天然产物研究与开发 ›› 2022, Vol. 34 ›› Issue (2): 239-244.doi: 10.16333/j.1001-6880.2022.2.009

• 研究简报 • 上一篇    下一篇

川芎内生菌Pseudeurotium ovale代谢产物研究

郭文秀，巨  凤，沈芋蓉，谭  璐，李新爱，郭大乐*，邓  赟*   

  1. 成都中医药大学药学院 西南特色中药资源国家重点实验室；中药材标准化教育部重点实验室，成都 611137
  • 出版日期:2022-02-28 发布日期:2022-03-07
  • 基金资助:
    国家自然科学基金面上项目（81973460）；四川省重点研发项目（2021YFN0134）；成都中医药大学杏林学者学科人才科研提升计划（BSH2018008）

Study on metabolites of Pseudeurotium ovale isolated from Ligusticum chuanxiong

GUO Wen-xiu,JU Feng,SHEN Yu-rong,TAN Lu,LI Xin-ai,GUO Da-le*,DENG Yun*   

  1. State Key Laboratory of Characteristic Chinese Medicine Resource of Southwest China;The Ministry of Education Key Laboratory of Standardization of Chinese Herbal Medicine,School of Pharmacy,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China
  • Online:2022-02-28 Published:2022-03-07

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摘要：

通过大孔树脂柱层析、凝胶柱层析和高效液相色谱等方法对川芎中分离得到的内生菌Pseudeurotium ovale的代谢物进行分离纯化，从中分离得到5个化合物，并通过核磁共振和质谱等波谱学技术手段确认所有化合物的结构，其中包含一个新的化合物4-methoxy-3-methyl-6-(1E,3E)-1,3-pentadien-1-yl-2H-pyran-2-one（1）和四个首次从中分离得到的已知化合物：(2E,4E)-1-(2,6-dihydroxy-3,5-dimethylphenyl)-2,4- hexadien-1-one（2）、2-deoxy-sohirnone C（3）、trichodimerol（4）、3,7-dihydroxy-1,9-dimethyl-dibenzofuran（5）。体外抗炎活性测试表明化合物1可抑制经脂多糖（LPS）处理的RAW 264.7细胞中NO、IL-6和TNF-α等炎症因子的分泌。

关键词: Pseudeurotium ovale, 代谢物, 抗炎活性

Abstract:

Five compounds,including a new one (1),were isolated from the broth of Pseudeurotium ovale,an endophytic fungus of Ligusticum chuanxiong by some column chromatographic such as macroporous resin column chromatography,gel column chromatography and HPLC.Their structures were elucidated as 4-methoxy-3-methyl-6-(1E,3E)-1,3-pentadien-1-yl-2H-pyran-2-one (1),(2E,4E)-1-(2,6-dihydroxy-3,5-dimethylphenyl)-2,4- hexadien-1-one（2）,2-deoxy-sohirnone C (3),trichodimerol (4),3,7-dihydroxy-1,9-dimethyl-dibenzofuran (5) by the interpretation of their spectroscopical evidences from 1D and 2D NMR as well as HR-ESI-MS.Compound 1 exhibited anti-inflammatory properties in vitro by attenuating the production of inflammatory mediators,such as nitric oxide (NO) as well as tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6).

Key words: Pseudeurotium ovale, metabolite, anti-inflammatory activity

中图分类号:  R284.1

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