天然产物研究与开发 ›› 2022, Vol. 34 ›› Issue (5): 842-847.doi: 10.16333/j.1001-6880.2022.5.013

• 开发研究 • 上一篇    下一篇

人参皂苷Rb1对MPTP诱导的神经元损伤和小鼠行为学异常的作用研究

耿艺娟1,2，孙梦桢1,2，赵进东1,2，宋京风1*，胡炜彦1,2*   

  1. 1昆明医科大学 药学院暨云南省天然药物药理重点实验室；2昆明医科大学 生物医学工程中心，昆明 650500
  • 出版日期:2022-05-28 发布日期:2022-05-23
  • 基金资助:
    国家自然科学基金（81860254）;云南省自然科学基金（202101AY070001-009，2019FE001-031）;云南省专家工作站（202005AF150043）

Study on the effect of ginsenoside Rb1 on MPTP-induced neuronal injury and behavioral impairment in mice

GENG Yi-juan1,2,SUN Meng-zhen1,2,ZHAO Jin-dong1,2,SONG Jing-feng1*,HU Wei-yan1,2*   

  1. 1School of Pharmaceutical Science and Yunnan Key Laboratory of Natural Drug Pharmacology,Kunming Medical University; 2Biomedical Engineering Center,Kunming Medical University,Kunming 650500,China
  • Online:2022-05-28 Published:2022-05-23

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摘要：

探讨人参皂苷Rb1对1-甲基-4-苯基-1,2,3,6-四氢吡啶（1-methyl-4-phenyl-1,2,3,6-tetmhydropyridine，MPTP）诱导的小鼠多巴胺能神经元损伤及小鼠行为学异常的作用及可能的机制。体外分离培养小鼠多巴胺能神经元，给予MPTP（10 μmol/L）损伤和不同剂量的Rb1（10、20、40 μmol/L）药物治疗处理，观察神经元形态。对C57B/L6小鼠腹腔注射MPTP（30 mg/kg）构建帕金森模型，灌胃给予不同剂量Rb1（10、20、40 mg/kg）药物治疗，采用旷场实验及爬杆实验对小鼠进行行为学检测；采用蛋白免疫印迹反应（Western blot）检测纹状体TH、CaMKII、FP1和Bcl-2的表达。结果显示，与对照组相比，不同剂量人参皂苷Rb1可以明显改善MPTP诱导的神经元损伤；人参皂苷Rb1处理能显著改善MPTP诱导的PD模型小鼠行为学异常；人参皂苷Rb1可以显著上调MPTP所造成的TH、Bcl-2的减少，对CaMKII及FP1表达的减少没有显著作用。人参皂苷Rb1对MPTP诱导的神经元损伤和小鼠行为学异常具有明显的改善作用，其机制可能与逆转TH和Bcl-2的表达有关。

关键词: 人参皂苷Rb1, MPTP, 行为学, Bcl-2, TH

Abstract:

To investigate the effects and possible mechanisms of ginsenoside Rb1 on 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP)-induced dopaminergic neuronal injury and behavioral abnormalities in mice.In vitro isolated and cultured mouse dopaminergic neurons were treated with MPTP (10 μmol/L) and different doses of Rb1 (10,20,40 μmol/L),and neuronal morphology was observed.A Parkinson's model was constructed by intraperitoneal injection of MPTP (30 mg/kg) into C57B/L6 mice,and different doses of Rb1 (10,20,40 mg/kg) were given by gavage,and mice were subjected to behavioral tests using open field test and rod climbing test;Western blot was used to detect striatal TH,CaMKII,FP1 and Bcl-2 expression.The results showed that different doses of ginsenoside Rb1 significantly improved MPTP-induced neuronal injury compared with control group.Ginsenoside Rb1 treatment significantly improved the behavioral abnormalities in MPTP-induced PD model mice.Ginsenoside Rb1 significantly upregulated the reduction of TH and Bcl-2 caused by MPTP,and had no significant effect on the reduction of CaMKII and FP1 expression.Ginsenoside Rb1 had significant ameliorative effects on MPTP-induced neuronal injury and behavioral abnormalities in mice,and the mechanism may be related to the reversal of TH and Bcl-2 expression.

Key words: ginsenoside Rb1, MPTP, behavioral, Bcl-2, TH

中图分类号:  R285.5

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