01,and the swelling capacity and oil holding capacity of total dietary fiber (TDF) were 3.12±0.13 mL/g,(4.47±0.41)% respectively under the optimum conditions of 5% cellulase and ultrasonic treatment at 60℃ for 50 min.Therefore,ultrasonic-assisted enzyme method can effectively improve the extraction efficiency of products,and has certain significance for promoting the development and utilization of Hippophae rhamnoide by-product resources.
    Related Articles | Metrics | Comments0
    Analysis on the difference of chemical constituents in Taxilli Herba from different host plants
    YUAN Jia-huan, WU Nan, WANG Wen-xin, CAI Zhi-chen, YIN Sheng-xin, CHEN Hai-jie, LIU Xun-hong, LI Li
    NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2022, 34 (7): 1119-1128.   DOI: 10.16333/j.1001-6880.2022.7.004
    Abstract875)      PDF(pc) (2804KB)(16)       Save
    In order to study the influence of host plants regarding the synthesis and accumulation of metabolites in Taxilli Herba,Ultra-fast liquid chromatography coupled with triple quadrupole-time of flight tandem mass spectrometry (UFLC-Triple TOF-MS/MS) combined with multivariate statistical analysis was used to analyze the differences of chemical constituents in Taxilli Herba from different host plants.Through the analysis of the multistage tandem mass spectrometry,the characteristic peaks were extracted with mass spectrometry data peak matching,peak alignment,and noise filtering.Principal component analysis (PCA) and partial least-squares discriminant analysis (PLS-DA) were used to analyze the data.The constituents were identified according to MS accurate mass and MS/MS spectrometry fragmentation information,combined with the software of database search,comparison with reference standards and literature.The results showed that the chemicals constituents in Taxilli Herba from 10 host plants were clearly distinguished.A total of 19 chemical constituents were screened and identified.Among them,4 common differential chemical constituents ((+)-Catechin,quercetin 3-O-D-glucuronide,hyperoside,quercitrin) presented different changing laws.Our research may provide basic data for revealing the effect of host plants on the synthesis and accumulation of metabolites of Taxilli Herba and evaluating the quality of Taxilli Herba from different host plants.
    Related Articles | Metrics | Comments0
    Screening of anti-allergic components from Corni Fructus and their effects on RBL-2H3 cells degranulation
    ZHANG Ge-ke, LIU Xue, YANG Cheng, SUN Ya-juan
    NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2022, 34 (7): 1196-1203.   DOI: 10.16333/j.1001-6880.2022.7.013
    Abstract833)      PDF(pc) (1361KB)(15)       Save
    In the present study,the potential anti-allergic constituents in Corni Fructus were investigated. The anti-allergic components of Corni Fructus were preliminarily screened by hyaluronidase inhibition assay. The fractions with high hyaluronidase inhibition activity were further evaluated by the degranulation inhibition test of RBL-2H3 cells in vitro. The anti-allergic components were analyzed by gas chromatography-mass spectrometry (GC-MS). The results showed that the petroleum ether extract of Corni Fructus (COPE) had a significantly anti-hyaluronidase activity with an IC50 value of 3.11 mg/mL. Four fractions with higher anti-hyaluronidase activity were separated by silica gel column chromatography,among which COPE-8 had a more significant inhibitory effect on RBL-2H3 cell degranulation in a dose-dependent manner and showed low cytotoxicity. After treatment with 20 μg/mL COPE-8,the histamine and β-hexosaminidase released from RBL-2H3 cells were reduced by 46% and 39%,respectively. The main active components in COPE-8 were α-amyrin,β-amyrin,and sclareol,by GC-MS analysis. These findings signpost that COPE-8 has remarkable anti-allergic activity and has potential application in the development of anti-allergic active substances.
    Related Articles | Metrics | Comments0
    Fingerprint analysis and Q-maker prediction of salt-fried Phellodendri Chinensis Cortex 
    LIU Xiao-lin, WU Wen-ping, PAN Li-ye, XING Ju-ling, GUAN Yong-he, YANG Xiao-dong, PANG Wei, LI Guo-wei
    NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2022, 34 (10): 1647-1656.   DOI: 10.16333/j.1001-6880.2022.10.002
    Abstract833)      PDF(pc) (2223KB)(20)       Save
    Salt-fried Phellodendri Chinensis Cortex (SPCC) is a traditional Chinese medicine,22 batches of SPCC fingerprints were established,and four common components,3-O-ferulic acid,5-O-ferulic acid,berberine hydrochloride and phellodendrine hydrochloride,were identified.The analysis results of PCA and OPLS-DA showed that the differential component of different batches of SPCC was phellodendrine and berberine.Meanwhile,combined with the results of network pharmacological analysis,it was confirmed that berberine and phellodendrine were also the main active component of SPCC.Berberine and phellodendrine were the active components of SPCC which provided reference for the quality control of SPCC.
    Related Articles | Metrics | Comments0
    Study on the differential changes of primary and secondary metabolites between green and ripe Zizyphus jujuba var. spinosa fruits based on principal component analysis
    YANG Xing-jing, LIU Yan-ru , TANG Zhi-shu , SONG Zhong-xing, CHANG Bai-jin, ZHAO Yan-ting, ZHAO Meng-li
    NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2022, 34 (5): 824-835.   DOI: 10.16333/j.1001-6880.2022.5.011
    Abstract813)      PDF(pc) (3101KB)(11)       Save
    In order to characterize the component differentiation from unripe and ripe Zizyphus jujuba var. spinosa,the approach of mass spectrometric technology was adopted to make the coupled chemometric analysis in this experiment.Firstly,the ultra performance liquid chromatography tandem time-of-flight mass spectrometry (UPLC-Q-TOF-MS/MS) technology was utilized to identify the main components from the unripe and ripe fruits.In addition,32 of the primary and secondary metabolites from the two forms of fruits were characterized,and they were classified into 5 kinds of structures.Next,primary metabolites and secondary metabolites from these two forms of fruits were clustered and their varied components were derived via the principal component analysis (PCA).According to PCA analysis,the samples were divided into two categories and 10 of components were selected as the candidate variables to differentia those two forms of fruits.Then,the candidate components from the primary metabolites to secondary metabolites were enriched in their metabolite pathways and biosynthetic pathways via KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway enrichment analysis.At the same time,the candidate components were determined by a mass spectrometry multiple reaction detection (MRM) based methodology.With a good linear relationship from 0.002 3 to 800.00 μg/mL (r<0.99),the average recoveries were 96.38%-104.15% with the RSDs of 1.16%-2.46% (n=6).According to the established methodology,the effects related components from two growing periods of Zizyphus jujuba var. spinosa were oleanolic acid and jujuboside B,which involved in triterpenoids pathways;the flavor-related components were catechin and epicatechin,which involved in flavonoids pathways.
    Related Articles | Metrics | Comments0
    Study on characteristic chromatogram and content determination of phenols and flavonoids of Viticis Negundo Fructus and Viticis Fructus
    LI Guo-wei, HE Jia-ying, LIU Xiao-han, QIU Yun-jing, ZHONG Chun-lin, WU Wen-ping, SUO Cai-xian, SUN Dong-mei
    NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2023, 35 (3): 379-386.   DOI: 10.16333/j.1001-6880.2023.3.003
    Abstract806)      PDF(pc) (1121KB)(7)       Save
    In order to study the differences between Viticis Negundo Fructus and Viticis Fructus,the characteristic chromatogram and multi-index content determination method was established.Based on the UPLC characteristic chromatogram of Viticis Negundo Fructus, nine chemical components were identified by high resolution mass spectrometry(HRMS) then determined,which were protocatechuic acid A,protocatechualdehyde,4-hydroxybenzoic acid,vanillic acid,isoorientin,orientin,agnuside,vitex and isovitex.The determination of 15 batches of Viticis Negundo Fructus and 12 batches of Viticis Fructus showed that there were differences among these nine components.The means of protocatechuic acid,protocatechualdehyde,4-hydroxybenzoic acid,vanillic acid and agnuside for Viticis Negundo Fructus were higher than Viticis Fructus that isoorientin and orientin were lower,while vitexin and isovitexin were not detected in Viticis Fructus.The UPLC characteristic chromatogram combined with multi-index content determination was applied to compare the differernces between Viticis Negundo Fructus and Viticis Fructus,which provide a reference for their quality control and clinical medication.
