Abstract: To explore the effects of drugs containing SD rats serum in the different body parts of Ningxia dense point lizard on the apoptosis of the line SGC-7901 human gastric cancer cell,and its anticancer mechanism.The SPF male SD rats were randomly divided into different groups,given corresponding drug intervention to prepare drug containing serum,added to gastric cancer cells,and detected cell viability by MTT,the cell apoptosis with Annexin V-FITC/PI double staining,and the expression of Sirt1 and P53 protein in gastric cancer cells by Western-blot.The results showed that the expression levels of Sirt1 and P53 proteins could be reduced significantly in different parts of the lizard,inhibiting cell proliferation and promoting apoptosis.In summary,different parts of Ningxia dense point lizard may inhibit Sirt1,reduce P53,and induce apoptosis,and the anti-tumor effect of Ningxia dense point lizard tail is the most significant.

Key words: Ningxia dense point lizard, containing drug Sserum, human gastric cancer cells, cells apoptosis