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    Chemical Constituents of the Bark of Anthocephalus chinensis
    XU Xiao-yu, LI Shang-zhen, SONG Qi-shi
    NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2011, 23 (3): 393-398.  
    Abstract5031)      PDF       Save

    Ten compounds were isolated and purified from the bark of Anthocephalus chinensis by column chromatography on silica gel, MCI and Sephadex LH-20, and their structures were elucidated as 4-carboxy-3-hydroxy-5-methylphenyl 3-methoxy-4-hydroxy-5-methylbenzoate (1), sitosterol-3-O-(6'-O-palmityl)-β-D-glucoside (2), quinovic acid-3-O-α-L-rhamnopyranoside (3), clethric acid (4), hederagenin (5), uncargenin C (6), morolic acid (7), caffeic acid methyl ester (8), cadambine (9), and 3α-dihydrocadambine (10) by modern spectroscopic methods. Compound 1 was a new phenolic compound, and compounds 2-8 were obtained from Anthocephalus plantsfor the first time.

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    Antioxidant Activity of Flavonoids from Branches Leaves and Berry of Hippophae rhamnoides Linn
    JIAO Yang1,LI Cai-xia1,GAO Hai-ning1,FENG Jiu-hai2*
    NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2015, 27 (4): 655-660.   DOI: 10.16333/j.1001-6880.2015.04.020
    Abstract1601)      PDF(pc) (695KB)(1242)       Save
    Using flavonoids from Hippophae rhamnoides L.berry (ZYCFJ) as reference,the antioxidant activities of flavonoids in branches and leaves of Hippophae rhamnoides L.from Zhangye (ZYCF) and Xinjiang (XJCF) were evaluated by measuring their scavenging activity against DPPH,ABTS,hydroxyl,superoxide anion radicals and reducing Fe3+ and inhibiting nitroso.The results showed that at 50 μg/mL concentration level,the clearance rates of ZYCFJ,ZYCF and XJCF on ABTS+ were 60.8%,42.6%,45.4%,and on DPPH were 95.7%,80.6%.88.6%.When flavonoids concentrations higher than 40 μg/mL,the scavenging activity against DPPH and ABTS+ of ZYCFJ was better than XJCF and ZYCF.At 500 μg/mL concentration level,the scavenging rates of ZYCFJ,ZYCF and XJCF on ·OH were 92.7%, 88.9%, 98.6%,and on O2- were 76.5%,70.6%,82.4%,and inhibition ratio of ZYCFJ,ZYCF and XJCF on nitrite were 98.7%,76.8%,83.7%.In term of the inhibition rates of nitrite and the reduction of Fe3+,ZYCFJ was better than that of ZYCF and XJCF.The scavenging effect of ZYCFJ,ZYCF and XJCF on superoxide anion and hydroxyl free radical were equivalent.
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    Screening and Identification of Pectinase Producing Strain and Study on Its Pectinaseproducing Conditions
    YUAN Zhi-hui*
    NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2013, 25 (12): 1627-1630.  
    Abstract1047)      PDF(pc) (476KB)(1052)       Save
    The aim of this work was to screen highly pectinase producing bacteria for following laboratorial research and industrial application.The soil sample that is rich in pectin substance was collected from ramie field located in the suburbs of LingLing district for screening of pectinase producing bacteria,and 11 stains were gained by enrichment procedure and screening test successively.Strain HYL6 which produced the highest enzyme activity tested by 3,5dinitrosalicylic acid (DNS) method in 11 strains was identified as Bacillus sp.and its pectinase producing conditions were optimized by onefactor experiments and orthogonal test successively.The optimized conditions were as follows:soluble starch 5 g/L,peptone 15 g/L,pH 7,and shaking fermentation 34 hours at 36 ℃,180 rpm.The activity of pectinase produced by the strain HYL6 under the optimal conditions reached 415 U/mL.
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    Identification and Quantification of Phenolic Components of Erigeron breviscapus and Its Derived Drug Breviscapine
    ZHANG Ren-wei 1*,FAN Xian-er1,LI Lai-wei1,LIN Long-ze2,HARNLY James M2
    NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2015, 27 (6): 962-970.   DOI: 10.16333/j.1001-6880.2015.06.005
    Abstract1254)      PDF(pc) (260KB)(878)       Save
    A standardized HPLC-PDA-ESI/MS profiling method was used to identify the phenolic components of Erigeron breviscapus and breviscapine. A general phenolic quantification method was used to quantify the components. As a result,33 hydroxycinnamates and 16 flavonoids were detected in E. breviscapus, a plant used in traditional Chinese medicine for treating cardiocerebral diseases. Eighteen flavonoids were detected in breviscapine,an E. breviscapus derived drug. The hydroxycinnamates were quantified using HPLC-PDA at the detection wavelength of 326 nm and the mole relative response factors (MRRF). Dried chlorogenic acid was used as calibration standard. Similarly,8 apigenins,4 luteolins,3 flavonol 3-O-glycosides,and the 7-O-glucuronides of quercetin and isorhamnetin were quantified using apigenin (at 336 nm) and rutin (at 354 nm) as calibration standards and MRRF value for each compound. This was the first detection of over 10 flavonoids in breviscapine,and the quantification of each phenolic component in E. breviscapus.
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    Advance on Chemical Compositions and Pharmacological Studies of Semiaquilegia adoxoides
    XU Ran, XIAO Hai-tao, WANG Jian-ta, TANG Lei*
    NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2014, 26 (7): 1154-1159.  
    Abstract1552)      PDF(pc) (674KB)(815)       Save
    The chemical constituents,pharmacological and clinical effects of the Semiaquilegia adoxoides were reviewed in orde to provide a reference for future research and development of the genus.
