天然产物研究与开发 ›› 2025, Vol. 37 ›› Issue (11): 2113-2119.doi: 10.16333/j.1001-6880.2025.11.015 cstr: 32307.14.1001-6880.2025.11.015

• 开发研究 • 上一篇    下一篇

洛克米兰醇调控Cyclin D1抑制乳腺癌细胞增殖的研究

周佳钰2†，李佳瑞2†，高议雁2，李君兰1,3，孙丽娥4，苑春茂5,6*，宋佳蕾1,3*   

  1. 1贵州中医药大学基础医学院；2贵州中医药大学药学院；3贵州中医药大学分子生物学重点实验室；4贵州中医药大学第二附属医院；5贵州省天然产物研究中心；6贵州医科大学省中药功效成分发掘与利用全国重点实验室，贵阳 550000
  • 出版日期:2025-11-27 发布日期:2025-11-26
  • 基金资助:
    国家级大学生创新创业训练计划(2024106620603)；贵州省大学生创新创业训练计划(S2024106621522)；贵州省科技计划(黔科合基础-ZK［2022］一般485)；国家自然科学基金地区项目(82260854)

Rocaglaol inhibits the proliferation of breast cancer cells by regulating Cyclin D1 

ZHOU Jia-yu2†,LI Jia-rui2†,GAO Yi-yan2,LI Jun-lan1,3,SUN Li-e4,YUAN Chun-mao5,6*,SONG Jia-lei1,3*   

  1. 1School of Basic Medicine,Guizhou University of Traditional Chinese Medicine;2School of Pharmacy,Guizhou University of Traditional Chinese Medicine;3The Key Laboratory of Molecular Biology,Guizhou University of Traditional Chinese Medicine;4The Second Affiliated Hospital,Guizhou University of Traditional Chinese Medicine;5Natural Products Research Center of Guizhou Province;6State Key Laboratory of Discovery and Utilization of Functional Components in Traditional Chinese Medicine,Guizhou Medical University,Guiyang 550000,China
  • Online:2025-11-27 Published:2025-11-26

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摘要： 探讨洛克米兰醇(rocaglaol，Roc)通过调节Cyclin D1影响乳腺癌细胞增殖的作用及机制。采用不同浓度的Roc处理人乳腺癌细胞MCF7和MDA-MB-231，MTT法检测Roc对细胞增殖的影响；流式细胞术测定细胞周期的变化；Western blot分析Roc对细胞周期蛋白D1(Cyclin D1)的影响，PCR和q-PCR检测CCND1基因的表达情况；蛋白酶体抑制剂MG132检测Roc调节Cyclin D1的方式。结果表明，Roc抑制人乳腺癌细胞MCF7和MDA-MB-231的增殖，阻滞细胞周期于G2/M期，下调了MCF7和MDA-MB-231细胞Cyclin D1的蛋白表达，但不影响CCND1基因的表达。MG132能够恢复Roc对Cyclin D1表达下调的情况，表明Roc以蛋白酶体降解的方式调节Cyclin D1的表达。以上结果说明Roc在转录后水平调节Cyclin D1的表达，从而抑制乳腺癌细胞的增殖。

关键词: 洛克米兰醇, 乳腺癌, 细胞周期, Cyclin D1, 转录后调节

Abstract:

The purpose of this study was to investigate the mechanism of rocaglaol (Roc) on the proliferation of breast cancer via Cyclin D1.Different concentrations of Roc were treated with human breast cancer cells MCF7 and MDA-MB-231.MTT assay were used to detect the effects of Roc on the cell proliferation.Flow cytometry technology determined the cell cycle distribution affected by Roc.Western blot analyzed the effects of Roc on Cyclin D1,as well as PCR and q-PCR were used to detect the effects of Roc on the CCND1 gene.Protease inhibitor MG132 was used to detect the way how Roc regulated Cyclin D1.The results showed that Roc inhibited the proliferation of human breast cancer cells MCF7 and MDA-MB-231,arrested the cell cycle at the G2/M phase,and down-regulated the protein expression of Cyclin D1,but did not affect the expression of CCND1 gene.MG132 could restore the decrease of Cyclin D1 expression induced by Roc,indicating that Roc regulates the expression of Cyclin D1 by proteasome degradation.The above results indicate that Roc decreases the expression of Cyclin D1 at post-transcriptional level,thereby inhibiting the proliferation of breast cancer cells.

Key words: rocaglaol, breast cancer, cell cycle, Cyclin D1, post-transcriptional regulation

中图分类号:  R285.5

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