    Related Articles | Metrics | Comments0
    Study on the treatment of benign prostatic hyperplasia with modified Guizhi Fuling Pill based on network pharmacology and experiment verification
    LIU Dan, CHEN Ya-fei, LIU Zhe-jun, TANG Tian, YU Shu-jun, LIU Gui-min, TANG Yi-bo, LIU Zhen-quan
    NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2022, 34 (7): 1234-1249.   DOI: 10.16333/j.1001-6880.2022.7.017
    Abstract791)      PDF(pc) (5407KB)(25)       Save
    To explore the potential mechanism of modified Guizhi Fuling Pill (MGFP) in the treatment of benign prostatic hyperplasia (BPH) by network pharmacology and molecular docking.Then,the BPH model rats were established to verify the key targets from molecular docking.First,the candidate components and target information of plant herbs in MGFP were determined through TCMSP and TCMID databases,while the animal drug Hirudo was determined through relevant literature.Then,the drug-candidate component-target network diagram was constructed using Cytoscape 3.7.1.The potential targets of BPH were determined through GeneCards,OMIM,TTD,DisGeNET and DRUGBANK databases.The intersection targets of components and disease were selected and imported into STRING 11.0 platform,and PPI network diagram was constructed with Bisogenet 3.0.0 plug-in.Subsequently,Metascape platform and KOBAS 3.0 database were used to perform GO functional annotation,KEGG pathway enrichment analysis and network diagram drawing.Then,the core components and key targets with higher degree values in the network diagram were screened,which will be used for molecular docking in AutoDock 4.2.6 and PyMOL 2.2.0 software.Finally,BPH rat model was established by castration combined with subcutaneous injection of testosterone propionate and intragastrical treatment.Then,according to the immunohistochemical results,the expression of BAX in the prostate tissue of rats in each group was observed.The results of network pharmacology analysis showed that a total of 133 candidate components of MGFP were screened out,of which quercetin,β-sitosterol,kaempferol,baicalein,and ellagic acid could act on BPH.The main targets were AKT1,BAX,BCL2,JUN,TGFB1,TNF,IL6,VEGFA and EGFR,which are mainly concentrated in PI3K-AKT signaling pathway and VEGF signaling pathway to participate in the processes of apoptosis,angiogenesis,and inflammation.Molecular docking results showed that all core components and key targets could spontaneously bind,and BAX and β-sitosterol had the strongest binding capacity.Animal experiments showed that compared with the model group,the expression of BAX was significantly increased when the MGFP doses were 6.57,13.14 and 26.28 g/kg (P < 0.01).All these indicated that MGFP might mainly act on the target of BAX to treat BPH.
    Related Articles | Metrics | Comments0
    Chemical constituents of Murraya paniculata (L.) Jack.
    WANG Ru-ru, ZHANG Shan-shan, XU Hong-tao, WANG Yong-li, CHOU Gui-xin
    NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2023, 35 (5): 787-797.   DOI: 10.16333/j.1001-6880.2023.5.007
    Abstract750)      PDF(pc) (988KB)(18)       Save
    To study the chemical constituents of Murraya paniculata (L.) Jack, two new compounds, named as murrayanin (1) and 8-demethylnobiletin (2), along with twenty-four known compounds (3-26) were isolated from the 70% EtOH extract of M. paniculata by various chromatographic techniques such as silica gel, ODS, Sephadex-LH 20, Pre-HPLC, their structures was elucidated by spectral data analysis. The known compounds were identified as ficusal (3),lariciresinol-4′-monomethy ether (4),(±)-5′-methoxy-4′-O-methyllariciresinol (5),diospyrosin (6),(-)-9′-O-E-feruloyl-lyoniresinol (7),7-O-methylphellodenol-B (8),osthenon (9),3,4′-dihydroxy-3′,5′-dimethoxyphenylacetone (10),4′-hydroxy-5,7-dimethoxyflavanone (11),cystosiphonin (12),4′-hydroxy-5,7,3′-trimethoxyflavanone (13),5,7,3′,4′,5′-pentamethoxyflavanone (14),2′,4-dihydroxy-3′,4′,6′-trimethoxychalcone (15),2′,3-dihydroxy-4,4′,6′-trimethoxychalcone (16),evofolin B (17),2′-hydroxy-3,4,5,4′,6′-pentamoxychalcone (18),2′-hydroxy-3,4,4′,6′-tetramethoxychalone (19),5,8-dihydroxy-6,7,3′,4′-tetramethoxyflavone (20),3′-hydroxy-5,6,7,8,4′,5′-hexamethoxyflavone (21),5,3′,5′-trihydroxy-7,4′-dimethoxyflavone (22),5,7,3′-trihydroxy-8,4′-dimethoxyflavone(23),8-hydroxy-5,6,7,3′,4′-pentamoxyflavone (23),3′-hydroxy-5,6,7,4′-tetramethoxyflavone (24),5,7,3′,4′,5′-pentamethoxyflavone (25) and 5-hydroxy-6,7,8,3′,4′-pentamethoxyflavone (26),respectively.Compounds 3-8,10-13,15,16,20-24 were obtained from the genus Murraya for the first time,and compound 17 were isolated from M. paniculata for the first time.
    Related Articles | Metrics | Comments0
    Study on the mechanism of Huangqisan in treating Alzheimer′s disease based on network pharmacology and experimental verification
    ZHANG Yun-hui , YANG Meng-lin, ZHOU Xiao-qing, WU Da-hua, LIU Xia, LI Xiang
    NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2022, 34 (12): 2119-2129.   DOI: 10.16333/j.1001-6880.2022.12.016
    Abstract728)      PDF(pc) (2201KB)(10)       Save
    To study the mechanism of Huangqisan in treating Alzheimer′s disease(AD) by network pharmacology.To search the active ingredients and corresponding targets of Huangqisan through the TCMSP databases,Then obtained the targets of AD through the DisGeNET and GeneCards database.Constructed protein interaction network map by String online database.At the same time GO and KEGG enrichment analysis of key targets were carried out by using R language.Induction of PC12 cell injury by adopted Aβ25-35 as AD cell model.Detection of cell viability by MTT assay.Observation of cell morphology and synaptic growth by microscope.Observation of Autophagosomes in PC12 cells by transmission electron microscopy.Detection of reactive oxygen species (ROS) content by using Kits.Finally,ELISA and Western blot were used to verify the main biological processes and signaling pathways predicted by network pharmacology.A total of 44 active ingredients of Huangqisan were screened,134 corresponding targets,102 targets related to Alzheimer′s disease.The top 20 KEGG related signaling pathways and GO analysis of the top 20 biological processes.Cell experiments proved that Huangqisan effectively increased the survival rate and synaptic length of PC12 cells,effectively promoted Aβ25-35 induced PC12 cells autophagosome wrapped damaged mitochondria,reduced the content of inflammatory factors IL-1β,IL-18,TNF-α,reduced ROS level,increased LC3 II/I ratio,up-regulated PINK1,parkin,BDNF protein expression,down-regulated p62,NLRP3 protein expression.Huangqisan had a therapeutic effect on AD by activated PINK1/parkin pathway to promoted mitophagy and reduced ROS levels,thereby inhibited the activation of NLRP3 inflammasome and improved synaptic plasticity.
