野生坚龙胆及其组培苗中龙胆苦苷的含量分析

天然产物研究与开发 ›› 2011, Vol. 23 ›› Issue (3): 482-485.

野生坚龙胆及其组培苗中龙胆苦苷的含量分析

朱宏涛1,2，郑传伟2，赵平2,3，李元1,4，杨崇仁2，张颖君2*

1云南农业大学植物保护学院，昆明 650201；2中国科学院昆明植物研究所植物化学与西部植物资源持续利用国家重点实验室，昆明 650204；3西南林学院资源学院，昆明 650224；4云南农业大学资源与环境学院，昆明 650201

收稿日期:2009-09-25 出版日期:2011-06-25 发布日期:2011-08-08
通讯作者: zhangyj@mail.kib.ac.cn

Contents Analysis of Gentiopicroside in Wild and Tissue Culture Seedlings of Gentiana rigescens

ZHU Hong-tao1,2, ZHENG Chuan-wei2, ZHAO Ping2,3, LI Yuan1,4, YANG Chong-ren2, ZHANG Ying-jun2*

1College of Plant Protection, Yunnan Agricultural University, Kunming 650201,China; 2State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, China; 3Faculty of Resources, Southwest Forestry University, Kunming 650224, China; 4College of Resources and Environment, Yunnan Agricultural University, Kunming 650201,China

Received:2009-09-25 Online:2011-06-25 Published:2011-08-08

摘要/Abstract

摘要：

利用含有α-萘乙酸（NAA）、6-苄氨基嘌呤（6-BA ）和激动素（KT）等激素的MS固体培养基，对野生坚龙胆进行组培苗诱导培养。同时，应用高效液相色谱技术对野生坚龙胆和其组培苗的根、茎和叶中的龙胆苦苷进行含量分析和比较研究。结果发现，野生坚龙胆中的龙胆苦苷主要储存于根部，而组培坚龙胆中龙胆苦苷在根中的含量甚微，主要集中在茎、叶部位，且在出根初期组培坚龙胆叶中的龙胆苦苷含量近似于或略大于野生龙胆根中的含量。研究结果提示，坚龙胆中的绿色组织是龙胆苦苷合成的部位，这些部位同时具有龙胆苦苷的储藏功能。

Abstract:

Tissue culture seedlings of Gentiana rigescens were carried out in the MS’s media with NAA, 6-BA and KT. The contents of gentiopicroside in leaves, stems and roots of tissue culture seedlings and wild plant were analyzed by HPLC. It was noticed that plentiful gentiopicroside accumulates in roots of wild plant, but hardly in roots of tissue culture seedlings. However, the content of gentiopicroside in leaves of tissue culture seedlings was higher or almost same as that in the wild roots. The results suggested that gentiopicroside was synthesized in the leaves and stored in the leaves simultaneously.

Key words: Gentiana rigescens, gentiopicroside, HPLC analysis

中图分类号:

Q946

朱宏涛，郑传伟，赵平，李元，杨崇仁，张颖君. 野生坚龙胆及其组培苗中龙胆苦苷的含量分析[J]. 天然产物研究与开发, 2011, 23(3): 482-485.

ZHU Hong-tao, ZHENG Chuan-wei, ZHAO Ping, LI Yuan, YANG Chong-ren, ZHANG Ying-ju. Contents Analysis of Gentiopicroside in Wild and Tissue Culture Seedlings of Gentiana rigescens[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2011, 23(3): 482-485.

参考文献 14

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