天然产物研究与开发 ›› 2025, Vol. 37 ›› Issue (3): 537-543.doi: 10.16333/j.1001-6880.2025.3.017 cstr: 32307.14.1001-6880.2025.3.017

• 开发研究 • 上一篇    下一篇

楝酰胺对急性髓系白血病细胞线粒体功能的影响

邓 娜1,2，高议雁1,2，李佳瑞1,2，张 镒1,2，李君兰1,3,4，周佳钰1,2，宋佳蕾1,3,4*   

  1. 1贵州中医药大学基础医学院；2贵州中医药大学药学院;3贵州中医药大学 分子生物学重点实验室；4贵州中医药大学 分子诊断研究中心，贵阳 550025
  • 出版日期:2025-04-01 发布日期:2025-04-01
  • 基金资助:
    国家级大学生创新创业训练计划(2024106620606)；贵州省大学生创新创业训练计划(S2024106621522)；贵州省科技计划项目(黔科合基础-ZK［2022］一般485)；贵州省教育厅青年科技人才成长项目(黔教合KY字［2022］267号)

Effect of rocaglamide on mitochondria function in acute myeloid leukemia cells

DENG Na1，2,GAO Yi-yan1，2,LI Jia-rui1，2, ZHANG Yi1，2,LI Jun-lan1,3,4,ZHOU Jia-yu1，2,SONG Jia-lei1,3,4*   

  1. 1School of Basic Medicine,Guizhou University of Traditional Chinese Medicine;2School of Pharmacy,Guizhou University of Traditional Chinese Medicine;3The Key Laboratory of Molecular Biology,Guizhou University of Traditional Chinese Medicine;4Molecular Diagnosis Research Center,Guizhou University of Traditional Chinese Medicine,Guiyang 550025,China

  • Online:2025-04-01 Published:2025-04-01

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摘要：

探讨楝酰胺对急性髓系白血病(acute myeloid leukemia，AML)细胞线粒体的作用。MTT法测定楝酰胺对AML细胞HEL生长增殖的影响，PI法测定细胞周期的变化。流式细胞技术分析楝酰胺对细胞凋亡率的影响，Western blot检测线粒体凋亡途径相关蛋白的变化，钙黄绿素法分析楝酰胺对线粒体通透性转换孔(mitochondrial permeability transition pore，mPTP)的影响，JC-1染料检测线粒体膜电位的变化，荧光素酶实验测定三磷酸腺苷(adenosine triphosphate，ATP)水平。结果显示楝酰胺抑制HEL细胞的生长增殖并呈现时间和浓度依赖性，阻滞细胞周期于G0/G1期。楝酰胺诱导HEL细胞线粒体途径凋亡，激活HEL细胞mPTP的开放，降低了线粒体膜电位和ATP水平。以上结果说明，楝酰胺改变了HEL细胞的线粒体功能，从而抑制细胞增殖。

关键词: 楝酰胺, 急性粒细胞白血病, 线粒体通透性转换孔, 线粒体膜电位, 凋亡

Abstract:

This study aims to explore the effect of rocaglamide on mitochondria function in acute myeloid leukemia (AML) cell line HEL.MTT assay was used to determine the effect of rocaglamide on the proliferation of HEL cells.Cell cycle was analyzed by PI staining.Flow cytometry was used to detect the apoptotic rate of HEL cells.Western blot was used to detect mitochondrial apoptosis pathway related protein.The effect of rocaglamide on mitochondrial membrane permeability was analyzed by calcein method.JC-1 dye was used to detect the changes in mitochondrial membrane potential.Luciferase experiment was used to determine the effect of rocaglamide on adenosine triphosphate(ATP) level.The results showed that rocaglamide inhibited the proliferation of HEL cells in a time-and concentration-dependent manner and arrested cell cycle of HEL cells at G0/G1 phase.Rocaglamide induced mitochondrial pathway apoptosis,activated the opening of the mitochondrial permeability transition pore(mPTP),and reduced the mitochondrial membrane potential of HEL cells as well as the ATP level.The above results indicated that rocaglamide remodeled the mitochondrial function of HEL cells,thereby inhibiting the proliferation of AML cells.

Key words: rocaglamide, acute myeloid leukemia, mitochondrial permeability transition pore, mitochondrial membrane potential, apoptosis

中图分类号:  R932

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