    Related Articles | Metrics | Comments0
    Study on the metabolites and pharmacokinetics of a chalcone extract from Millettia velutina Dunn
    NI Heng-fan, LIU Ling, WAN Li
    NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2022, 34 (7): 1213-1222.   DOI: 10.16333/j.1001-6880.2022.7.015
    Abstract722)      PDF(pc) (1719KB)(12)       Save
    The UPLC-QE-Orbitrap-MS assay was used to compare the 2,5-dimethoxyfurano[4″,5″:3,4]chalcone (1) samples cultured in rat,mouse,rhesus monkey,Beagle dog and human liver microsomes for 0 min and 60 min in vitro,and compare the plasma,feces,and urine samples of intravenous injection of the compound and blank C57 mice to study its metabolites in vitro and in vivo.It was preliminarily concluded that there were two kinds of metabolites M1 and M2 in vitro after incubation,and five kinds of metabolites M1,M2,M3,M4 and M5 in vivo.M5 was further metabolized from M1,and the metabolite M1 was obtained by organic synthesis. Ultrafast liquid chromatography-mass spectrometry (UFLC-MS/MS) detection method was used to determine the plasma concentrations of 1 and M1.The change trend of the compound and M1 in rats was quantitatively studied and the pharmacokinetic parameters were calculated.Rats were randomly divided into groups and given drugs,and blood was collected at a set series of time points.The plasma concentrations of 1 and M1 were determined.The pharmacokinetic parameters were calculated by DAS2.0 software.The Cmax of 1 was 405.96 μg/L,Tmax=0.083 h,AUC0-t=190.64 μg/(L·h),T1/2=3.74 h.The Cmax of M1 was 281.291 μg/L,Tmax=0.083 h,AUC0-t=561.30 μg/(L·h),T1/2 =3.01 h.These results showed that 2,5-dimethoxyfuran[4″,5″:3,4]chalcone undergoes reduction,demethylation,ring opening,and hydroxylation reactions during the metabolism of the body.The main metabolite is M1,and M1 is further metabolized to M5.This study laid a material foundation for revealing its pharmacological effects.
    Related Articles | Metrics | Comments0
    Chemical constituents from the aerial parts of Ficus tikoua Bur. and their antioxidant activity
    HE Zhi-yun, SHI Song-yun, WEI Xue-jiao, LIU Cheng-xiong, CHEN Jian-feng, ZOU Kun, CHENG Fan
    NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2022, 34 (5): 810-817.   DOI: 10.16333/j.1001-6880.2022.5.009
    Abstract721)      PDF(pc) (755KB)(29)       Save
    To study the chemical constituents and antioxidant activity of the aerial parts of Ficus tikoua,a folk medicine of Tujia nationality.Its ethanol extract was separated and purified using silica gel column chromatography,gel column chromatography,macroporous resin column chromatography combined with semi-preparative high performance liquid chromatography and other methods.The structure of the compounds was identified by nuclear magnetic resonance spectroscopy,mass spectrometry and other methods,and seventeen compounds were separated.These compounds were identified as alpinumisoflavone (1),(+)-catechin (2),nodakenin (3),psoralen (4),bergapten (5),(+)-lyoniresinol-3α-O-β-D-glucopyranoside (6),(+)-lyoniresinol-3α-O-β-D-xylopyranoside (7),(-)-isolariciresinol-9-O-β-D-glucopyranoside (8),ssioriside (9),huazhongilexin (10),(+)-isolariciresinol (11),(7R,8S)-3,5′-dimethoxy-4′,7-epoxy-8,3′-neolignane-5,9,9′-triol (12),6,7-dimethoxy-4-hydroxy-1-naphthoic acid (13),ethy-3,4-dihydroxybenzoate (14),bluemenol A (15),3,3′,4,4′-tetrahydroxy diphenyl (16),ethyl chlorogenate (17).Compounds 1-4,6-17 were isolated from this plant for the first time.Three indicators of DPPH radical scavenging rate,total antioxidant capacity,and superoxide anion scavenging capacity were used to determine the antioxidant activity of 17 compounds.The results show that lignans 9,10,and polyphenols 13,14,16 have significant DPPH free radical scavenging ability and total antioxidant ability,and their antioxidant activity is equivalent to that of ascorbic acid at the same concentration.
    Related Articles | Metrics | Comments0
    Progress in biosynthesis and application of L-ergothioneine
    MUKAIDAISI·Maihemuti, CHEN Jian, JIAO Chun-wei, XIE Yi-zhen
    NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2022, 34 (4): 713-721.   DOI: 10.16333/j.1001-6880.2022.4.019
    Abstract706)      PDF(pc) (865KB)(16)       Save
    L-Ergothioneine (L-EGT) is a sort of uncommon thimidazole amino acid with strong antioxidant activity,which cannot be degrated at high temperature and pH.L-EGT only can synthesized by some bacteria,cynobacteria and fungi,but plants,animal and human unable to synthesized.Mammals absorb L-EGT via transporter OCTN1 which distribution in different tissues,thus diverse tissues may influence concentration of L-EGT.With the unique physiological and pharmacological activities made it have extensive application prospect in the field of medicine,food,health care products and cosmetics.Here,we reviewed the recent advances in biosynthetic pathway of L-EGT,and in-depth exploration its roles in food,human health and diseases.
    Related Articles | Metrics | Comments0
    Chemical composition analysis of scammony resin by UHPLC-Q-Orbitrap-HRMS
    KAHAR Gulmira, ABDULLA Rahima, LUO Yu-qin, WU Tao, AISA Hajiakber
    NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2023, 35 (3): 427-443.   DOI: 10.16333/j.1001-6880.2023.3.009
    Abstract690)      PDF(pc) (1377KB)(15)       Save
    The rapid analysis method for the chemical compositions in scammony methanol extract was established by UHPLC-Q-Orbitrap HRMS.The mass data were acquired by Full MS/dd-MS scan mode,and data analysis were performed by the information of accuracy molecular weight,fragment ion,summarized fragmentation pathway,combined with previous references and Compound Discovery(CD) software.Totally,92 compounds were identified from scammony methanol extract,including 72 resin glycosides,12 hydroxyl cinnamoyl quinic acids,eight ether insoluble resin glycoside acids.Among them,eight ether insoluble resin glycoside acids (compounds 10,12,13,16,17,18,19 and 20) and two tricaffeoylquinic acids (compounds 14 and 15) were detected first in scammony.The results provide a scientific basis for the future research on the improvement of the quality standard for scammony and its relative products.
    Related Articles | Metrics | Comments0
    Analysis on the difference of components in different parts of peony root
    ZHANG Shu-rong, ZHAO Hong-su, OU Ying-xue, ZHAI Hong-yan, WU De-ling, ZHANG Cun, ZHANG Wei
    NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2023, 35 (2): 250-259.   DOI: 10.16333/j.1001-6880.2023.2.009
    Abstract671)      PDF(pc) (1942KB)(7)       Save
    This study compared the compositional differences of five peony roots in the processing process:moutan bark,moutan bark removed cork,cork,root core and fibrous root,explores the scientificity of the medicinal parts of peony,and evaluates the value of the development and utilization of the non medicinal parts of the root of peony.The characteristic chromatograms of different parts of peony root were established by ultra-high performance liquid chromatography.The chemical components in different parts of peony root were analyzed by pattern recognition techniques such as hierarchical clustering analysis (HCA),principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) in chemometrics.Seven of the ten differential components selected can be identified and the chemical components with content greater than 0.1 mg/g are used as quantitative analysis indicators for component content determination.The chemical constituents of moutan bark,moutan bark removed cork and fibrous root were similar by characteristic chromatogram and chemometrics analysis;The chemical constituents of the root core and cork were significantly different from the first three.The chemical constituents in the root core were less and the content was lower;The chemical constituents of cork are the most abundant,and monoterpenes and glycosides account for a large proportion.This study shows that there is no significant difference in the principal components between moutan bark,moutan bark removed cork and cork,which provided a basis for the processing of Moutan Cortex in the producing area to leave cork and remove root core;The fibrous root and root core of peony also have certain medicinal value,which can be considered for comprehensive development and utilization.