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    The Extraction Technology of Cannabidiol in Hemp Leaves
    GAO Bao-chang*, SUN Yu-feng, ZHANG Xu, TIAN Yuan, SHI Yu, CUI Bao-yu
    NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2018, 30 (增刊1): 153-156.   DOI: 10.16333/j.1001-6880.2018.S.028
    Abstract548)      PDF(pc) (692KB)(791)       Save
    leaves.The optimized conditions were extract with ethyl alcohol,ultrasound-assisted oscillate for 15 minutes,ratio of sample to solvent 1∶20,extraction 3 times.Meanwhile,the purification process of cannabidiol was also studied.The adsorption and elution capacity of cannabidiol by macroporous adsorption resin were investigated.The optimized macroporous adsorption resin is DM130.The product was characterized by HPLC,LCMS and HNMR.Finally,the cannabidiol of extracting and purification was established.
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    Prodrug Decision of Novel Genistein Sulfonate and Its Bioavailability in Rats
    PENG You, LIU Xin-qiang, DENG Ze-yuan
    NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2012, 23 (1): 35-41.   DOI: A
    Abstract1883)      PDF(pc) (221KB)(762)       Save
    The oral bioavailability of genistein (GE) in its benzensulfonate prodrug was studied in search for new drug or food functional ingredient. The plasma were collected at different points of time after the intragastric or intravenous administration of genistein benzensulfonate (GB) 40 mg/kg to rats. The GB and GE contents in plasma were determined by high performance liquid chromatography (HPLC). The compartment model was fitted and pharma cokinetic parameters were calculated by DAS 2.1.1. The results indicated that the dynamic processes of GE was consistent with two compartment model after intragastric or intravenous administration of GB prodrug to rats. The relative oral bioavailability of GE in prodrug GB was 110.9%. In conclusion, the above results demonstrated that the oral bioavailability of GE in prodrug had been improved remarkably.
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    Cladosperanol A,a New Dimeric Tetralone from Marine-derived Fungus Cladosporium perangustum FS62
    FAN Zhen, SUN Zhang-hua, CHEN Yu-chan, LI Hao-hua, ZHANG Wei-min
    NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2016, 28 (4): 487-489.  
    Abstract894)      PDF(pc) (585KB)(747)       Save
    A new compound (1) was isolated from the fermentation broth extract of marine fungal strain Cladosporium perangustum FS62 by various chromatographic methods such as silica gel,reverse silica gel,Sephadex LH20,HPLC, etc. On the basis of detailed spectroscopic analysis (HR-ESI-MS,1D NMR,2D NMR,CD),1 was elucidated as a new dimeric tetralone with the structure of (4S,4′S)-4,8,8(trihydroxy-2,3,2′,3′- tetrahydro-[4′,5-binaphthalene]-1,1′(4H,4′H)-dione,named as cladosperanol A.
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    A New DrimaneSesquiterpenoid from Cultures of Fungus Psathyrellacandolleana
    LI Xue-mei1,2, YIN Xia1,3, LI Zheng-hui1, FENG Tao1*, LIU Ji-kai1*
    NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2015, 27 (7): 1131-1133.   DOI: 10.16333/j.1001-6880.2015.07.001
    Abstract1483)      PDF(pc) (136KB)(729)       Save
    A new drimanesesquiterpenoid15-hydroxydrimenol (1), together with a known germacranesesquiterpenoid1α-hydroxy-bisabola-2,10-dien-4-one (2),was isolated from cultures of fungus Psathyrellacandolleana. The new compound 1 was elucidated on the basis of extensive spectroscopic methods,while the known compound 2 was identified by comparison of spectroscopic data with those reported in the literature.
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    Compounds from Lycium ruthenicum
    QI Jing-jing1,2, YAN Yong-ming3, WANG Cheng-xiang4, CHENG Yong-xian1,2,3*
    NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2018, 30 (3): 345-353.   DOI: 10.16333/j.1001-6880.2018.3.001
    Abstract1142)      PDF(pc) (817KB)(716)       Save
    Twenty two compounds were isolated from the fruits of Lycium ruthenicum Murr. Their structures were identified as 4-p-cumaroyl-α-L-rhamnosyl-(1→6)-β-D-glucose(1),4-p-cumaroyl-α-L-rhamnosyl-(1→6)-α-D-glucose (2),isosalipurposide (3),ethyl caffeate (4),trans-ferulic acid (5),quercetin 3-O-β-D-rutinoside (6),kaempferol-3-O-rutinoside (7),isorhamnetin 3-rutinoside (8),kaempferol 3-rutinoside-4′-glucoside (9),naringenin 7-O-β--D-glucopyranoside (10),ampelopsin 4′-O-β--D-glucopyranoside (11),helichrysin A (12),naringenin (13),quercetin (14),scopoletin (15),evofolin B (16),(+)-isolariciresinol (17),methyl-2,4-dihydroxy-5-methoxybenzoate (18),3-O-methylgallic acid (19),4-acetyl-2,6-dimethoxyphenol (20),acetovanillone (21) and methyl benzoate (22),respectively,by spectroscopic methods.Compound 1 is a new substance.In addition,compounds 2,3,9,11,12,and 16-19 were isolated from the genus Lycium for the first time.
