To study the chemical constituents of Pleurotus tuoliensis and their biological activities.ODS,silica gel column chromatography,semi-preparative high performance liquid chromatography and other methods were used to isolate and purify compounds,and modern spectrum technologies together with physical and chemical methods were applied to identify the structures of those isolated compounds.As a result,11 compounds were isolated from the extract of P. tuoliensis for the first time,named fusarpyrones A (1),5-hydroxydihydrofusarium C (2),3-methyl ether fusarium (3),2-acetonyl-3-methyl-7-methoxy-naphthazarin (4),(-)-cyclonerodiol (5),2-isopropanol-3-methyl-7-methoxy-naphthazarin (6),1,2-dihydroxymentolactone (7),6,7-dihydroxy-3,6-dimethyl-2-isoval eroyl-4,5,6,7-tetrahydrobenzo furan (8),dihydro-3-epi-pleurolactone (9),5-hydroxymethyl furfural (10),6,7-dihydroxy-3,6-dimethyl-2-isovaleroyl-4,5,6,7-tetrahydrobenzofuran (11).Furthermore,all the compounds were screened for anti-cervical cancer activity on Hela cells.The activity results demonstrated that compounds 5,9 and 10 displayed weak inhibitory activity,other compounds showed stronger effects,and compound 3 exhibited the best activity with the IC₅₀ value of 3.78 μmol/L,which has the value for further research.