天然产物研究与开发 ›› 2022, Vol. 34 ›› Issue (1): 114-120.doi: 10.16333/j.1001-6880.2022.1.015

• 开发研究 • 上一篇    下一篇

灵芝麦角硫因高通量检测方法研究

徐晴元1，游俊健1，林俊芳1,2，薛玲娜1，余颖豪1，郭丽琼1,2*    

  1. 1华南农业大学食品学院生物工程系；2广东省微生态制剂工程技术研究中心，广州 510640
  • 出版日期:2022-01-28 发布日期:2022-01-28
  • 基金资助:
    国家自然科学基金（31772373，32072646）；广东省科技创新战略专项资金重点领域研发计划（2018B02020 5003）

A high-throughput detection method of ergothione in Ganoderma spp.

XU Qing-yuan1,YOU Jun-jian1,LIN Jun-fang1,2,XUE Ling-na1,YU Ying-hao1,GUO Li-qiong1,2*   

  1. 1College of Food Science,South China Agricultural University;2Research Center for Microecological Agent Engineering and Technology of Guangdong Province,Guangzhou 510640,China
  • Online:2022-01-28 Published:2022-01-28

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摘要： 麦角硫因（ergothioneine，EGT）基于其强抗氧化及体内消耗率低的特点，在人体内拥有多种重要的生理功能。但是在高产EGT微生物筛选和新菌株的选育研究中，现有的EGT检测方法因其步骤繁琐、使用的试剂和设备昂贵而亟待改进。本研究基于EGT的理化性质，建立了EGT-硫氰酸铁高通量快速检测体系，同时选用不同EGT产量的灵芝菌株以及灵芝融合新菌株对该检测体系的准确性进行验证。结果表明该方法可以快速准确地比较出样本间EGT产量高低，使原本需3~4天的工作缩短至2~3 h，HPLC验证结果显示，EGT-硫氰酸铁高通量快速检测体系效果良好，体系稳定。本研究结果将为高产EGT微生物的高通量筛选及高产EGT新菌株的选育提供新的方法和思路。

关键词: 麦角硫因, 高通量检测方法, 高效液相色谱, 灵芝

Abstract:

EGT (ergothioneine) has a variety of important physiological functions in human body because of its strong antioxidation and low consumption rate.However,the existing EGT detection methods are time-consuming and the reagents & equipments required are expensive in the screening of high-producing EGT microorganisms and the breeding of new strains.In this study,based on the physical and chemical properties of EGT,a high-throughput rapid detection system of EGT-ferric thiocyanate was established.Meanwhile,Ganoderma spp.strains with different EGT production and new Ganoderma spp. fusion strains were selected to verify the accuracy of the detection system.The results showed that this method can quickly and easily determine the difference of EGT yield between samples and shorten the detection time from original 3-4 days to present 2-3 hours.HPLC verification results show that the EGT- ferric thiocyanate high-throughput rapid detection system is effective and stable.The results of this study provided a new method for high-throughput screening of EGT-producing microorganisms and the breeding of new strains with high EGT production.

Key words: ergothioneine, high throughput detection method, high performance liquid chromatography, Ganoderma spp.

中图分类号:  Q93-31

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