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    Anti-inflammatory Activity of Forsythia suspensa Extract on Human Airway Epithelial Cells Inflammation Model
    LIU Jian-zhou,ZHANG Li-wei*
    NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2015, 27 (7): 1248-1253.   DOI: 10.16333/j.1001-6880.2015.07.023
    Abstract1294)      PDF(pc) (945KB)(706)       Save
    To investigate the anti-inflammatory activities of Forsythia suspensa extract (FSE) with emphasis on excretion of inflammatory cytokines by LPS-induced BEAS-2B inflammation model.BEAS-2B cell viability was detected by MTT method in order to screen out the optimal drug dose of F.suspensa ethanol extracts (FSEE),60% forsythiaside A (60% FTA) extracts and 90% forsythiaside A (90% FTA) extracts;The morphology of BEAS-2B cells treated with FSE were observed by microscope; NO content of cell culture supernatant was determined by Griess Reagent method;Intracellular reactive oxygen species (ROS) was analyzed by flow cytometry.Toxical experimental results revealed that FSE did not show toxic reaction to BEAS-2B cell at concentration of less than 128 μg/mL;while both 60% FTA and 90% FTA showed toxicity at the concentration between 128 and 256 μg/mL.Anti-inflammatory results showed that all FSE can inhibit the secretion of NO (P<0.01) at concentration 25,50,and 100 μg/mL,and 90% FTA extracts exhibited a dose-dependent manner;The elevated level of intracellular ROS in LPSstimulated BEAS-2B cells was significantly diminished by all FSE in a dose-effect manner (P<0.05).In addition,the therapeutic effect of FSEE,60% FTA and 90% FTA increased gradually.It was concluded that the 90% FTA extracts were the main active fraction of anti-inflammatory effect and forsythiaside A was possibly the active ingredient.The results presented in this study will be helpful for the further study of F.suspensa on anti-inflammatory activity.
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    Chemical Constituents from Ganoderma applanatum and Absolute Configuration Determination of New Compounds
    BU Qing-hua1,2#,LUO Qi2#,CHENG Yong-xian2*
    NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2016, 28 (5): 650-654.   DOI: 10.16333/j.1001-6880.2016.5.002
    Abstract1318)      PDF(pc) (540KB)(687)       Save
    Eight compounds including two new compounds,namely shushenes A (1) and B (2),were isolated from the fruiting bodies of Ganoderma applanatum. Their structures were identified by means of spectroscopic methods.The structures of the known compounds are lucidulactone A (3),2,3-dihydroxy-1-(4-hydroxy3,5-dimethoxyphenyl)-1-propanone (4),6-hydroxy-4-H-chromen-4-one (5),1-(4-ethylphenyl)-1,2-ethanediol (6),1-(2-ethylphenyl)-1,2-ethanediol (7) and indole-3-carboxaldehyde (8),which were all isolated from this species for the first time.Of note,compounds 1 and 2 were isolated as racemic mixtures,chiral separation followed by computational methods allowed their absolute configuration assignment.In addition,inhibition of compounds 1 and 2 on fibronectin expression was tested by using an enzyme-linked immunosorbent assay (ELISA).
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    Chemical Constituents from the Fruits of Capparis masaikai
    LIAO Jin-hua1, HU Xu-jia1*, YUAN Chun-mao2, HE Hong-ping2, DI Ying-tong2*
    NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2014, 26 (11): 1780-1784.  
    Abstract1944)      PDF(pc) (207KB)(681)       Save
    Ten compounds were isolated and purified by silica gel column chromatography and Sephadex LH-20 from the fruits of Capparis masaikai.Their structures were identified as medioresinol (1), erythro-guaiacylglycerol-β-O-4′-sinapyl ether (2),hedyotol C (3),hedyotisol A (4),hedyotisol B (5),ozoroalide (6),5α,6α-epoxy-3β-hydroxyergosta-22-ene-7-one (7),coniferyl aldehyde (8),3-hydroxy-5-(p-hydroxyphenyl) pentanoic acid (9) and β-hydroxypropiovanillone (10) by physicochemical properties and spectral data.The structural types of isolated compounds include lignans,macrocyclic lactone,sterols and phenols.Compounds 1-10 were isolated from this plant for the first time,among of them,compounds 1-7 were firstly reported from the genus Capparis.
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    Chemical Constituents of Scrophularia ningpoensis Hemsl.
    LI Yuan, SONG Bao-an, YANG Song, HU De-yu, JIN Lin-hong
    NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2012, 23 (1): 47-51.   DOI: A
    Abstract2688)      PDF(pc) (401KB)(673)       Save
    Fourteen compounds were isolated from the ethanol extract of Chinese herbal medicine Scrophularia ningpoensis Hemsl., which were identified as sugiol (1), β-sitosterol (2), lupaneol (3), ursolic acid (4), Rhamnose (5), daucosterol (6), sucrose (7), caffeic acid (8), oleic acid (9), 3-O-(6′-O-palmitoyl-β-D-glucosyl)-spinasta-7,22-diene (10), clematomanshurica saponin E (11), α-caryphy (12), α-cedrol (13), and scrophuloside A4 (14) on the basis of physicochemical and spectroscopic evidences. Compounds 3, 5, and 8-14 were isolated from this plant for the first time.
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    Study on Transformation Rule of Saikosaponin a and Saikosaponin d Under Different Extraction Conditions by UPLC-QTof-MS
    GUO Zhi1, PENG Bing2, LI Zhong-yang1, PAN Rui-le1*
    NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2014, 26 (5): 716-720.  
    Abstract1262)      PDF(pc) (506KB)(646)       Save
    The transformation rules of saikosaponin a and saikosaponin d under different extraction methods were studied by ultra-performance liquid chromatography coupled with Quadrupole-time of flight mass spectrometry (UPLC-QTof-MS).A new UPLC-QTof-MS method was developed for profiling and quantification of the transformation products of saikosaponin a and saikosaponin d under different extraction methods.Chromatographic separation was performed on an Acquity UPLC BEH C18 column (2.1 mm×50 mm,1.7 μm) with a mobile phase consisting of acetonitrile (A) and 0.01% formic acid in water (B).The flow rate was kept constant at 0.45 mL/min with a gradient elution.QTof-MS was used as the detector.Twelve samples of Radix Bupleuri were individually extracted using 12 different extraction methods and were then analyzed by UPLC-QTof-MS with negative ion mode.The transformation rule of saikosaponin a and saikosaponin d under different extraction methods were derived:saikosaponin a and saikosaponin d were hydrolyzed into saikosaponin b1 and saikosaponin b2 under acidic condition at at room temperature.The glycosidic bond was hydrolyzed yielded to sapogenin under acid condition and being heated.Saikosaponin a and saikosaponin d were stable under neutral and alkaline condition.The experimental results provided a refrence for extraction,separation and analysis of saikosaponin a and saikosaponin d.
