To study the mechanism of Danshen-Chuanxiong(DS-CX) in the treatment of Alzheimer's disease(AD) by using network pharmacology.Through database search and literature retrieval,the effective ingredients of DS and CX were obtained,and the targets of active ingredients were obtained through STITCH and TCMSP databases.And the drugs-targets network and PPI network were built.Then,gene ontology (GO) analysis and KEGG metabolic pathway enrichment analysis were performed on the action targets of DS-CX were performed using Metascape database.Finally,MTT,ELISA,flow cytometer and fluorescence quantitative PCR experiment were carried out to verified the protective effect of DS-CX on PC12 cells.The results showd that 25 active components of DS-CX were screened and 105 targets for the treatment of Alzheimer's disease,top 20 target molecular functions by GO analysis,and top 10 important signaling pathways.Cell experiments also proved that DS-CX significantly increased the cell survival rate of PC12 cells and antagonize the inflammatory response of PC12 cells and reduce the apoptosis rate of PC12 cells,and inhibited the activation of p38MAPK/NF-κB signaling pathway.DS-CX couplet medicines had a variety of active ingredients that interact with multiple targets and multiple pathways in a synergistic manner to play a major therapeutic role on AD by anti-apoptosis and anti-inflammatory,which provides the theoretical basis for its clinical application.