UPLC-QTOF/MSE联合UNIFI筛选平台快速分析牛膝中三萜皂苷类成分

doi:10.16333/j.1001-6880.2019.6.020

天然产物研究与开发 ›› 2019, Vol. 31 ›› Issue (6): 1054-1061.doi: 10.16333/j.1001-6880.2019.6.020

UPLC-QTOF/MS^E联合UNIFI筛选平台快速分析牛膝中三萜皂苷类成分

傅俊1,2，吴欢1,2，吴虹1,2*

1安徽中医药大学药学院，合肥 230012；2新安医学教育部重点实验室，合肥 230038

出版日期:2019-06-26 发布日期:2019-06-26
基金资助:
国家自然科学基金面上项目（81473400）；安徽中医药大学校级探索性科研项目（2017HXTS37）

Rapid characterization of triterpenoid saponins in Achyranthes bidentate Blume by UPLC-Q-TOF/MS in MS^E mode combined with UNIFI screening platform

FU Jun1,2,WU Huan1,2,WU Hong1,2

1Anhui University of Chinese Medicine,Hefei 230012,China; 2Key Laboratory of Xin'an Medicine,Ministry of Education,Hefei 230038,China

Online:2019-06-26 Published:2019-06-26

摘要/Abstract

摘要： 为明确牛膝三萜皂苷主要化学成分，采用超高效液相色谱-四极杆飞行时间质谱（UPLC-QTOF/MS）联合UNIFI科学信息系统对牛膝中的三萜皂苷进行快速定性分析。首先，经UPLC色谱分离后的牛膝三萜皂苷化学成分群在MSE模式下采集质谱数据；然后，通过UNIFI平台自动检出并经过MassLynx工作站进行逐个核对，以快速完成牛膝三萜皂苷化学成分群的定性分析。本实验共分离、鉴定了40种三萜类皂苷类成分，其中以齐墩果酸为母核的化合物有38种，另有其它母核的三萜皂苷化合物2种。该方法可以快速的对牛膝中的三萜皂苷进行全面分析，将为牛膝皂苷的质量控制及后续药效物质研究提供参考。

关键词: UPLC-QTOF/MS^E, 牛膝, UNIFI, 三萜皂苷

Abstract: To investigate the main chemical constituents of Achyranthes bidentate triterpenoid saponins,a rapid qualitative analysis of triterpenoid saponins in Achyranthes bidentata was carried out by ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTOF/MS) in MSE mode combined with UNIFI scientific information system.Firstly,the chemical constituents of Achyranthes bidentata triterpenoid saponins were separated by UPLC chromatography and the mass spectrometry data were collected by QTOF/MS in MSE mode.Then,the chemical constituents of Achyranthes bidentata triterpenoid saponins were screened automatically through UNIFI platform and checked by MassLynx workstation,so as to quickly complete the qualitative analysis of the chemical constituents of Achyranthes bidentata triterpenoid saponins.AS a result,forty triterpenoid saponins were isolated and identified in this experiment.Among them,there are 38 kinds of compounds with oleanolic acid as the mother nucleus,and two triterpenoid saponins with other mother nucleus.This method can quickly analyze the triterpenoid saponins in Achyranthes bidentata,which will provide reference for the quality control of Achyranthes bidentata saponins and the study of subsequent pharmacodynamic substances.

Key words: UPLC-QTOF/MS^E, Achyranthes bidentate, UNIFI, triterpenoid saponins

中图分类号:

R917 R284.2

傅俊1,2，吴欢1,2，吴虹1,2*. UPLC-QTOF/MS^E联合UNIFI筛选平台快速分析牛膝中三萜皂苷类成分[J]. 天然产物研究与开发, 2019, 31(6): 1054-1061.

FU Jun1,2,WU Huan1,2,WU Hong1,2. Rapid characterization of triterpenoid saponins in Achyranthes bidentate Blume by UPLC-Q-TOF/MS in MS^E mode combined with UNIFI screening platform[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2019, 31(6): 1054-1061.

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UPLC-QTOF/MS^E联合UNIFI筛选平台快速分析牛膝中三萜皂苷类成分

Rapid characterization of triterpenoid saponins in Achyranthes bidentate Blume by UPLC-Q-TOF/MS in MS^E mode combined with UNIFI screening platform

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UPLC-QTOF/MSE联合UNIFI筛选平台快速分析牛膝中三萜皂苷类成分

Rapid characterization of triterpenoid saponins in Achyranthes bidentate Blume by UPLC-Q-TOF/MS in MSE mode combined with UNIFI screening platform

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UPLC-QTOF/MS^E联合UNIFI筛选平台快速分析牛膝中三萜皂苷类成分

Rapid characterization of triterpenoid saponins in Achyranthes bidentate Blume by UPLC-Q-TOF/MS in MS^E mode combined with UNIFI screening platform