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    Secondary Metabolites of Endophytic Fungus Acremonium implicatum and Their Biological Activities
    CAO Yu-heng1,ZHANG Zi-hao1,LIN Feng-ke1,LAI Dao-wan1,YAO Yu-rong2,XIE Bing-yan2*,ZHOU Li-gang1*
    NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2016, 28 (2): 182-187.   DOI: 10.16333/j.1001-6880.2016.2.003
    Abstract1282)      PDF(pc) (196KB)(640)       Save
    Five secondary metabolites were isolated from the ethyl acetate extract of the endophytic fungus Acremonium implicatum from tomato root galls infected with Meloidogyne incognita. They were identified as 4-hydroxy benzaldehyde (1),4-hydroxy benzoic acid (2),indole-3-carboxaldehyde (3),5′-O-acetylthymidine (4) and cerevisterol (5) by physicochemical properties and spectrometric methods. Compounds 1 and 2 showed strong antibacterial,antifungal,antioxidant and antinematodal activities. Compound 3 exhibited moderate antinematodal activity on Caenorhabditis elegans and M. incognita with the median inhibitory concentration (IC50) values of 82.3 μg/mL and 93.4 μg/mL,respectively. The results indicated that 4-hydroxy benzaldehyde (1),4-hydroxy benzoic acid (2),and indole-3-carboxaldehyde (3) could be the main bioactive components in the endophytic fungus A. implicatum. The results will be helpful for clarifying biocontrol mechanisms of A. implicatum on the root-knot nematode as well as for development and utilization of this fungus. All the compounds were isolated from A. implicatum for the first time.
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    Three New Triterpenoids from Ganoderma petchii
    GUO Ping-xia, WANG Xin-long, CHENG Yong-xian, PEI Miao-rong
    NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2016, 28 (1): 2-15.  
    Abstract985)      PDF(pc) (801KB)(638)       Save
    Three new triterpenoids,petchinoids A-C (1-3),along with two known phenolic compounds 2,5-dihydroxyacetophenone (4) and grevillone (5),were isolated from the fruiting bodies of Ganoderma petchii. Their structures were identified as by means of spectroscopic methods.
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    Extraction and Purification of Ginkgo biloba Flavonoids Using [C8 mim][N(CN)2]/(NH4)2SO4 Aqueous Two-Phase System
    XU Xue-lei, LI Fen-fang*, TAN Zhi-jian
    NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2014, 26 (6): 830-833.  
    Abstract1238)      PDF(pc) (415KB)(637)       Save
    A new method for the extraction and purification of Ginkgo biloba flavonoids in an aqueous two-phase system (ATPS) formed from hydrophilic ionic liquid [C8 mim][N(CN)2](1-octyl-3-methylimidalium dicyanamide) and phase-forming salt (NH4)2SO4 was studied in this study.The effects of concentration of salt, temperature of system,pH value,amount of electrolyte of NaCl on extraction efficiency were investigated.The parameters influencing the recovery efficiency of (NH4)2SO4 were investigated.(NH4)2SO4 can be recovered from salt solution with higher recovery efficiency.When the ATPS contained 18.52% [C8 mim][N(CN)2]and 25.93% (NH4)2SO4,and 1.5 mmol NaCl was added at 298.15 K with pH being not adjusted,the extraction efficiency was 96.73%.IL/salt ATPS had higher extraction efficiency and lower viscosity than other ATPSs.When adding 2 volumes of methanol, (NH4)2SO4 can be recovered 90.54%.ATPS based on [C8 mim][N(CN)2]/(NH4)2SO4 was a good method on extraction and purification of Ginkgo biloba flavonoids.
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    Structural Characterization of a Heteropolysaccharide from Sargassum thunbergii and Its Anti-oxidant and Anti-colon Cancer Cells Proliferation Activity
    CAO Sen,ZHOU Rong,NIE Kai-ying,LUO Dian-hui*
    NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2017, 29 (10): 1635-1641.   DOI: 10.16333/j.1001-6880.2017.10.001
    Abstract1046)      PDF(pc) (839KB)(632)       Save
    DEAE-Sepharose CL-6B column chromatography was used to separate and purify a polysaccharide STP-I from the algae Sargassum thunbergii.The structural assignment of STP-I was performed using Fourier transform infrared spectroscopy (FTIR) analysis,periodate oxidation-smith degradation,methylation analysis and nuclear magnetic resonance (NMR).The repeating unit of STP-I was therefore determined.Major sugar residues of STP-I were6-β-D-Glcp and4-α-D-Galp, other residues including 5-α-L-Araf, 2,3-α-D-Xylp, T-β-D-Xylp and T-β-D-Glcp.The degree of branching (DB) of STP-I was 26.7%.On the basis of radical assays,STP-I exhibited higher scavenging activities of hydroxyl and superoxide radicals than Vitamin C with EC50 values of 0.15 and 1 mg/mL,respectively.STP-I also exhibited the capability of anti-proliferation in human colon cancer cells Caco-2 with IC50 value of 2.56 mg/mL,and cause little loss to common cells.
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    3D-QSAR Studies on Secolignansas as 5-alpha Reductase Inhibitors
    LI Xiao-qi,CAO Hong-yu,YU Da-yong*,FENG Bao-min*
    NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2016, 28 (5): 643-649.   DOI: 10.16333/j.1001-6880.2016.5.001
    Abstract1145)      PDF(pc) (901KB)(629)       Save
    5-Alpha reductase (5-AR) is a key enzyme in the treatment of benign prostatic hyperplasia.Previous studies found that the secolignansas had effect on the 5-AR as inhibitor.In order to study the nettle genus plant and explore the relationship between 5-AR and nonsteroidal inhibitor ecolignans.Homology modeling method was applied to build the three dimensional structure of 5-AR.The obtained protein structure was evaluated by Ramachandran Plot,verify3D and ERRAT program,and optimized by molecular mechanics methods.The optimal protein structure was used for the molecular docking test,the 5-alpha reductase inhibitors were ligands,docked into the active binding sites.The docking data and chart demonstrated that there were 12 binding sites,and 5 of them docked successfully.In addition,41 Secolignansas compounds properties were predicted by ADMET.These results provided the guidance to design new drugs,as well as new ideas for the treatment of benign prostatic hyperplasia.