    Related Articles | Metrics | Comments0
    Phytotoxic eremophilane sesquiterpenes isolated from Ligularia cymbulifera
    CHEN Jia, LI Ming-ming, XIN Hai-jing, ZHENG Guo-wei, PU Shi-biao
    NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2022, 34 (4): 570-578.   DOI: 10.16333/j.1001-6880.2022.4.004
    Abstract667)      PDF(pc) (1040KB)(23)       Save
    Sesquiterpenes were the main allelopathic active ingredients in Ligularia plants and often exhibit phytotoxic activity.In order to find the major compounds from allelochemicals for developing the eco-friendly natural herbicides,the phytotoxic eremophilane sesquiterpenes in Ligularia cymbulifera were studied.Thirteen eremophilane sesquiterpenes were isolated and purified by several chromatographic techniques from the root of L. cymbulifera.Their structures were identified by physicochemical property and analysis of spectroscopic data,including a new eremophilane sesquiterpenes,ligulacymlactam A(1),3β-angeloyloxy-8β-hydroxyeremophil-7(11)-ene-12,8α(14β,6α)-diolide(2),8β-hydroxyeremophil-7(11)-en-12,8α-olide(3),10β-hydroxy-8β-methoxyeremophilenolide(4),6β,8β-dihydroxyeremophil-7(11)-en-12,8α-olide(5),8β-H-eremophil-3,7(11)-dien-12,8α;15,6α-diolide(6),8β-hydroxyeremophil-3,7(11)-diene-8α,12(6α,15)-diolide(7),8β-methoxyeremophil-3,7(11)-diene-8α,12(6α,15)-diolide(8),eremophil-7(11)-ene-12,8α,14β,6α-diolide(9),8β-hydroeremophil-7(11)-en-12β,8α(14β,6α)-diolide(10),3-(methacryloyloxy)furanoeremophilan-15,6α-olide(11),furanoeremophilan-14,6α-olide(12),10β-hydroxy-6β-isobutyrylfuranoeremophilane(13.The phytotoxic activities against A. thaliana of compounds 1-13 were measured by agar germination method.None of them showed inhibitory activities on seed germination,but some of them could promote the germinate time earlier than blank about 1-2 days.As well as,all compounds showed weak root growth inhibitory activity,and compound 13 was the most active compound (EC50 =44.15±5.21 μg/mL).Further structure-activity relationship analysis suggests that the furanermophilane sesquiterpenes which containing the 10-position hydroxyl group and the 6-position side chain substitution have potential to be the lead compound for eco-friendly natural herbicides.
    Related Articles | Metrics | Comments0
    Mechanism of apoptosis induced by exopolysaccharides from Fusarium graminearum on SGC-7901 cells#br#
    #br#
    WANG Jiao-jiao, YUAN Ping-chuan, XU Han-chi, CHEN Ying, ZHANG Si-jia, TIAN Meng-yun, YU Zi-qi, LIU Chun-yan
    NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2022, 34 (7): 1204-1212.   DOI: 10.16333/j.1001-6880.2022.7.014
    Abstract661)      PDF(pc) (1886KB)(7)       Save
    To investigate the effect and mechanism of exopolysaccharides from Fusarium graminearum (FGEPS) on human gastric cancer SGC-7901 cells.Fusarium graminearum was fermented in liquid medium.The exopolysaccharides were separated by alcohol precipitation,decolorization,deproteinization and dialysis,and analyzed by high performance gel permeation chromatography (HPGPC),infrared (IR) and ultraviolet (UV) spectroscopy.CCK-8 method was used to detect the proliferation inhibition activity of SGC-7901,BGC-823,and MGC-803 cells.Effects of FGEPS on apoptosis of SGC-7901 cells were analyzed by Annexin V-FITC/PI,JC-1 and Hoechst 33258 staining.The expressions of pro-apoptotic proteins Bax,Cleaved Caspase-3,Cleaved Caspase-9 and anti-apoptotic protein Bcl-2 were detected by Western blot.FGEPS was isolated from the fermentation broth of Fusarium graminearum,which had the characteristic absorption of polysaccharides and contained four components. In vitro anti-tumor activity assay showed that FGEPS inhibited SGC-7901 cells more significantly than BGC-823 and MGC-803 cells.FGEPS could significantly reduce the mitochondrial membrane potential of SGC-7901 cells,induce the concentration of nuclear chromatin,and produce apoptotic bodies (P<0.05 or P<0.01).FGEPS could raise the expression of pro-apoptotic proteins Bax,Cleaved Caspase-3 and Cleaved Caspase-9 in SGC-7901 cells (P<0.01 or P<0.001),and reduce the expression of anti-apoptotic protein Bcl-2 (P<0.001).In summary,FGEPS isolated from the fermentation broth of Fusarium graminearum could induce apoptosis of SGC-7901 cells through mitochondrial pathway.
    Related Articles | Metrics | Comments0
    Study on chemical constituents and antibacterial activity of Carpesium faberi
    CHEN Jie, WANG Dan, ZHANG Xiong, PENG Ming-you, XU Ran, YAN Ying, WANG Ying
    NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2022, 34 (4): 598-605.   DOI: 10.16333/j.1001-6880.2022.4.007
    Abstract658)      PDF(pc) (687KB)(25)       Save
    In order to study the chemical composition and antibacterial activity of Carpesium faberi, 14 compounds were obtained from the ethanol extract of C. faberi by applying various chromatographic techniques.Their structures were elucidated and identified as granilin (1),4α,10α-dihydroxy-1βH,5βH-guai-11(13)-en-12,8α-olide (2),4β,10β-dihydroxy-5α(H)-1,11(13)-guaidien-8α,12-olide (3),4-epi-isoinuviscolide (4),11(13)-dehydroivaxillin (5),carabrone (6),vomifoliol (7),cassipourol (8),4′,5′-dehydrodiodictyonema A (9),(3R)-8-Hydroxylinalool (10),cholest-7-en-3β-ol (11),stigmasterol (12),5,8-epidioxy-5α,8α-ergosta-6,22E-dien-3β-ol (13),stigmasta-5,22-diene-3β,7α-diol (14).Among them,compounds 2 and 7-14 were obtained from this plant for the first time.The antibacterial activity of compounds 1-14 was preliminarily evaluated,and compound 1 showed weak activity against Bacillus subtilis;compounds 6 and 10 showed weak activity against Candida albicans.
    Related Articles | Metrics | Comments0
    Analysis of chemical components in alcohol extract of Rosa sertata×Rosa rugosa based on UPLC-Q-TOF-MS
    DU Shao-bo, CHEN Xiao-wen, DONG Zhi-qiang, XIE Hui-chun
    NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2022, 34 (7): 1129-1142.   DOI: 10.16333/j.1001-6880.2022.7.005
    Abstract644)      PDF(pc) (1604KB)(24)       Save
    In order to quickly analyze and identify the chemical components of the alcohol extract of Rosa sertata×Rosa rugosa,ultra performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) was used in this study,Waters ACQUITY UPLC HSS T3 column (100 mm×2.1 mm,1.8 μm) was used with The mobile phase is 0.1% formic acid aqueous solution (A) and 0.01% formic acid acetonitrile solution (B) and gradient elution.The MSE data were collected under the negative ion condition of electrospray ion source (ESI).Sixty-three main components were detected according to the exact relative molecular mass,mass spectrometry fragmentation information,and related literature and reference materials,including 26 flavonoids,11 polyphenols(4 phenolic acids and 7 tannins),7 triterpenoids,4 phenylpropanoids,8 organic acids,1 iridoid terpene,1 volatile oil and 5 unidentified components.Twenty-eight compounds such as pectolinarin,melilotoside and asiatic acid were identified in this genus for the first time.UPLC-Q-TOF-MS method was used to further improve the chemical constituents of Rosa sertata×Rosa rugosa,which provided a reference basis for the basic study of pharmacodynamic substances.
