天然产物研究与开发 ›› 2018, Vol. 30 ›› Issue (3): 425-428.doi: 10.16333/j.1001-6880.2018.3.013

• 研究简报 • 上一篇    下一篇

白芷美白活性部位初步研究

李海馨#,何婧芝#,冯文亚,赵倩,赵卓,陈佳露,吴卫,侯凯*   

  1. 四川农业大学农学院,成都 611130
  • 出版日期:2018-04-08 发布日期:2018-04-08
  • 基金资助:

    大学生创新训练计划(2015038);四川省突破性道地药材育种材料与方法创新(2016NYZ0036)

Preliminary Studies on the Active Fraction Responsible for Whitening Activity of Angelicae Dahuricae

LI Hai-xin#,HE Jing-zhi#,FENG Wen-ya,ZHAO Qian,ZHAO Zhuo,CHEN Jia-lu,Wu Wei,HOU Kai*   

  1. College of Agronomy,Sichuan Agricultural University,Chengdu 611130,China
  • Online:2018-04-08 Published:2018-04-08

摘要: 白芷自古为美白常用药材,但近年因其香豆素类物质光敏毒性而被禁用于美白产品。本研究对比白芷不同提取部位美白活性及香豆素类物质含量,期望找到光敏物质含量低而美白效果较好的活性部位。采用酪氨酸酶抑制试验及人体美白试验测定白芷不同提取部位的美白活性;采用高效液相色谱法测定不同提取部位香豆素类物质含量。结果显示,白芷水提液比醇提液显示出较强的酪氨酸酶抑制作用,人体美白活性更高,而香豆素类物质含量较少。因此,白芷美白应选用其水溶性部位。

关键词: 白芷, 提取物, 酪氨酸酶活性抑制, 人体美白, 光敏毒性

Abstract: Angelicae Dahuricae is commonly used as whitening herb since ancient times,but it is disable as skin whitening product in recent years for coumarin in the root which has photosensitive toxicity.To find a better whitening active extractive with lower content of photosensitive toxicity,we compared the whitening activity of different extracts of this plant and measured their coumarin contents.Whitening activity of two extractions was determined by tyrosinase inhibition experiment and body whitening activity test,at the same time a high performance liquid chromatographic (HPLC) method was developed to determine the content of photosensitive coumarin.As a result,the water extraction showed a stronger inhibition on the activity of tyrosinase than the alcohol extraction did,and the body whitening activity of it was high.Photosensitive cumarin could barely be found in the water extraction but abundantly in the alcohol extraction.In conclusion,the water extraction of Angelicae Dahuricae was confirmed to be the active components responsible for whitening activity.

Key words: Angelicae Dahuricae, extraction, inhibitory effects on tyrosinase, skin whitening, photosensitive toxicity

中图分类号: 

R285.5