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    A New Lactone Aldehyde Compound Isolated from Secondary Metabolites of Marine Fungus Penicillium griseofulvum
    XU Yan,TIAN Sha-sha,ZHU Hua-jie*
    NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2015, 27 (4): 559-561.   DOI: 10.16333/j.1001-6880.2015.04.001
    Abstract1285)      PDF(pc) (284KB)(628)       Save
    Three chemical constituents were separated and purified from marine-derived fungus Penicillium griseofulvum by silica gel column chromatography,semipreparative HPLC,ODS column chromatography and Sephadex LH-20 column chromatography.On the basis of spectral data (1D NMR,2D NMR and high resolution MS),their structures were elucidated as 2-(6-hydroxy-5,7-dimethylbenzofuranone-4-yl)acetaldehyde (1),terephthalic acid bis(2-ethyl-hexyl) ester (2) and bis(2ethylhexyl) adipate (3).Compound 1 was a new compound.
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    Chemical Constituents from Agrimonia pilosa Ledeb
    WU Hai-bo, LAN Xiao-cong, WANG Wen-shu
    NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2012, 23 (1): 55-56.   DOI: A
    Abstract2026)      PDF(pc) (228KB)(628)       Save
    The 95% ethanol extract of the whole medicinal plants Agrimonia pliosa Ledeb was separated by silica gel column chromatography and thin layer chromatography to afford four compounds, which were identified as stigmast-5-ene-3β,7β-diol (1), stigmast-5-ene-3β,7α-diol (2), stigmastane-3β,6α-diol (3) and β-sitosterol (4) on the basis of spectroscopic analysis and comparison with the reported data. Compounds 1-3 were isolated from A. pliosa for the first time.
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    Curcumin inducing apoptosis of U87 cells by promoting ROS production
    LIU Guo-an, JIN Ya-dong, RAN Miao-miao, LI Gui-chen, YANG Qi-li, DING Lan
    NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2020, 32 (4): 541-548.   DOI: 10.16333/j.1001-6880.2020.4.001
    Abstract580)      PDF(pc) (1714KB)(626)       Save

    Human glioma U87 cells were treated with curcumin and the effect on cell proliferation was assayed by CCK-8 method,the morphological changes were observed NADPH oxidase in cells were tested by ELISA.DCFH-DA fluorescence stain was used and the cells ROS content was detected.The oxidative stress indexes total antioxidant capacity(T-AOC),malondialdehyde(MDA),superoxide dismutase(SOD) and glutathione(GSH) were tested.Apoptosis was analyzed by AO/EB fluorescence staining and flowcytometry after double staining with Annexinv/PI.Finally,apoptosis-related signaling pathway protein was detected by immunoblotting.The results showed that curcumin promoted ROS production by increasing cell NADPH oxidase activity,which decreased intracellular T-AOC,increased MDA content,decreased GSH content,and increased SOD activity.These indicated curcmin induced oxidative stress in cells,and possibly increased ROS to trigger the cell signaling pathway by down-regulating the expression of NF-κB/p65 protein,and finally promoted apoptosis through the apoptosis-executing molecule Caspase-3.

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    Study on Integrated Extraction of Effective Components from Salvia miltiorrhiza Bunge and Preparation of Tanshinone II A Sodium Sulfonate
    NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2014, 26 (9): 1510-1516.  
    Abstract1308)      PDF(pc) (732KB)(625)       Save
    In this study,the synchronous integrated extraction of the effective components from Salvia miltiorrhiza was optimized.The optimum extraction condition was:80% as ethanol concentration,1:25 as ratio of solid to liquid,3 h as extraction time,60 ℃ as extraction temperature.The extract was then concentrated to the proportion of 1.05 followed by adding 4 times amount (mL/g) of water for sedimentation.The water-soluble fraction and sediment were then separated.The water-soluble fraction was applied to macroporous resin adsorption,elution,to afford two sub-fractions,containing Danshensu and protocatechuic aldehyde,respectively.The macroporous resin was further eluted with 40% ethanol to afford salvianolic acid B.The sediment was dried and dissolved in benzene.It was then filtrated and applied to neutral alumina column chromatography to afford each single component.The yields of the purified components using the optimized purification process were: protocatechuic aldehyde 3.6%,Danshensu 1.2%,salvianolic acid B 5.5%,tanshinone I 0.23%,tanshinone II A 3.8%,tanshinone II B 0.72%,cryptotanshinone 0.18%,salvia acid methyl ester 0.09%.Their respective purities were:98.3%,99.2%,91.7%,97.2%,98.8%,98.5%,97.6% and 98.3%.The preparation process of water soluble compound,tanshinone A sulfonate,was optimized by the steep slope experiment using mixture ratio,reaction time,reaction temperature,solution alkalinity as influencing factors.The yield and content were determined to be 86.2% and 96.7%,respectively.
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    Chemical Constituents from Ganoderma applanatum and Absolute Configuration Determination of New Compounds
    BU Qing-hua, #, LUO Qi#, CHENG Yong-xian
    NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2016, 28 (5): 651-654.  