    Related Articles | Metrics | Comments0
    Research progress on hypoglycemic effect and mechanism of saponins
    WANG Yue, SU Rong, LIU Zhen-hua, DONG Qi
    NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2023, 35 (1): 159-170.   DOI: 10.16333/j.1001-6880.2023.1.017
    Abstract613)      PDF(pc) (2136KB)(4)       Save
    Diabetes is a metabolic and endocrine disorder,which poses a threat to human health with the increasing incidence of diabetes.In recent years,saponins have attracted wide attention because of their hypoglycemic activity.The regulation of hypoglycemic activity of saponins can be achieved through multiple targets and signal pathways,including insulin signaling pathway,carbohydrate-based metabolic pathway,endoplasmic reticulum stress regulatory pathway,PPAR regulatory pathway and free fatty acid promoting insulin secretion and multiple pathways.Starting with the hypoglycemic signal pathway,this article reviews the hypoglycemic effect and mechanism of saponins in recent ten years,in order to provide reference for the further development and application of hypoglycemic activity of saponins,as well as the treatment and prevention of diabetes.
    Related Articles | Metrics | Comments0
    Extraction optimization,structure characterization and anti-inflammatory activity of polysaccharides from Sparassis subalpina
    LUO Dian-hui, ZHANG Ze-tong
    NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2022, 34 (7): 1110-1118.   DOI: 10.16333/j.1001-6880.2022.7.003
    Abstract606)      PDF(pc) (2260KB)(26)       Save
    To study the isolation,purification,structure properties and anti-inflammatory activities of polysaccharides from Sparassis subalpina.Response surface methodology was used to obtain the optimal processing conditions of total sugar from S. subalpina and the method of column chromatography was used for the purification of a S. subalpina polysaccharide (SSP).Using gas chromatography,NMR spectroscopy and electron microscopies,the structure was studied.Anti-inflammatory activity was determined using RT-qPCR,et al.The results showed that the experimental yield was (18.21 ± 0.68)% under the optimal processing conditions (liquid to solid ratio 80 mL/g,extraction time 60 min,extraction temperature 70 ℃).The average molecular weight of SSP was calculated to be 50 kDa and SSP consisted of glucose,mannose,galactose and arabinose with a molar ratio of 6.5∶1.3∶1∶1.The repeated unit in SSP was determined as →3)-α-D-Galp-(1→2)-β-D-Glup-(1→3)-β-L-Araf-(1→3)-α-D-Manp-(1→,and SSP showed a non-branched and netted state.SSP demonstrated significant inhibitory effects on the gene expressions of TNF-α,COX-2 and iNOS in LPS-stimulated RAW 264.7 cells (P<0.05).The results evidenced the potential effects of SSP with the non-branched and netted state on anti-inflammatory activities.
    Related Articles | Metrics | Comments0
    Toddalia asiatica promotes lipopolysaccharide-induced autophagy and inhibits inflammation in RAW 264.7 cells based on the PI3K/AKT/mTOR pathway
    ZHANG Zong-xing, JIANG Lu, LIU Dao-zhong, HOU Zi-ming, TIAN Meng-jie, TAO Bo-nan, FENG Jia, YUAN Lin
    NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2023, 35 (4): 573-583.   DOI: 10.16333/j.1001-6880.2023.4.003
    Abstract598)      PDF(pc) (1863KB)(5)       Save
    The root of the Rutaxaceae plant Toddalia asiatica (L.) Lam is a natural Tujia Chinese herbal medicine with anti-inflammatory,anti-rheumatic,anti-tumor,anti-microbial and other pharmacological activities.Its characteristics of small poisonous side effects and remarkable curative effect make it a hot spot in current research.Many natural products had been shown to inhibit inflammation and autoimmune diseases by targeting PI3K/AKT/mTOR mediated autophagy.In this study,the effect of TAAE on autophagy was studied by regulating PI3K/AKT/mTOR signaling pathway,and RAW 264.7 cells were induced by LPS to establish an inflammatory model.Cytotoxicity detection kit was used to detect the effect of TAAE on cell viability,the concentration and intervention time of the drug were screened out.Transmission electron microscopy and MDC staining were used to detect the biological function of macrophages.The levels of inflammatory factors in the supernatant were detected by ELISA,and the expression levels of autophagy and pathway-related proteins were detected by Western blot.The effects of autophagy on inflammation and signaling pathways were further verified by using an early autophagy inhibitor (3-MA) and pathway PI3K agonist (740Y-P).The results indicated that TAAE may inhibit the PI3K/AKT/mTOR signaling pathway,promote the formation of autophagic vesicles,fusion and degradation of autophagosomal lysosomes,and reduce the expression and secretion of inflammatory cytokines in RAW 264.7 cells treated with LPS.Overall,the results of this study provide a new clue for the research of the anti-inflammatory mechanism of Toddalia asiatica,and provide a theoretical basis for better clinical application of Toddalia asiatica in the treatment of inflammatory diseases.
    Related Articles | Metrics | Comments0
    Study on the mechanism of Coptidis Rhizoma and Pinelliae Rhizoma in treating chronic atrophic gastritis
    XIANG Yang, HUANG Qiong, YUAN Lin,
    NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2022, 34 (11): 1822-1831.   DOI: 10.16333/j.1001-6880.2022.11.002
    Abstract569)      PDF(pc) (2356KB)(7)       Save
    This study is based on molecular docking and animal experiments to investigate the mechanism of Coptidis Rhizoma and Pinelliae Rhizoma in the treatment of chronic atrophic gastritis.First,the main components of Coptidis Rhizoma and Pinelliae Rhizoma were screened in the TCMSP database,and molecular docking was carried out with IL-1β,IL-6,YAP1,STAT3,JAK2,VEGF,and EGFR using vina software; Secondly,a compound method was used to establish a rat model,and the rats were randomly divided into model (B) group,vitrein (C) group,and low-,medium- and high-dose (D,E,F) groups,with 10 rats in each group.After successful modeling,groups D,E,and F were given HL and BX decoction (1∶2∶4) by gavage,respectively,and groups B and C were given NaCl (1 mL/100 g) and vermin,respectively.Groups were treated for a total of 8 weeks.After the treatment,the blood and gastric tissue samples of the rats were obtained,serum IL-1β and IL-6 levels were detected by ELISA.HE staining was used to observe the morphology of gastric tissue.RT-PCR detection of JAK2 mRNA,STAT3 mRNA,AKT1 mRNA,YAP1 mRNA,VEGF mRNA,EGFR mRNA expression in gastric tissue,the levels of p-STAT3,p-JAK2,p-AKT and p-YAP1 in gastric tissue were detected by WB.The experimental results found that the main active components of Coptidis Rhizoma and Pinelliae Rhizoma can bind to key targets; Each treatment group can effectively repair gastric mucosa morphology,reduce inflammation and intestinal metaplasia,and group E is better than other groups; The serum levels of IL-6 and IL-1β,the levels of JAK2 mRNA,STAT3 mRNA,AKT1 mRNA,YAP1 mRNA,VEGF mRNA,EGFR mRNA and the levels of p-STAT3,p-JAK2,and p-AKT1 in groups E and F were all in gastric tissue.It was significantly lower than that of group B and C (P<0.01).However,the level of p-YAP1 in each treatment group was significantly higher than that in group B (P<0.01),and group E was better than other groups.The experimental results show that the main components of Coptidis Rhizoma and Pinelliae Rhizoma can restore the morphology of gastric mucosa and prevent the evolution of malignant tumors by reducing the level of serum inflammatory factors in rats with chronic atrophic gastritis,inhibiting the JAK2/STAT3 pathway and angiogenesis pathway.
