天麻-附子祛风通络药对抗类风湿性关节炎风寒湿痹症大鼠的干预机制

doi:10.16333/j.1001-6880.2020.5.016

天然产物研究与开发 ›› 2020, Vol. 32 ›› Issue (5): 831-836.doi: 10.16333/j.1001-6880.2020.5.016

所属专题： No.5

天麻-附子祛风通络药对抗类风湿性关节炎风寒湿痹症大鼠的干预机制

郭步伐1，杨杰1*，彭启伦1，范光忠1，姚维一2，邓佳丽2，龚弟鸿2，丁维俊2

1毕节医学高等专科学校基础医学系，毕节 551700；2成都中医药大学基础医学院，成都 610075

出版日期:2020-05-28 发布日期:2020-06-16
基金资助:
贵州省科技计划（黔科合基础［2019］1003）

The intervention mechanism of Gastrodia elata Bl and Radix Aconiti Lateralis Preparata herb pairs (GRHP) on rats with rheumatoid arthritis and wind-cold-dampness arthralgia

GUO Bu-fa1,YANG Jie1*,PENG Qi-lun1,FAN Guang-zhong1,YAO Wei-yi2,DENG Jia-li2,GONG Di-hong2,DING Wei-jun2

1Department of Fundamental Medicine,Bijie Medical College,Bijie 551700,China;2Department of Fundamental Medicine,Chengdu University of Traditional Chinese Medicine,Chengdu 610075,China

Online:2020-05-28 Published:2020-06-16

摘要/Abstract

摘要： 阐释天麻-附子祛风通络药对抗类风湿性关节炎（RA）的作用机制。采用CIA法复制RA大鼠模型，并辅以风寒湿刺激条件。以关节炎指数≥4筛选RA大鼠模型，分为正常组、模型组以及天麻-附子药对各剂量配比组。ELISA法检测各组大鼠血清RF、CRP、TXB2、PGE2和PGI2的变化。RT-qPCR法检测各组大鼠全血COX-1和COX-2 mRNA表达的变化。结果表明：给药21天之后，与模型组相比，各剂量配比组大鼠关节炎指数下降，体重增加，明显降低血清RF、CRP、PGE2、PGI2水平，并能下调各组大鼠全血COX-2 mRNA的表达。因此，天麻-附子药对通过下调COX-2 mRNA表达，降低炎症因子PGE2和PGI2的含量而具有抗炎抗风湿作用。

关键词: 天麻-附子, 祛风通络药对, 类风湿性关节炎, 风寒湿痹症, 干预机制

Abstract:

To explain the mechanism of GRHP against rheumatoid arthritis (RA).RA rats’ model was constructed by collagen-induced arthritis (CIA) method and wind-cold-dampness stimulant condition,and screened by arthralgia index (AI) more than four.RA rats were classified to four groups such as model and three dose-ratio groups of GRHP except for normal group.Blood serum cytokine level of each rat was determined by ELISA approach,for example,rheumatoid factor (RF),C-reactive protein (CRP),thromboxane B2 (TXB2),prostaglandin E2 (PGE2) and prostacycline (PGI2).Whole blood COX-1 and COX-2 mRNA expression of each rats was determined by Real time quantitative PCR approach.As was shown in results,AI and blood serum cytokine level such as RF,CRP,PGE2 and PGI2 were markedly decreased,whole blood COX-2 mRNA expression reduced,body weight increased in the rats of dose-ratio groups by comparing with model group twenty-one day after intragastric administration.Therefore,GRHP has anti-inflammatory and anti-rheumatoid arthritis effects by downregulating COX-2 mRNA expression and decreasing proinflammatory factor level such as PGE2,PGI2,et al.

Key words: Gastrodia elata Bl and Radix Aconiti Lateralis Preparata, couplet medicines for dispelling wind and dredging collaterals, rheumatoid arthritis, wind-cold-dampness arthralgia, intervention mechanism.

中图分类号: R285.5

郭步伐, 杨杰, 彭启伦, 范光忠, 姚维一, 邓佳丽, 龚弟鸿, 丁维俊. 天麻-附子祛风通络药对抗类风湿性关节炎风寒湿痹症大鼠的干预机制[J]. 天然产物研究与开发, 2020, 32(5): 831-836.

GUO Bu-fa, YANG Jie, PENG Qi-lun, FAN Guang-zhong, YAO Wei-yi, DENG Jia-li, GONG Di-hong, DING Wei-jun.

The intervention mechanism of Gastrodia elata Bl and Radix Aconiti Lateralis Preparata herb pairs (GRHP) on rats with rheumatoid arthritis and wind-cold-dampness arthralgia

[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2020, 32(5): 831-836.

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天麻-附子祛风通络药对抗类风湿性关节炎风寒湿痹症大鼠的干预机制

The intervention mechanism of Gastrodia elata Bl and Radix Aconiti Lateralis Preparata herb pairs (GRHP) on rats with rheumatoid arthritis and wind-cold-dampness arthralgia

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