    Abstract1067)      PDF(pc) (841KB)(617)       Save
    Eight compounds including two new compounds,namely shushenes A (1) and B (2),were isolated from the fruiting bodies of Ganoderma applanatum. Their structures were identified by means of spectroscopic methods.The structures of the known compounds are lucidulactone A (3),2,3-dihydroxy-1-(4-hydroxy3,5-dimethoxyphenyl)-1-propanone (4),6-hydroxy-4-H-chromen-4-one (5),1-(4-ethylphenyl)-1,2-ethanediol (6),1-(2-ethylphenyl)-1,2-ethanediol (7) and indole-3-carboxaldehyde (8),which were all isolated from this species for the first time.Of note,compounds 1 and 2 were isolated as racemic mixtures,chiral separation followed by computational methods allowed their absolute configuration assignment.In addition,inhibition of compounds 1 and 2 on fibronectin expression was tested by using an enzyme-linked immunosorbent assay (ELISA).
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    Apigenin Inducing Different Cells Apoptosis through Increasing ROS
    LIAO Yue-xia,KONG Gui-mei,TAO Wen-hua,BO Ping*
    NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2015, 27 (7): 1254-1258.   DOI: 10.16333/j.1001-6880.2015.07.024
    Abstract1254)      PDF(pc) (781KB)(610)       Save
    Apigenin is a naturally occurring plant flavonoid that possesses antioxidant,anti-cancer and anti-inflammatory properties.To investigate the effect of apigenin in mice spleen cells and mouse sarcoma S180 cells,the spleen cells and S180 cells were incubated with different concentrations of apigenin.The rates of viable cells were determined by MTT assay,apoptosis and the level of intercellular ROS were determined by flow cytometric analysis.The results showed that apigenin inhibited the proliferation of spleen cells and S180 cells,induced cell apoptosis and increased intercellular ROS.
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    Isolation, Anti-complement Activities, and Chemical Structures of Polysaccharide from Eucommia ulmoides Leaves
    ZHANG Xue-jun,YI Ting-jin,SUN Qian-yun,ZHANG Wen-kun,YAN Yin-ping,GONG Gui-zhen,GONG Ben-hong
    NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2011, 23 (4): 606-611.  
    Abstract3061)            Save
    After removal of resin and gutta percha under petroleum ether reflux, Eucommia ulmoides Leaves were decocted with distilled water and the extract was concentrated and fractionally precipitated by adding the one-fold,two-fold and three-fold volumes of alcohol to obtain three groups of crude polysaccharides, PsEUL1, PsEUL2, and PsEUL3. Of them, the group of polysaccharides, PsEUL1, was decolourized on a macro-porous resin (S-8) column and deproteinized using Sevag reagent.After dialysis,the refined PsEUL3 was applied to a diethylaminoethyl (DEAE)-cellulose anion exchange column and isolated into three monomeric polysaccharides, PsEUL11, PsEUL12, and PsEUL13. The assays of anti-complement activities indicated many fractions of polysaccharides have different extent of inhibitory activities with dose-effect relationships. PsEUL13 was demonstrated to be an acidic polysaccharide by reacting with sulfuric acid and carbazole, containing 21.2% of uronic acid. The gas chromatography revealed that it was composed of L-rhamnose, D-fucose, D-arabinose, D-xylose, D-glucose and D-galactose besides uronic acid, their percentage composition being 11.8, 1.6, 37.7, 4.2, 10.7 and 12.8, respectively.
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    Advances in Bacteriostatic Activity of Plant Essential Oil
    HU Lin-Feng, XU Ming-Lu, ZHU Hong-Xia
    NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2011, 23 (2): 384-391.  
    Abstract3583)      PDF       Save

    In this paper,advances on bacteriostatic activity and active ingredients of plant essential oil were summarized,which included 31 plant essential oils and 13 active ingredients. In addition,bacteriostatic mechanisms of essential oil and active ingredients were analysed.

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    A New Natural Product from Streptomyces sp.neau-D50
    ZHANG Hui, QI Huan, ZHANG Ling-jian, XIANG Wen-sheng, WANG Ji-dong
    NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2016, 28 (4): 495-497.  
    Abstract898)      PDF(pc) (2521KB)(601)       Save
    The chemical constituents of borrelidin-producing strain Streptomyces sp.neau-D50 was reinvestigated and a new natural product (N-acetylborrelidin B) was obtained. N-Acetylborrelidin B exhibited cytotoxic activity against human breast adenocarcinoma cell line MCF-7 and murine melanoma cell line B-16 with IC50 values of 19.9 μM and 36.3 μM,respectively.
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    Chemical Constituents from the Marine-derived Fungus Rhizopus sp. 2-PDA-61
    WANG Fa-Zuo, ZHU Tian-Jiao, ZHANG Min, LIN Ai-Qun, GU Qian-Qun
    NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2011, 23 (2): 199-201.  
    Abstract2833)      PDF       Save

    Two cytotoxic cyclicpentapeptides (1 and 2) and four benzene derivatives (3-6) have been isolated from the marine-derived fungus Rhizopus sp. 2-PDA-61. By spectral analysis and comparison the data with those of known compounds, they were identified as malformin C (1), malformin A1 (2), 2, 5-dihydroxyphenylacetic acid methyl ester (3), p-hydroxy phenylethanol (4), p-methyl phenylethanol (5), and p-dimethoxybenzene (6), respectively. Moreover, compounds 1 and 2 displayed moderate cytotoxicities to P388 cell lines, and the IC50 values of them were 1.8 μM and 4.2 μM.

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    Preparation,Characterization and Application of Ginsenoside Rg1bonded Silica Gel Stationary Phase(Ⅱ)
    GU Xiao-juan, HAN Ya-qiong, LI Xin-yi, SHEN Bao-chun*
    NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2014, 26 (6): 824-829.  
