麻欠精油对脂多糖诱导的THP-1细胞氧化应激的影响

doi:10.16333/j.1001-6880.2016.6.004

天然产物研究与开发 ›› 2016, Vol. 28 ›› Issue (6): 833-838.doi: 10.16333/j.1001-6880.2016.6.004

麻欠精油对脂多糖诱导的THP-1细胞氧化应激的影响

甘小青1,2，郭娟1，张红霞3，杜芝芝3，张萍1*

1 中国科学院西双版纳热带植物园热带植物资源可持续利用重点实验室，西双版纳 666303；2 中国科学院研究生院，北京 100049；3 中国科学院昆明植物研究所，昆明 650201

出版日期:2016-06-30 发布日期:2017-01-17

Effect of Maqian (Zanthoxylum myriacanthum var. pubescens Huang) Essential Oil on Oxidative Stress in THP-1 Cells Stimulated by LPS

GAN Xiao-qing1,2,GUO Juan1,ZHANG Hong-xia3,DU Zhi-zhi3,ZHANG Ping1*

1 Key Laboratory of Tropical Plant Resources and sustainable Use,Xishuangbanna Tropical Botanical Garden,Chinese Academy of Sciences,Xishuangbanna 666303,China;2 Univercity of Chinese Academy of Science,Beijing 10049,China;3Kunming Institute of Botany,Chinese Academy of Sciences,Kunming 650201,China

Online:2016-06-30 Published:2017-01-17

摘要/Abstract

摘要： 本研究主要探讨麻欠精油对脂多糖（LPS）诱导的THP-1细胞氧化应激的影响。溶剂对照、地塞米松或不同浓度的麻欠精油预处理THP-1细胞后，再用LPS培养24 h，用DCFH-DA荧光探针标记细胞后流式检测细胞内活性氧ROS；用FITC Annexin V/PI双染法流式检测细胞凋亡；用Griess试剂测定NO；用比色法检测总超氧化物歧化酶（SOD）；用Western Blot法检测NADPH p47 phox的表达。结果发现麻欠精油处理组与溶剂对照组相比，LPS诱导的ROS和NO水平显著降低、SOD水平显著增加、NADPH p47 phox蛋白表达减低，细胞凋亡降低。以上实验结果表明麻欠精油对LPS诱导的THP-1细胞的氧化应激有明显抑制作用并对细胞凋亡有保护作用。

关键词: 麻欠精油, THP-1, 氧化应激, 细胞凋亡

Abstract: In this study,the effect of Maqian (Zanthoxylum myriacanthum var. pubescens Huang) essential oil (MQEO) on oxidative stress in lipopolysaccharide (LPS)stimulated THP-1 cells was investigated.Cells were pretreated with vehicle,Dex or different doses of MQEO,and then cultured with LPS for 24 h.The intracellular reactive oxygen species (ROS) were determined by staining the cells with 2′,7′-ichlorofluorescein diacetate (DCFH-DA) followed by flow cytometry analysis.Cells were stained with Annexin V-FITC/PI and assayed for apoptosis by flow cytometry.NO was determined by Griess reagent method.Cell lysates were prepared and assayed for the activity of intracellular superoxide dismutase (SOD) by a colorimetric method and the expression of p47 phox (the cytoplasmic subunit of NADPH oxidase) by western blotting.The results showed that,compared with cells treated with vehicles,cells treated with MQEO exhibited significantly reduced LPS stimulated ROS and NO production in a concentration-dependent manner.In addition,cells treated with MQEO showed significantly less LPS-induced decrease in SOD activity as well as the increase of expression of cytoplasmic subunit of NADPH oxidase (p47 phox). Furthermore,cells treated MQEO exhibited markedly reduced LPS-stimulated apoptosis.Collectively,these findings suggested that MQEO had the ability to suppress oxidative stress in LPS-stimulated THP-1 cells and protect cells from apoptosis.MQEO had the potential application for the prevention and treatment of various diseases related to oxidative stress.

Key words: Maqian essential oil, THP-1, oxidative stress, apoptosis

中图分类号:

R967

甘小青1,2，郭娟1，张红霞3，杜芝芝3，张萍1*. 麻欠精油对脂多糖诱导的THP-1细胞氧化应激的影响[J]. 天然产物研究与开发, 2016, 28(6): 833-838.

GAN Xiao-qing1,2,GUO Juan1,ZHANG Hong-xia3,DU Zhi-zhi3,ZHANG Ping1*. Effect of Maqian (Zanthoxylum myriacanthum var. pubescens Huang) Essential Oil on Oxidative Stress in THP-1 Cells Stimulated by LPS[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2016, 28(6): 833-838.

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麻欠精油对脂多糖诱导的THP-1细胞氧化应激的影响

Effect of Maqian (Zanthoxylum myriacanthum var. pubescens Huang) Essential Oil on Oxidative Stress in THP-1 Cells Stimulated by LPS

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