天然产物研究与开发 ›› 2016, Vol. 28 ›› Issue (1): 107-111.doi: 10.16333/j.1001-6880.2016.1.019

• 开发研究 • 上一篇    下一篇

河西走廊5种沙棘果主要活性成分及对ROS清除作用比较分析

范惠玲*,白生文,张芬琴,王进   

  1. 河西学院农业与生物技术学院,张掖 734000
  • 出版日期:2016-01-29 发布日期:2016-02-01

Main Active Components and ROS Scavenging Effects of Five Hippophae rhamnoides L. Cultivars from Hexi Corridor

FAN Hui-ling*,BAI Sheng-wen,ZHANG Fen-qin,WANG Jin   

  1. College of Agriculture and Biotechnology,Hexi University,Zhangye 734099,China
  • Online:2016-01-29 Published:2016-02-01

摘要: 为了探明河西走廊沙棘果中的主要化学成分及对活性氧自由基的清除效应,本试验以5个沙棘品种,包括中国沙棘、西藏沙棘、肋果沙棘、中亚沙棘和蒙古沙棘为材料,对其鲜果中所含的总酚类、黄酮类和维生素C的含量分别进行了测定,并就沙棘果甲醇提取液对3种活性氧的抑制率进行了分析。结果表明:(1)5个沙棘品种中,肋果沙棘果中总黄酮含量最高,达5.25 g芦丁/kg鲜重,西藏沙棘果次之;总酚类含量介于8.25~13.26 g没食子酸/kg鲜重之间,Vc含量介于1.84~3.46 g/kg鲜重之间,其中肋果沙棘和西藏沙棘的总酚类和Vc含量均较高。(2)肋果沙棘和西藏沙棘果甲醇提取液对NO·、O2和·OH的清除作用均较高。(3)总黄酮类与3种ROS的清除率间的相关性最大。总之,肋果沙棘和西藏沙棘果不仅具有高含量的活性物质,其甲醇提取液对氧自由基的清除活性也较强。

关键词: 沙棘, 黄酮类, 多酚类, 维生素C, 自由基清除

Abstract: The aim of this work was to determine the total contents of phenols,flavonoids and Vc in the fruits of 5 selected Hippophae rhamnoides L. cultivars from Hexi corridor and to evaluate the scavenging activity of H. rhamnoides fruits methanol extracts on reactive oxygen species (ROS),namely hydroxyl radical (·OH),nitric oxide (NO·),superoxide anion (O2). Altogether,5 cultivars of this fruit species presented total phenolic contents ranging from 8.25-13.26 g of gallic acid/kg fresh weight,Vc contents ranging from 1.843.46 g/kg fresh weight,flavonoids contents ranging from 3.68-5.25 g rutin/kg fresh weight,and high contents of phenols,flavonoids and Vc were obtained from H. neurocarpa S.W. and H. thibetana Schlecht fruits. The crude methanol extracts from H. neurocarpa and H. thibetana fruits showed high inhibitory effects on reactive ROS. There were high correlations between the flavonoids contents and the ROS scavenging effects. The obtained results would contribute to the popularization of H. rhamnoides as a promising source for the food industry.

Key words: Hippophae rhamnoides L., flavonoids, phenolics, Vc, radical scavenging

中图分类号: 

R151.2