    Abstract1189)      PDF(pc) (681KB)(600)       Save
    A new ginsenoside Rg1-bonded silica gel stationary phase (250 mm×4.6 mm,5 μm) was prepared by one-pot reaction methodusing 1,6-diisocyanate as spacer arm.The characterization of the new self-made ginsenoside Rg1-bonded silica gel stationary was done by elemental analysis,thermal analysis and scanning electron microscope(SEM).The HPLC fingerprint of Active Pharmaceutical Ingredient (API) of Panax notoginseng saponins (PNS) on the ginsenoside Rg1-bonded silica gel stationary phase was established.The chromatographic fingerprints of 10 batches PNS API were determined on the self-made ginsenoside Rg1-bonded silica gel column.The mobile phase consisted of acetonitrile and water with gradient elution mode.The detection wavelength was 203 nm,the column oven temperature was maintained at 25 ℃,the flow rate was 0.8 mL/min and the injection volume was 10 μL.Based on the peak of ginsenoside Re,20 common peaks were selected,the similarity between 10 batches of samples were within 0.882-0.999.This study provided a new idea for natural products analysis using ginsenoside Rg1-bonded silica gel stationary phase.
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    Chemical Constituents and Their Toxic Activity from the Endophytic Fungus Phomopsissp. KY-12, Isolated from Pleioblastus amarus
    GUO Fang1, YANG Sheng-xiang1,2*, LIU Li1,2*, WANG Yong2
    NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2014, 26 (9): 1389-1392.  
    Abstract1405)      PDF(pc) (348KB)(595)       Save
    To study the chemical constituents and their toxic activity from the endophytic fungus Phomopsis sp. KY-12, isolated from Pleioblastus amarus. The compounds were isolated and purified by means of chromatographic techniques and their structures were identified on the basis of spectral features. Cytotoxic activity was performed by the brine shrimp (Artemiasalina) larvae bioassay.13known compounds including 9Cadinanesesquiterpenoids, 1 azaphilone and 3 steroids,named 3,12-dihydroxycalamenene (1), 3,9,12-trihydroxycalamenene (2), indicumolide C (3), agripilol C (4), 2,15-tihydroxycalamenene (5), dysodensiol D (6), bombamalones D (7), 8-formyl-7-hydroxyl-5-isopropyl-2-methoxy-1,4-naphthoquinone (8), strobilol A (9), Pyrenocine J (10), 3β, 5α-Dihydroxy-6β-methoxyergosta-7,22-dien (11), ergosterol (12) and ergosterol peroxide (13),were isolated from the organic extract of fermentation broths of the endophytic fungus, Phomopsis sp. KY-12. Compounds 3-9 were first isolated from the Phomopsis genus.Compounds 1-8 showed moderate active against the brine shrimp larvae,compound 9 indicated significant growth inhibitory activity against the brine shrimp (Artemias alina) larvae.
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    Optimization of Ultrasonic-assisted Extraction of Total Polyphenols from Agrimonia pilosa Ledeb.by Response Surface Methodology
    XU Xiu-quan,LI Feng,TANG Jian,XU Yuan
    NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2012, 23 (1): 114-117.   DOI: A
    Abstract2096)      PDF(pc) (732KB)(595)       Save
    Response surface methodology (RSM) was used to optimize ultrasonic-assisted extraction for total polyphenols from Agrimonia pilosa Ledeb.based on single-factor experiment.The main factors extraction temperature,liquid-material ratio and ethanol concentration were determined.The relationship between influencing factors and response values was established.The optimum extraction conditions were as follows: the extracting solvent was 71% ethanol,solid-liquid ratio was 1:24,extracting temperature was 60℃,and extracting time was 20 min.Under these conditions,the maximal yield of total polyphenols reached 3.56%,which was well matched with the predicted concent.
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    Secondary Metabolites from Deep-Sea-Derived Fungus Penicillium herquei FS83
    LI Hao-hua, PAN Qin-ling, CHEN Yu-chan, LI Sai-ni, TAN Guo-hui, YE Wei, LIU Hong-xin, SUN Zhang-hua, ZHANG Wei-min
    NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2016, 28 (3): 328-330.  
    Abstract1036)      PDF(pc) (311KB)(592)       Save
    Five compounds were isolated from the extract of the deep-sea-derived fungus Penicillium herquei FS83 by utilizing various chromatographic methods such as silica gel,reverse silica gel,Sephadex LH-20,preparative TLC and HPLC.Their structures were identified as peniherin A (1),sclerodin (2),methyl (4-OH) phenylacetate (3),4-hydroxyacetophenone (4),(R)-mevalonolactone (5),respectively,by spectroscopic data.Compound 1 was identified as a new compound,and compound 2 was isolated from Penicillium for the first time.No cytotoxic and antibacterial activities were detected for compound 1.
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    Optimization of Ultrasonic-assisted Extraction of Phospholipid Fatty Acids from Forest Soil by Response Surface Analysis
    LI Fan1, LI Na2*, CHEN Jian-zhong2, JIANG Xiao-qiang2, CHEN Yi2, LI Ping1
    NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2014, 26 (5): 656-661.  
    Abstract1286)      PDF(pc) (842KB)(590)       Save
    The objective of this study was to optimize ultrasonic-assisted extraction of phospholipid fatty acids (PLFAs) from forest soil using response surface methodology (RSM). Based on single factor experiments,extraction temperature,time and amount of extraction solvent were selected for Box-Behnken central composite designing. RSM was employed to study the effect of these factors on the yield of PLFAs.The results indicated that the optimal extraction conditions were 20 ℃ as extraction temperature,21 mL as amount of extraction solvent,with 30 min of extraction.Under these conditions,the maximal extraction yield reached 45.57 nmol/g (n=3).The response surface methodology used for the optimization of ultrasonic-assisted extraction conditions of PLFAs from forest soil was simple and reliable.
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    Flavonoids from the Twigs of the Tibetian Medicine Myricaria germanica
    LA Xiao-qin,ZENG Yang,XU Min,ZHANG Ying-jun
    NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2011, 23 (4): 596-599.  
