干燥方式对尾巨桉树皮提取物总三萜含量及其杀螺活性的影响

doi:10.16333/j.1001-6880.2019.9.023

天然产物研究与开发 ›› 2019, Vol. 31 ›› Issue (9): 1641-1646.doi: 10.16333/j.1001-6880.2019.9.023

干燥方式对尾巨桉树皮提取物总三萜含量及其杀螺活性的影响

李晶晶1*，甘金佳2，莫玲1，程浩1

1桂林医学院公共卫生学院，桂林 541004； 2中国科学院广西植物研究所，桂林 541006

出版日期:2019-10-09 发布日期:2019-10-23
基金资助:
国家自然科学基金（31760530）；广西自然科学基金（2016GXNSFAA380031）

Effects of drying methods on triterpenoids content and molluscicidal activity of Eucalyptus urophylla × Eucalyptus grandis bark extracts

LI Jing-jing1*,GAN Jin-jia2,MO Ling 1,CHENG Hao1

1School of Public Health,Guilin Medical University,Guilin 541004, China; 2Guangxi Institute of Botany,Chinese Academy of Sciences,Guilin 541006,China

Online:2019-10-09 Published:2019-10-23

摘要/Abstract

摘要： 为探讨干燥方式对尾巨桉树皮提取物总三萜含量的影响和其杀螺作用机理，实验以尾巨桉树皮为研究对象，比较鼓风干燥和冷冻干燥两种干燥方式对其树皮乙醇提取物总三萜含量及杀螺活性的影响。结果表明，当乙醇体积分数为60%时，鼓风干燥和冷冻干燥的尾巨桉树皮乙醇提取物中总三萜含量最高，分别为10.81%和13.90%。两种干燥方式得到的尾巨桉树皮乙醇提取物对福寿螺均有较强的毒杀活性，在50 mg/L的浓度下处理72 h，福寿螺的死亡率分别到达93.1%和100.00%；同时尾巨桉树皮提取物还能显著抑制福寿螺离水上爬，抑制效果优于同浓度下的对照药剂茶皂素（P < 0.05）。与空白对照组相比，经鼓风干燥的尾巨桉树皮提取物处理后，福寿螺头足和肝脏的蛋白质含量变化不大（P > 0.05）；经过冷冻干燥的尾巨桉树皮提取物处理后，福寿螺头足中蛋白质含量显著降低（P < 0.05），而肝脏中蛋白质含量无明显变化（P > 0.05）。鼓风干燥和冷冻干燥的尾巨桉树皮提取物均可有效抑制福寿螺头足部中LDH、AKP 和AST/GOT的活性（P < 0.05）以及肝脏中ALT/GPT和AST/GOT的活性（P < 0.05）。冷冻干燥的尾巨桉树皮提取物中三萜含量以及杀螺活性均高于鼓风干燥的树皮提取物。

关键词: 尾巨桉, 树皮, 干燥方式, 总三萜含量, 福寿螺, 杀螺活性

Abstract: Effects of drying methods on total triterpenoids content and molluscicidal activity of ethanol extracts from Eucalyptus urophylla × Eucalyptus grandis bark and its mechanism were studied.The results showed that when the volume fraction of ethanol was 60%,the total triterpenoids contents of hot air-dried and freeze-dried ethanol extracts of E. urophylla × E. grandis bark were the highest,with the total triterpenoids contents of 10.81% and 13.90%,respectively.The ethanol extracts of E. urophylla × E. grandis bark obtained by two drying methods possessed strong molluscicidal activity against P.canaliculata,with mortalities of 93.1% and 100.00% after 72 h,respectively,at a concentration of 50 mg/L.The extract of Eucalyptus bark also significantly inhibited climbing by P.canaliculatathe,and the inhibitory effect was better than that of tea saponin at the same concentration (P < 0.05).Compared to the control group,after treatment with the hot air-dried ethanol extracts of E. urophylla × E. grandis bark,the protein content in cephalopodium and liver of snails was no significant difference between them (P > 0.05).After treatment with the freeze-dried ethanol extracts of E. urophylla × E. grandis bark,significantly decreased the protein content in cephalopodium of snails compared with the control group (P < 0.05),while the protein content in the liver did not significantly change (P > 0.05).Both hot air dried and freeze-dried extracts of E. urophylla × E. grandis bark significantly decreased enzyme activities of LDH,AKP and AST/GOT in cephalopodium (P < 0.05) and ALT/GPT and AST/GOT in liver of the snails (P < 0.05).Higher triterpenoids content and molluscicidal activity were found in freeze-dried extracts of E. urophylla × E. grandis bark when compared to hot air-dried extracts.

Key words: Eucalyptus urophylla ×, Eucalyptus grandis;bark;drying;triterpenoids content;Pomacea canaliculata;molluscicidal activity

中图分类号:

R284

李晶晶1*，甘金佳2，莫玲1，程浩1. 干燥方式对尾巨桉树皮提取物总三萜含量及其杀螺活性的影响[J]. 天然产物研究与开发, 2019, 31(9): 1641-1646.

LI Jing-jing1*,GAN Jin-jia2,MO Ling 1,CHENG Hao1. Effects of drying methods on triterpenoids content and molluscicidal activity of Eucalyptus urophylla × Eucalyptus grandis bark extracts[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2019, 31(9): 1641-1646.

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干燥方式对尾巨桉树皮提取物总三萜含量及其杀螺活性的影响

Effects of drying methods on triterpenoids content and molluscicidal activity of Eucalyptus urophylla × Eucalyptus grandis bark extracts

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