南方红豆杉菌根紫杉醇含量及其菌根菌组分离纯化和种属鉴定

doi:10.16333/j.1001-6880.2022.5.008

天然产物研究与开发 ›› 2022, Vol. 34 ›› Issue (5): 800-809.doi: 10.16333/j.1001-6880.2022.5.008

南方红豆杉菌根紫杉醇含量及其菌根菌组分离纯化和种属鉴定

彭凤珍1，杜亚填1*，周春长2，龚雪元3

1吉首大学林产化工工程湖南省重点实验室，张家界 427000；2浙江大学基础医学院，杭州 310000；3基蛋生物科技股份有限公司，南京 210044

出版日期:2022-05-28 发布日期:2022-05-23
基金资助:
国家自然科学基金地区基金（31660077）

Paclitaxel content in mycorrhizal of Taxus chinensis var. mairei and isolation purification and species identification of its fungal

PENG Feng-zhen1,DU Ya-tian1 *,ZHOU Chun-chang2,GONG Xue-yuan3

1Key Laboratory of Hunan Forest Products and Chemical Industry Engineering,Jishou University,Zhangjiajie 427000,China;2School of Basic Medicine Sciences Zhejiang University,Hangzhou 310000,China;3Getein Biotech Incorporated Company,Nanjing 210044,China

Online:2022-05-28 Published:2022-05-23

摘要/Abstract

摘要：

本文采用高效液相色谱、组织培养、形态学观测结合真菌核糖体转录间隔区（rDNA-ITS）序列生物学分析等方法，进行了南方红豆杉（Taxus chinensis var. mairei）菌根组织解剖学各部位、茎段愈伤组织及其与菌根真菌互作后紫杉醇含量、菌根菌组分离纯化和种属鉴定分析等研究。结果显示，菌根原始根组织中紫杉醇的含量比须根高0.645 mg/g，比其他器官组织高1个数量级，且南方红豆杉茎段愈伤组织与菌根真菌互作可提高愈伤组织紫杉醇的含量。菌根菌组经分离纯化共获得26个菌株，分属3门6纲9目11科14属，分别为曲霉属（Aspergillus）、木霉菌属（Trichoderma）、镰孢霉属（Fusarium）、青霉属（Penicillium）、Cylindrodendrum、小帚梗柱孢属（Cylindrocladiella）、螺旋聚孢霉属（Clonostachys）、炭角菌属（Xylaria）、枝孢霉属（Cladosporium）、棒孢属（Corynespora）、Paraboeremia、无柄盘菌属（Pezicula）、刺盘孢属（Colletotrichum）、烟管菌属（Bjerkandera），其中前两属为优势菌属，表明南方红豆杉菌根真菌存在种属多样性，且发现与其根、树皮、韧皮、木材、叶等器官组织中的内生真菌菌群的种类多样性间存在较大差异，说明菌根真菌与南方红豆杉未木栓化根共生互作是其具有较高紫杉醇积累的生物学基础。本文为南方红豆杉菌根较高紫杉醇含量与菌根真菌间的相关性研究奠定了基础。

关键词: 南方红豆杉, 植物内生菌, 菌根, 菌根真菌, 菌种分离, 种属鉴定

Abstract:

In this article,HPLC,tissue culture,morphological observation and biological analysis of fungal ribosomal transcription spacer (rDNA-ITS) sequence were used to research on the paclitaxel content in the mycorrhizal tissue aratomy each parts,stem callus of Taxus chinensis var. mairei and the stem callus after interaction with mycorrhizal fungi,and the isolation,purification and species identification of mycorrhizal fungi group.The results showed that the paclitaxel content in the mycorrhizal primordial root tissue of T. chinensis var. mairei was 0.645 mg/g higher than that of fibrous root,which was one order of magnitude higher than that of other organ tissues,and the interaction between stem callus and mycorrhizal fungi of T. chinensis var. mairei could increase the content of paclitaxel.26 strains of mycorrhizal fungi that were isolated and purified from the mycorrhizal,belonging to three phyla,six classes,nine orders, 11 families and 14 genera, including Aspergillus, Trichoderma, Fusarium, Penicillium, Cylindrodendrum, Cylindrocladiella, Clonostachys,Xylaria,Cladosporium,Corynespora,Paraboeremia,Pezicula, Colletotrichum and Bjerkandera.The first two genera are the dominant genera.The results showed that there were species diversity of the mycorrhizal fungi in the mycorrhizal of T. chinensis var. mairei ,and it was found that there were great differences between the species diversity of the mycorrhizal fungi in the mycorrhizal of T. chinensis var. mairei and the species diversity of the endophytic fungi in the other organs and tissues as its roots,bark,phloem,wood and leaves,and indicating that the symbiotic interaction between mycorrhizal fungi and non corked roots of T. chinensis var. mairei is the biological basis for its high paclitaxel accumulation.This paper provides a basis for the research on the correlation between its higher paclitaxel content and mycorrhizal fungi.

Key words: Taxus chinensis , var. , mairei , , plant endophytes, mycorrhiza, mycorrhizal fungus, strain isolation, species identification

中图分类号: Q36

彭凤珍, 杜亚填, 周春长, 龚雪元. 南方红豆杉菌根紫杉醇含量及其菌根菌组分离纯化和种属鉴定[J]. 天然产物研究与开发, 2022, 34(5): 800-809.

PENG Feng-zhen, DU Ya-tian , ZHOU Chun-chang, GONG Xue-yuan. Paclitaxel content in mycorrhizal of Taxus chinensis var. mairei and isolation purification and species identification of its fungal [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2022, 34(5): 800-809.

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南方红豆杉菌根紫杉醇含量及其菌根菌组分离纯化和种属鉴定

Paclitaxel content in mycorrhizal of Taxus chinensis var. mairei and isolation purification and species identification of its fungal

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