闪提技术提取罗汉果甜甙

天然产物研究与开发 ›› 2011, Vol. 23 ›› Issue (3): 561-564.

闪提技术提取罗汉果甜甙

刘兆1，荣永海1，王志滨2，荣龙1*

1北京航空航天大学生物与医学工程学院，北京 100191；2广西北航工程技术研究院，南宁 530022

收稿日期:2010-01-06 出版日期:2011-06-25 发布日期:2011-08-08
通讯作者: ronglong@buaa.edu.cn
基金资助:
广西科学技术厅自然科学基金项目（0728259）

Extraction of Mogrosides by using Flash extraction technique

LIU Zhao1, RONG Yong-hai1, WANG Zhi-bin2, RONG Long1*

1 School of Biological Science and Medical Engineering,Beihang University, Beijing 100191, China; 2Guangxi-Beihang Institute of Engineering and Technology, Nanning 530022, China

Received:2010-01-06 Online:2011-06-25 Published:2011-08-08

摘要/Abstract

摘要：

通过比较超声法、微波法及闪提法提取罗汉果甜甙的效率，发现闪提法具有较好的提取效果。通过正交试验优选得到罗汉果甜甙的最佳闪提条件为：料液比1:20、转速6000 r/min、常温下提取4 min。最佳条件下甜甙提取率为10.057%，说明闪式提取技术是一种高效和低成本的提取方法。

关键词: 闪提法, 微波法, 超声法, 正交设计, 罗汉果甜甙

Abstract:

The flash extract method was more effective for mogrosides extraction by comparing extract efficiencies of ultrasonic method, microwave method, and flash method. Flash extraction conditions were determined by an orthogonal test design, and the optimum extraction conditions were as follows: added 20 times water and extracted for 4 min at room temperature with 6000 r/min. In these conditions, the extraction efficiency of mogrosides was 10.057%, which indicated that the flash extraction was an efficient and low-cost method.

Key words: flash extraction, microwave extraction, ultrasonic extraction, orthoganal experiment, mogrosides

中图分类号:

Q946.91 R284

刘兆，荣永海，王志滨，荣龙. 闪提技术提取罗汉果甜甙[J]. 天然产物研究与开发, 2011, 23(3): 561-564.

LIU Zhao, RONG Yong-hai, WANG Zhi-bin, RONG Long. Extraction of Mogrosides by using Flash extraction technique[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2011, 23(3): 561-564.

参考文献 12

1	中华人民共和国卫生部药典委员会).PRC codex(中华人民共和国药典).Beijing: Chemical Industry Press,1977.348;1985.181;1990.184;1997.349.Chinese Pharmacopoeia Commission(
2	高山林),Wang H(汪红).The technique onextraction and content determination of saponin from Momordica Grosvenori.Nat Prod Res Dev(天然产物研究与开发),2001,13(02): 36-40.Gao SL(
3	陈迪华),Si JY(斯建勇),et al.Studies and uses of natural non-sugar Sweeteners from Luo-hanguo(Fruit of Siraitia Grosvenori).Nat Prod Res Dev(天然产物研究与开发),1992,04(01):72-77.Chen DH(
4	马少妹),Yuan AQ(袁爱群),et al.Studies on ultrasonic enhanced extraction technology of mogroside.J Shenyang Pharm Univ(沈阳药科大学学报),2006,23(5):316-319.Ma SM(
5	杨祖金),Zhong L(钟理),et al.Study on the Technique of Ultrasonic Wave Extraction of Triterpeneclucoside.Chin Tradit Herb Drugs(中草药),2007,30(08): 1019-1021.Yang ZJ(
6	朱晓韵),He CW(何超文).Application of microwave technique to extraction of Mogroside from fresh luohanguo.Guangxi·Light Ind(广西轻工业),2002,(02):11-13.Zhu XY(
7	黎海彬),Li L(李琳),Hu SQ(胡松青).et al.Study on microwave-assisted extraction of triterpene glucoside from Siraitia grosvenorii.Food Sci(食品科学),2003,24(02):92-95.Li HB(
8	刘延泽).Principle and practice of smashing tissue extraction and herbal blitzkrieg extractor.Chin J Nat Med(中国天然药物),2007,5(06):406-408.Liu YZ(
9	周湛),Liu YZ(刘延泽),Liu GL(刘改岚),et al.Studies on the smashing tissue extraction and purification process of the total sapònins of the basal part of stem and beard from Panax notoginseng.Mod Chin Med(中国现代中药),2009,11:34-36.Zhou Z(
10	汪中博),Zhao YQ(赵余庆).Studies on the smashing tissue extraction process of the total saponins of Centella Asiatica.Mod Chin Med(中国现代中药),2009,11(2):36-38.Wang ZB(
11	Lee CH.Intense sweetener from Lo Han Kuo (Momordica grosvenorii).Experientia,1975,31:533
12	Matsumoto K,Kasai R,Ohtani K,et al.Minor cucurbitane glycosides from fruits of Siraitia grosvenorii(Cucurbitancaae).Chem Pharm Bull,1990,38(7):2030.

[1]	何宝峰, 马新换, 马忠祥, 王宝才, 徐志伟, 马芳兄, 杨天艳, 李荣焜. 基于改进熵权法结合TOPSIS模型和BPNN建模优化甘草多糖醇沉工艺[J]. 天然产物研究与开发, 2023, 35(2): 271-280.
[2]	魏思敏1，王英辉2，唐志书1，苏瑞1，许洪波1，陈琳1，刘世军1，李琛1 . 大枣水提液还原制备纳米银材料及抗氧化和抗菌活性研究[J]. 天然产物研究与开发, 2020, 32(2): 182-190.
[3]	苗永美*，孙佳琦，徐荣华，汪雁. 广东石豆兰多糖的提取工艺及其抗氧化活性[J]. 天然产物研究与开发, 2019, 31(5): 779-785.
[4]	李刚1 , 魏庆红1* , 段伟伟1. 一种新的连翘中连翘苷的HPLC含量测定方法[J]. 天然产物研究与开发, 2018, 30(增刊1): 137-140.
[5]	张喜峰1,2*，陈雨迪1，崔晶1，程雯慧1. 锁阳原花青素提取方法及抗氧化和抗糖基化研究[J]. 天然产物研究与开发, 2018, 30(12): 2039-2048.
[6]	彭练慈，殷中琼*，康帅，曲径，刘明辉，陈萍. 黄芩等三味具有抑菌活性中药的最佳提取工艺研究[J]. 天然产物研究与开发, 2015, 27(7): 1273-1279.
[7]	李芸达1,颜祖弟1,黄涛1,张照平1,黄莹1,李远辉1,黄锁义2*. 王不留行多糖的提取工艺及其抗氧化活性研究[J]. 天然产物研究与开发, 2015, 27(3): 446-450.
[8]	康帅1，殷中琼1*，贾仁勇2,3，彭练慈1，曲径1，梁陈1. 乌梅等三味具有抑菌活性中药的最佳提取工艺研究[J]. 天然产物研究与开发, 2014, 26(12): 2014-2021.