关键词: 兰科, 美花石斛, DPPH自由基清除, 酪氨酸酶抑制活性, 胶原蛋白分泌

Abstract: To study the chemical constituents and explore the skin caring activity of Dendrobium loddigesii,all compounds were isolated by chromatography with silica gel,Sephadex LH-20,MCI gel and Lichroprep RP-18 gel and identified by NMR and MS;Twelve compounds were isolated from D.loddigesii,which were moscatin (1),2,4,7-trihydroxyl-9.10-dihydrophenanthrene (2),3,6,9-trihydroxy-3,4-dihydroanthracen-1(2H)-one (3),3,5′-dimethoxy-3′,4,9′-trihydroxy-7′,9-epoxy-8,8′-lignan (4),trigonopol B (5),crepidatuol B (6),syringaresinol (7),isovanillin (8),coniferyl aldehyde (9),2-methoxyphenol-5-hydroxymethyl (10),dihydroconiferyl alcohol (11),4-hydroxy-3- methoxyphenylethanol (12).And compound 3-5,7-12 were isolated from this plant for the first time.Compound 1,2,6 could scavenge almost 80% DPPH radical at 100 μg/mL,compound 2 could promote 25.6% secretion of collagen at 10 μg/mL.
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Key words: Orchidaceae, Dendrobium loddigesii, DPPH radical scavenger, tyrosine kinase inhibitory, collagen secretion