    Abstract3528)            Save
    Eleven flavonoids were isolated from the 60% aq.acetone extract of the air-dried twigs of Myricaria germanica (Tamaricaceae),a famous Tibetian medicine.Their structures were elucidated by spectroscopic analysis and identified as 3,5,4'-trihydroxy-7,3'-dimethoxyflavone (1),3,5,4'-trihydroxy-7-methoxyflavone (2),rhamnetin (3),3,5,7-trihydroxy-4'-methoxyflavone (4),tamarixetin (5),kaempferol (6),quercetin-3-O-β-D-glucoside (7),kaempferol-3-O-β-D-glucuronic acid (8),quercetin-3-O-β-D-glucuronicacid (9),quercetrin (10),and afzelin (11),respectively.All compounds were obtained from M.germanica for the first time,and compounds 4,5,711 were obtained from the genus Myricaria for the first time.
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    Secondary Metabolites from Deep-Sea-Derived Fungus Penicillium herquei FS83
    LI Hao-hua,PAN Qin-ling,CHEN Yu-chan,LI Sai-ni,TAN Guo-hui,YE Wei,LIU Hong-xin,SUN Zhang-hua*,ZHANG Wei-min*
    NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2016, 28 (3): 327-330.   DOI: 10.16333/j.1001-6880.2016.3.001
    Abstract1107)      PDF(pc) (392KB)(581)       Save
    Five compounds were isolated from the extract of the deep-sea-derived fungus Penicillium herquei FS83 by utilizing various chromatographic methods such as silica gel,reverse silica gel,Sephadex LH-20,preparative TLC and HPLC.Their structures were identified as peniherin A (1),sclerodin (2),methyl (4-OH) phenylacetate (3),4-hydroxyacetophenone (4),(R)-mevalonolactone (5),respectively,by spectroscopic data.Compound 1 was identified as a new compound,and compound 2 was isolated from Penicillium for the first time.No cytotoxic and antibacterial activities were detected for compound 1.
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    Prediction of Critical Flux and Pressure of Enzymolsis Extraction Ultrafiltration Purification for Fibrous Rhizome Herbs Based on BP Neural Network
    WANG Ji-long,LIU Xiao-xia,WEI Shu-chang*,LIU Chun,FAN Ling-yun,JIN Hui
    NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2016, 28 (4): 586-590.   DOI: 10.16333/j.1001-6880.2016.4.021
    Abstract889)      PDF(pc) (677KB)(580)       Save
    The aim of this study was to effectively solve the problem of membrane fouling when integrated techniques of the enzymolsis extraction-ultrafiltration purification were used in fibrous rhizome herbs and keep the integrated techniques using smoothly.The prediction model of critical flux and pressure was established based on the ultrafiltration data of enzymatic hydrolysate of Hedysari Radix by BP neural network.The performance and applicability of the model were evaluated.Then sensitivity analysis of input variables were performed using connection weights method to assess the relative contribution of input variables.Results indicated that the model had better performance and applicability.Mean absolute error,mean error rate and R2 of critical flux and pressure were 1.5228 L/(m2·h) and 0.0032 MPa,3.46% and 2.50%,0.96 and 0.95,respectively.Mean absolute error and mean error rate of critical flux and pressure for Astragali Radix were 1.4360 L/(m2·h) and 0.0034 MPa,3.93% and 2.80%,respectively.The relative contribution of input variables to critical flux and pressure presented the same order of viscosity>concentration>pH>temperature.
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    Chemical Constituents of Enzymatic Hydrolysate of Total Ginsenoside Extract of Panax quinquefolium
    JIN Jian-ming 1,2*, LI You-hai2, ZHANG Hai-lun1, LIANG Chao-ning3, TANG Shuang-yan3
    NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2014, 26 (10): 1552-1556.  
    Abstract1429)      PDF(pc) (414KB)(574)       Save
    The total ginsenoside extract of Panax quinquefolium  was hydrolyzed by β-glycosidase.HPLC analysis indicated that ginsenosides Rb1,Rd,Rc and Rb2 were completely hydrolyzed.Seven ginsenosides were isolated from the hydrolysate,and identified as ginsenoside compound K (1),ginsenoside Mc (2),ginsenoside Rg1 (3),ginsenoside Rg2 (4),ginsenoside Re (5),ginsenoside F1 (6) and pseudoginsenoside F11 (7),respectively.The protopanaxdiol type ginsenosides in the total ginsenoside extract of P.quinquefolium were converted into ginsenoside compound K and ginsenoside Mc by the β-glycosidase.
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    A New Method for the Preparation of High Purity Egg Yolk Lecithin Used for Injection
    ZHAO Hui-hui, TANG Lu-hong*
    NATURAL PRODUCT RESEARCH AND DEVELOPMENT    2014, 26 (4): 564-569.  
    Abstract1255)      PDF(pc) (623KB)(564)       Save
    A new process was explored and established for the preparation of high purity egg yolk lecithin used for injection using fresh egg yolk as raw material,ethanol as the only extraction solvent.First of all,4 parts 96% (v/v) ethanol was used to precipitate the protein from the fresh egg yolk.The egg yolk protein as filter cake and the primary extract as filtrate were collected separately;after that,the remaining lecithin in filter cake was extracted by 4 parts 96% (v/v) ethanol for 3 times as the secondary extract.Then the primary and secondary extract were combined and stored at 9 ℃ over night to remove most of the egg yolk oil.Finally,the resulting supernatant was separated via silica gel column with 86% (v/v) ethanol as eluent,and the purity of egg yolk lecithin fraction,detected by HPLC,reached to 98%.Meanwhile,the extraction process was optimized as follows:for precipitation,35 ℃,the ratio of 96% (v/v) ethanol to fresh egg yolk was 4:1 (w/w),with 180 rpm stirring speed for 10 min;for extraction,60 ℃,the ratio of 96% (v/v) ethanol to filter cake was 4:1,standing 15 min for 3 times.Under optimized conditions,the extraction yield of egg yolk lecithin reached to 93.38%.
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