天然产物研究与开发 ›› 2022, Vol. 34 ›› Issue (11): 1865-1870.doi: 10.16333/j.1001-6880.2022.11.007

• 研究简报 • 上一篇    下一篇

瑶药尖尾枫果实活性部位筛选及其化学成分研究

邹芷琪1,2，于玲玲2，潘争红1,2*，宁德生2，符毓夏2，李连春2   

  1. 1广西中医药大学药学院，南宁 530200；2广西壮族自治区中国科学院广西植物研究所广西植物功能物质与资源持续利用重点实验室，桂林 541006
  • 出版日期:2022-11-25 发布日期:2022-11-25
  • 基金资助:
    广西自然科学基金重点项目（2018GXNSFDA050016）；中央引导地方科技发展专项（桂科ZY20111010）；广西科学院基本科研业务费（CQZ-C-1901）

Study on active fraction screening and chemical constituents of Yao Medicine Callicarpa longissima fruits

ZOU Zhi-qi1,2,YU Ling-ling2,PAN Zheng-hong1,2 *,NING De-sheng2,FU Yu-xia2,LI Lian-chun2   

  1. 1College of Pharmacy,Guangxi University of Chinese Medicine,Nanning 530200,China;2Guangxi Key Laboratory of Plant Functional Phytochemicals and Sustainable Utilization, Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences,Guilin 541006, China
  • Online:2022-11-25 Published:2022-11-25

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摘要：

筛选尖尾枫果实的抗炎及抗氧化活性部位，并对抗炎成分进行研究。以NO生成抑制为筛选模型，并结合FRAP、ABTS及DPPH自由基清除法对尖尾枫果实的不同极性部位及化合物进行活性评价。采用硅胶、葡聚糖凝胶（Sephadex LH-20）、MCI柱色谱、高效液相色谱和重结晶等方法对活性部位进行分离纯化，并结合波谱学方法确定化合物结构。结果表明，尖尾枫果实乙酸乙酯萃取部位的抗炎及抗氧化活性均强于其他部位，并从中分离鉴定出9个化合物，包括2个黄酮：猫眼草黄素（1）和藿香黄酮醇（2）;7个二萜酚：鼠尾草酚（3）、迷迭香酚（4）、7-甲氧基迷迭香酚（5）、7-乙氧基迷迭香酚（6）、carnosic acid-11-methylether（7）、迷迭香双醛（8）、20-去氧鼠尾草酚（9），其中化合物3、4、6是其主成分，1、2、5~9均为首次从该植物中分离得到。化合物1~9均具有不同程度的抗炎作用，其中化合物3、4表现出良好的抗炎活性。

关键词: 尖尾枫, 抗炎, 抗氧化, 化学成分, 二萜酚

Abstract:

To screen the anti-inflammatory and anti-oxidative active fractions from fruits of Callicarpa longissima and study its chemical constituents.The activities of different fractions and compounds from fruits of C. longissima were evaluated by the inhibition of NO production and the multiple antioxidant methods including FRAP,ABTS and DPPH radical scavenging.The compounds were isolated and purified from the active fractions by using various chromatography techniques including silica gel,Sephadex LH-20,MCI,HPLC and recrystallization,and their structures were identified by spectroscopic methods.The results showed that the anti-inflammatory and anti-oxidative activities of the ethyl acetate fraction were stronger than others.Nine compounds,obtained from active fractions,were identified as two flavonoids:chrysosplenetin (1) and pachypodol (2);and seven diterpene phenols:carnosol (3),rosmanol (4),7-methoxyrosmanol (5),7-ethoxyrosmanol (6),carnosic acid-11-methylether (7),rosmadial (8),20-deoxocarnosol (9),respectively.Compounds 3,4,6 were the main compounds,and 1,2,5-9 were isolated from this plant for the first time.Compounds 1-9 have anti-inflammatory effects,among which compounds 3, 4 showed significant anti-inflammatory activity.

Key words: Callicarpa longissima, anti-inflammatory, anti-oxidative, chemical constituents, diterpene phenols

中图分类号:  R284.1

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