关键词: 糖蜜草, 固相微萃取, 气质联用, 挥发性成分

Abstract: In this study, a head-space solid phase microextraction (HS-SPME) method was developed for extracting the volatile compounds from Melinis minutiflora. Then gas chromatography-mass spectrometry (GC-MS) coupled with retention indicator and comparison with standard samples was used to characterize the components of the volatile compounds. Then the relative proportion of each component was calculated by the peak area normalization method. The results showed that Polydimethylsiloxane/divinybenzene(PDMS/DVB) SPME fibers based on the number of peaks performed well for extracting volatile compounds from M. minutiflora. Thirty-three compounds were obtained which accounted for 90.94% of the total peak areas with alkanes and terpenes as the dominant components. This study lays the foundation for developing M. minutiflora as the repellent plant for rice stem borer in terms of effective pest prevention. Also, it is the ground work for applying the evading volatiles to the rice field.

Key words: Melinis minutiflora, SPME, GC-MS, volatile constituents