    Related Articles | Metrics | Comments0
    An study on the anti-rheumatoid arthritis effects of Chimonanthus zhejiangensis essential oil based on human mast cells
    LI Shu-hang, WANG Wen-cui, YAO Lei
    NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2022, 34 (增刊1): 33-38.   DOI: 10.16333/j.1001-6880.2022.S.005
    Abstract563)      PDF(pc) (1159KB)(16)       Save
    This study is aimed to explore the inhibitory effect of essential oil from Chimonanthus zhejiangensis on mast cell activation in rheumatoid arthritis and the efficacy of its main monomer.The essential oil was extracted by hydrodistillation,and gas chromatography-mass spectrometry (GC-MS) was used to analyze the volatile chemical compositions and determine the content of the main component.The cell toxicity of essential oil and the main component was tested by cell counting kit 8 (CCK-8).LAD2 was stimulated by C48/80.The degranulation and release of cytokines from LAD2 at different concentrations of essential oil and its main component were detected by linked immunosorbent assay (ELISA).The positive drug methotrexate was selected as the control.The results showed that the main component of essential oil from C. zhejiangensis was eucalyptol,and its content was 0.805 g/mL;essential oil and eucalyptol could inhibit mast cell degranulation at low,medium and high concentrations,and significantly reduced the release of cytokines including IL-4,IL-8,TNF-α and VEGF;the inhibitory effect of essential oil was better than that of eucalyptol at the same concentration,which was close to the effect of the positive drug.In a conclusion,both the essential oil from C. zhejiangensis and eucalyptol can inhibit the degranulation and inflammatory reaction of mast cells.It can be inferred that the essential oil from C. zhejiangensis has the effect on relieving rheumatoid arthritis,in which eucalyptol plays an important role as the main component.
    Related Articles | Metrics | Comments0
    Historical evolution and research status of processing of Cyperi Rhizoma
    CHEN Yi-long, LI Na, CHU Rui, LI Sheng-rong
    NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2023, 35 (4): 722-731.   DOI: 10.16333/j.1001-6880.2023.4.018
    Abstract536)      PDF(pc) (948KB)(5)       Save
    Cyperi Rhizoma,the rhizome of Cyperus rotundus L.,is commonly used in gynecology,which is considered to be the most important drug for regulating of painful menstruation.It is generally used after processing in clinic.In this paper,the processing methods of Cyperi Rhizoma in the Chinese Pharmacopoeia and the processing specifications of various provinces were summarized.As well as the efficacy of TCM theories and pharmacological effects of different processed products,and the basis of medicinal substances and mechanism of action after processing were summarized.The perfection and improvement of processing specifications of Cyperi Rhizoma,the discovery of other effective components and the exploration of action mechanism were prospected.It can provide reference for the in-depth study of the processing of Cyperi Rhizoma.
    Related Articles | Metrics | Comments0
    Research progress on chemical constituents,pharmacological mechanism,quality control and processing of Terminalia chebula Retz.
    LI Hua-shuang, LIU Yong-jian, YANG Hong-liu, LIU Yong-gang
    NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2022, 34 (12): 2130-2141.   DOI: 10.16333/j.1001-6880.2022.12.017
    Abstract528)      PDF(pc) (1375KB)(26)       Save
    Based on domestic and foreign literatures in recent years,this paper systematically gave a review on the advance of studies for chemical composition,pharmacological action and mechanism,quality control and processing of T. chebula.The main chemical components of T. chebula include tannins,phenolic acids and triterpenoids.Modern pharmacological studies have shown that T. chebula extract has many pharmacological activities,such as anti-microbial,detoxification,anti-cancer,anti-oxidation,protecting the brain,liver and gastrointestinal mucosa,detoxify aconite.In this paper,the chemical composition,pharmacological action and mechanism,quality standard,processing research and its own safety of T. chebula are systematically described,providing theoretical basis for scientific and rational use of T. chebula and further development and application of T. chebula.
    Related Articles | Metrics | Comments0
    Analysis of medicinal components in the flower of Hibiseu manihot L.
    WANG Rui-jun, TIAN Yu, WU Jia-wen, WANG Yan-feng, JIA Ze-han, BAI Zhen-qing, ZHANG Xiu-juan
    NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2022, 34 (4): 588-597.   DOI: 10.16333/j.1001-6880.2022.4.006
    Abstract519)      PDF(pc) (2302KB)(15)       Save
    The flower of Hibiseu manihot L. has rich flavonoid compositions which have high utilization value in food,medicine and health care.To compare the chemical composition differences in the flowers of H. manihot with different medicinal methods and determine the best medicinal period of H. manihot This experiment uses ultra-high performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS) analyzed the complete components of dried flowers,buds and fresh flowers of H. manihot.A total of 141 compounds were identified.Some of the flavonoids and organic acid compounds in the fresh flowers were higher than those in dried flowers and buds.In addition,fresh flowers and dried flowers have more medicinal ingredients and higher content than buds.The results showed that there are differences in the chemical composition of the flowers in different ways of medicine,and it should be picked and used according to the needs.All in all,this research provides a theoretical basis for the rational development and utilization of H. manihot.
    Related Articles | Metrics | Comments0
    Appearance characters and internal quality of cultivated Fritillaria cirrhosa D.Don under different drying methods 
    LI Qiao, CHONG Ye-min, CHEN Ying-xin, CAI Xiao-yang, LI Min, HUANG Yong
    NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2022, 34 (6): 916-924.   DOI: 10.16333/j.1001-6880.2022.6.002
    Abstract508)      PDF(pc) (1303KB)(33)       Save
    To investigate the effects of different drying methods on the appearance and internal quality of cultivated Fritillaria cirrhosa D.Don (CFC) by using intuitive analysis, cluster analysis (HCA) and principal component analysis (PCA). Results indicated that traditional sun-dried CFC has better appearance, white surface color, enough cross section mealiness, and high content of effective ingredients, but the drying rate was slow and vulnerable to the environment impact, while the drying rate of hot air drying was faster and the time was shortened. When the temperature was 50-55 ℃, the appearance of CFC was similar to that of the sun drying method, except for the content of total starch, the extract and total alkaloids all increased. However, in the fixation treatment and the 60 ℃ treatment, the cross section mealiness was weakened, degree of keratinization was increased, which also promoted the decomposition of starch, the starch content was reduced by 33.6% and 26.25% compared with the traditional drying method. The surface color of freeze-dried medicinal material was off-white, and the internal texture was loose and porous, and the texture became lighter, the content of total alkaloids and total starch was highest (0.191% and 70.37%, respectively). Comprehensive analysis showed that 50-55 ℃ hot air drying method are better than sun drying method, and can be popularized and applied in the processing of CFC in production place; in the freeze-drying method, the content of active ingredients is high, and it can be used for extraction of medicinal effective ingredients.
