天然产物研究与开发 ›› 2023, Vol. 35 ›› Issue (增刊2): 46-54.

• 研究简报 • 上一篇    下一篇

中国被毛孢亚临界水提取多糖结构特征及抗氧化活性

代千千1,2,3,胡   娜1,2,王洪伦1,2,丁晨旭1,2*   

  1. 1中国科学院西北高原生物研究所 中国科学院藏药研究重点实验室;2青海省藏药研究重点实验室,西宁 810008;3中国科学院大学,北京 100049
  • 出版日期:2023-12-27 发布日期:2023-12-11

Structural characteristic and anti-oxidant activity of polysaccharide from Hirsutella sinensis extracted by subcritical water

DAI Qian-qian1,2,3,HU Na1,2,WANG Hong-lun1,2,DING Chen-xu1,2*   

  1. 1Key Laboratory of Tibetan Medicine Research,Northwest Institute of Plateau Biology,Chinese Academy of Sciences;2Key Laboratory of Tibetan Medicine Research of Qinghai Province,Xining 810008,China;3University of the Chinese Academy of Sciences,Beijing 100049,China
  • Online:2023-12-27 Published:2023-12-11

摘要: 本实验以中国被毛孢菌粉为原料,通过响应面实验对亚临界水提取中国被毛孢多糖的工艺进行优化,并对提取的多糖进行结构分析和抗氧化活性研究,以开发先进的中国被毛孢多糖的提取方法,从而为中国被毛孢的合理开发和应用提供理论依据。结果表明,亚临界水提取最佳工艺条件为温度160 ℃、时间35 min、流速3.5 mL/min,此条件下多糖提取率为18.32%。体外抗氧化活性实验结果表明,亚临界水提取中国被毛孢多糖具有一定的抗氧化能力。该研究结果表明亚临界水提取是一种潜在的多糖提取方式,可获得高产率和良好抗氧化能力的中国被毛孢多糖。

关键词: 中国被毛孢, 多糖, 亚临界水提取, 抗氧化, 结构特征

Abstract:

The extraction condition of polysaccharide from Hirsutella sinensis by subcritical water was optimized using the response surface experiment.Then,analyze the structure and antioxidant activity of polysaccharide to provide theoretical basis for the rational development and application of H. sinensis polysaccharide.The optimal extraction conditions were that the extraction temperature was 160 ℃,the time was 35 min and the extraction flow rate was 3.5 mL/min,and the extraction rate of polysaccharide was up to 18.32% The study on antioxidant revealed that the polysaccharide had certain antioxidant activity. The results indicate that subcritical water extraction was a potential method to extract polysaccharides from H. sinensis with high yield and good antioxidant capacity.

Key words: Hirsutella sinensis, polysaccharide, subcritical water extraction, anti-oxidant, structural characteristics

中图分类号:  R284.2