    Related Articles | Metrics | Comments0
    Study on the antioxidant activity of fermented and aqueous-extracted Pueraria lobata and their effect on delaying Caenorhabditis elegans aging
    ZHAO Dan, WU Di, LI Meng, WANG Chang-tao, ZHANG Jia-chan, WANG Dong-dong
    NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2022, 34 (4): 639-646.   DOI: 10.16333/j.1001-6880.2022.4.012
    Abstract501)      PDF(pc) (1071KB)(36)       Save
    To explore the application of Pueraria lobata in antioxidant and anti-aging field,two methods of fermentation and aqueous extraction were used to extract the active substances of P.lobata.The biochemical experiments were detected to evaluate the antioxidant activity of the extracts and Caenorhabditis elegans were used as model animal to detect the anti-aging effect.Results showed that 6.8% fermented broth of P. lobata (FBP) had better antioxidant ability than aqueous extract of P. lobata (AEP).The antioxidant ability included the total antioxidant capacity,the scavenging capacity of hydroxyl free radicals and superoxide anions.Compared with control group,FBP could significantly prolong the lifespan of C .elegans (P<0.05),and promote the fecundity of C. elegans by 4.8%.At the same time,the heat-resistant time of C. elegans was increased to 111.6% of the control group.Real-time quantitative PCR results showed that FBP could inhibit the expression of the pro-aging gene daf-2 and up-regulate the expression of the anti-aging gene daf-16.In addition,the FBP promote the expression of the downstream heat-resistant genehsp-16.2 and the antioxidant gene of sod-3.In conclusion,the FBP could prolong the lifespan of C. elegans by improving the antioxidant activity and heat resistance of the C. elegans.The good antioxidant and anti-aging effect of FBP provide a reference for its application in the field of anti-aging related food and cosmetics.
    Related Articles | Metrics | Comments0
    Optimization of extraction process and composition analysis of essential oil from Amomum tsao-ko
    LIU Ju-zhao, XIAN Meng-xue, KONG Wei-hua, TIAN Xiao-li, YUAN Qiang, CUI Qi
    NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2023, 35 (5): 766-780.   DOI: 10.16333/j.1001-6880.2023.5.005
    Abstract496)      PDF(pc) (2439KB)(7)       Save
    In order to study the optimal extraction technology of essential oil from Amomum tsao-ko by steam distillation and ultrasonic organic solvent extraction.In this paper,based on single factor experiment,the extraction process of A. tsao-ko essential oil by steam distillation and ultrasonic organic solvent extraction was optimized by response surface methodology (RSM) and back-propagation artificial neural network (BP-ANN).Gas chromatography-mass spectrometry (GC-MS) was applied to analyze the essential oil components obtained from the two extraction methods,and the models were compared.The results showed that the optimum technological parameters of A. tsao-ko essential oil extraction by steam distillation were soaking time of 53.00 min,distillation time of 72.00 min,liquid to solid ratio of 11.00 mL/g,and extraction yield of 1.59%.A total of thirty-four components were identified in A. tsao-ko essential oil obtained by steam distillation.Under the optimum conditions of ultrasonic organic solvent extraction with ultrasonic power of 150.00 W,ultrasonic time of 27.00 min and liquid to solid ratio of 6.30 mL/g,the extraction yield was 3.40% and a total of 43 components were identified.Although the extraction yield of A. tsao-ko essential oil by ultrasonic organic solvent extraction was higher than that by steam distillation,the essential oil obtained by ultrasonic organic solvent extraction was poor in quality,dark in color and heavy in impurities,which was not conducive to the subsequent application.Compared with the RSM model,the BP-ANN model had a lower error-index and a higher fitting degree in predicting extraction yield,and had higher accuracy and prediction advantages.This study will provide reliable technological parameters for the efficient extraction of A. tsao-ko essential oil and further promote the development and utilization of the medicinal and edible plant A. tsao-ko.
    Related Articles | Metrics | Comments0
    Comparative study of chemical components in different organs of Macleaya cordata by HPLC fingerprint and chemometrics
    YANG Min, LIU Xiao-rong, ZENG Mei, ZHOU Xin-ru, WANG Zhi-hui, TONG Qiao-zhen, ZHOU Ri-bao, LIU Xiang-dan
    NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2022, 34 (7): 1098-1109.   DOI: 10.16333/j.1001-6880.2022.7.002
    Abstract493)      PDF(pc) (2919KB)(13)       Save
    In this study,the chemical fingerprints of roots,leaves,fruits and stems of Macleaya cordata were established by HPLC,and the contents of protopine,allocryptine,sanguinarine and chelerythrine were determined;orthogonal partial least squares discriminant analysis (OPLS-DA) was used to evaluate the quality of roots,leaves and fruits of M. cordata.The fingerprint results showed that 17 batches of root samples were calibrated with 10 common peaks,14 batches of leaf samples were calibrated with 9 common peaks,14 batches of fruit samples were calibrated with 9 common peaks,and 10 batches of stem samples were calibrated with 6 common peaks.The order of the total contents of four alkaloids in different organs of M. cordata is:fruit > root > leaf > stem;the contents of the above four alkaloids were higher in the fruit and were lower in the stem;the contents of protopine and allocryptine higher in the root and leaf.The resources of M. cordata roots are large,and the contents of protopine and allocryptopine are higher than those of fruits and leaves,it can be developed and utilized with M. cordata fruits and leaves as the above alkaloid extraction raw materials.The results of OPLS-DA showed that if VIP > 1,allocriptine and chelerythrine could be used as potential quality markers of M. cordata roots;sanguinarine and chelerythrine can be used as potential quality markers of M. cordata leaves;protopine,allocryptine and sanguinarine can be used as potential quality markers of M. cordata fruits.The results of this study provide a research basis for the identification and evaluation of the roots,leaves,fruits and stems of  M. cordata and the development and utilization of M. cordata roots.
    Related Articles | Metrics | Comments0
    Study on the enzyme-assisted extraction process of essential oil from Ledum palustre L. and its antioxidant activity
    KANG He-di, ZHAO Xiu-hua, ZHANG Xiao-xue, ZHANG Qian, GENG Yu-ting, ZHAO Wen
    NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2022, 34 (10): 1678-1689.   DOI: 10.16333/j.1001-6880.2022.10.005
    Abstract492)      PDF(pc) (1908KB)(15)       Save
    To explore a green, efficient and low-energy method for extracting essential oil from Ledum palustre L., the enzyme-assisted water vapor distillation method was used to extract essential oil, and the chemical composition was analyzed and compared with the conventional water vapor distillation method by gas chromatography-mass spectrometry, and the antioxidant activity of essential oil was studied as well. The results showed that the optimal process conditions of the enzyme-assisted water vapor distillation method are as follows: the material-to-liquid ratio is 1∶20 g/mL, the pH of the enzyme is 5.5,the enzymolysis time is 4 h, the enzymolysis temperature is 55 ℃, the concentration of the enzyme is 1.5%, and the extraction time is 1.5 h. Under this condition, the extraction rate of essential oil from L. palustre is 1.71%. Compared with water vapor distillation, the extraction rate of essential oil increased by 67.65%. There are 53 kinds of chemical components of essential oil from L. palustre obtained by enzyme-assisted extraction, and only 44 kinds of chemical components of essential oils extracted by water vapor distillation method. It is shown that enzymolysis can make the essential oil better released, so that the chemical composition of the enzyme-assisted extraction of essential oil is abundant. In the antioxidant experiment, the essential oil extracted by enzyme assisted extraction had stronger scavenging ability on DPPH free radical, ABTS free radical and hydroxyl free radical than the essential oil extracted by steam distillation. In general, the essential oil extracted by enzyme assisted extraction had obvious antioxidant activity and a good dose-response relationship. This study will provide reliable process parameters for the large-scale production of essential oil, and provide basic data and reference for evaluating its antioxidation.
    Related Articles | Metrics | Comments0
    Quality evaluation and preliminary antioxidant activity of Paeonia ludlowii seeds
    QIN Wei-han, LAN Xiao-zhong, QU Xian-you, QIAO Xin-fang, LIU Xiang, XU Yuan-jiang, ZHOU Jia-jia, HE Dan
    NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2022, 34 (11): 1919-1929.   DOI: 10.16333/j.1001-6880.2022.11.013
    Abstract491)      PDF(pc) (1241KB)(8)       Save
    A method for quality evaluation of Paeonia ludlowii seeds was established by combining QAMS and fingerprints,and its antioxidant activity was preliminarily evaluated.The analyses were performed on Agilent DB-FATWAX column (0.25 mm×30 m,0.25 μm),the carrier gas was high-purity nitrogen,temperature was programmed,and hydrogen flame ionization detector was used.Using methyl oleate as internal reference,the relative correction factor has good reproducibility.The P values of QAMS and external standard method are both >0.05 by t-test.The similarity between the fingerprints of P. ludlowii samples from different habitats and the control fingerprints was >0.99;The results showed that QAMS and fingerprints could be used for quality evaluation of P. ludlowii  seeds.The results of principal component analysis (PCA) showed that the data points of P. ludlowii  and P. suffruticosa could be divided into two categories.Through the comparative analysis of DPPH and Fe3+ reduction methods,it was found that the free radical scavenging rate and Fe3+ reduction capacity of the seed coat of P. ludlowii  were higher than P. suffruticosa,while the antioxidant capacity of seed oil was not significantly different with  P. suffruticosa.The analytical method established in this study is accurate,simple and feasible.One test and multiple evaluation combined with fingerprint can effectively evaluate the quality of P. ludlowii  seeds from different habitats.The seed coat and kernel of P. ludlowii  have obviously antioxidant effect,and can be used for research and development of new products such as food and cosmetics.
    Related Articles | Metrics | Comments0
    Compounds from Murraya tetramera Huang and their cytotoxic activity (Ⅲ)
    ZHOU Yong-fu, YAO Ru-jie, LI Xue-ming, YANG Cheng-wei
    NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2023, 35 (4): 622-629.   DOI: 10.16333/j.1001-6880.2023.4.009
    Abstract491)      PDF(pc) (638KB)(10)       Save
    In order to study the pharmacodynamic material basis of the Murraya tetramera Huang;in this paper,fifteen compounds were isolated and purified from the ethyl acetate extract of its alcohol extract by modern chromatographic separation technology.And Identified the structure of the compounds by NMR spectroscopy,which were luteolin-7-O-glucoside(1),3,4-dihydroxy benzeneacrylic acid(2),2-hydroxylcinnamie acid(3),3,8-dihydroxy-4-methoxy-coumarin(4),2-methyl-6-hydroxychromone(5),chlorogenic acid(6),methyl caffeate(7),furo[3,2-α]carbazole(8),(E)-2-((4-hydroxyphenyl)diazenyl)phenol(9),N-(2,6-dimethylphenyl)-N′-(4′-nitrophenyl)urea(10),murrayanine(11),phorbazole C(12),3-(2’-hydroxyphenyl)-4(3H)-quinazolidone(13),N-trans-feruloyl-3-methyldopamine(14),6-acetone-5,6-dihydrosanguinarine(15). All compounds are isolated from this plant for the first time.The tumor inhibitory and proliferation effects of 15 compounds were analyzed by the MTS method.The inhibitive effects of the compounds on the growth of five tumor cells in vitro were studied.Especially,the compound 1,8-15 have good growth inhibitory effect on human tumor cell lines HL-60,A-549,SMMC-7721,SW-480 and MCF-7.
    Related Articles | Metrics | Comments0
    Research progress on chemical constituents,pharmacological activities and clinical application of Euphorbia kansui
    MA Li, MENG Xian-hua, YANG Jun-li
    NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2022, 34 (4): 699-712.   DOI: 10.16333/j.1001-6880.2022.4.018
    Abstract470)      PDF(pc) (1899KB)(35)       Save
    The dry root tuber of Euphorbia kansui T.N.Liou ex T.P.Wang(Euphorbia genus in Euphorbiaceae) is a unique Chinese herbal medicine in China.The main chemical constituents and active components of E. kansui are jatrophane diterpenes,ingenane diterpenes,euphane triterpenes and tirucallane triterpenes,which have many pharmacological activities such as anti-tumor,anti-virus,anti-inflammatory,insecticidal,antioxidant and immune system inhibition.This review comprehensively summarized 102 terpenes and 14 steroids and their structural characteristics,and systematically summarized the pharmacological activity,processing and clinical application of  E. kansui,in order to provide reference for clinical rational drug use,quality control,resource comprehensive utilization and development of  E. kansui.
    Related Articles | Metrics | Comments0
    Study on the function of cathelicidin-DM in Duttaphrynus melanostictus
    ZHANG Kang, WANG Gui-xi, ZHANG Jin-yang, HAN Qin-qin, XIA Xue-shan, SONG Yu-zhu
    NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2022, 34 (增刊1): 104-108.   DOI: 10.16333/j.1001-6880.2022.S.016
    Abstract465)      PDF(pc) (516KB)(26)       Save
    The broad-spectrum antibacterial activity of cathelicidin-DM has been proven,and this study intends to explore its other biological activities to further promote its clinical application.cathelicidin-DM was synthesized by solid-phase synthesis,and the antioxidant activity,hemolytic activity,lectin activity,cytotoxicity and tumor cytotoxicity were detected.The results show that cathelicidin-DM has strong scavenging activity for ABTS+ free radicals,but relatively weak scavenging activity for DPPH free radicals,and has certain cytotoxicity to the RAW 264.7.Hemolytic activity,lectin activity,and tumor cell toxicity are not obvious.cathelicidin-DM not only has broad-spectrum antibacterial activity,but also has antioxidant activity.Solving its cytotoxicity will be a major pre-clinical challenge.Or it can be used as a biological antioxidant.
    Related Articles | Metrics | Comments0
    The mechanism of Astragali Radix-Cordyceps on the treatment of IgA nephropathy based on network pharmacology, molecular docking and rat experiments 
    FU Yi, CHEN Bang-ming, LI Xin, FU Yi, WU Hong-ze, LIU Yong-fang
    NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2022, 34 (9): 1573-1581.   DOI: 10.16333/j.1001-6880.2022.9.015
    Abstract460)      PDF(pc) (1588KB)(48)       Save
    To screen the targets and related signaling pathways of Astragali Radix-Cordyceps in the treatment of IgA nephropathy (IgAN) by network pharmacology,to clarify its mechanism of action,and to verify it experimentally.TCMSP and BATMAN-TCM databases combined with literature mining were used to obtain the active components and targets of Astragali Radix-Cordyceps;the disease targets of chronic IgAN were obtained through GeneCards and OMIM databases;Venny 2.1 was used to draw a Venn diagram of common targets;STRING was used to build a common target interaction network (PPI);Cytoscape 3.7.1 software was used to build an interaction network of Astragali Radix-Cordyceps pills-target;through R language software GO analysis of common targets and KEGG analysis of chronic IgA nephropathy targets were performed to screen out potential pathways and their mechanisms of action were analyzed.Molecular docking technology was used to verify the binding efficacy of the active components of Astragali Radix-Cordyceps and key targets.IgA nephropathy model rats (model group and Astragali Radix-Cordyceps dose group with high,medium and low doses) were taken for 21 days,respectively,and the kidney tissue was collected to detect the expression of VEGFA protein in the kidney tissue of the rats in each group by Western blot.Five bioactive components were screened out from Astragali Radix-Cordyceps,which acted on 37 common targets of IgAN.The core targets were VEGFA,HIF1A,NOS3,and CASP3,which mainly involved cysteine-type steroid binding and apoptosis,endopeptidase activity,estrogen receptor binding,cholesterol binding and other biological processes are mainly enriched in lipid and atherosclerosis signaling pathway,AGE-RAGE signaling pathway,fluid shear stress and atherosclerosis pathway,PI3K-Akt signaling pathway and other signaling pathways.The molecules showed good binding potency between the main components and key targets.Compared with model group,the expression of VEGFA in the high and low dose groups of Astragali Radix-Cordyceps decreased (P<0.05),and there was no significant difference between the high-dose group and the middle-dose group (P> 0.05).Astragali Radix-Cordyceps may act on key targets such as VEGFA,HIF1A,NOS3,and CASP3,and achieve therapeutic effects on IgA nephropathy through fibrosis and other signaling pathways.
    Related Articles | Metrics | Comments0