关键词: HPLC指纹图谱, 枫蓼肠胃康口服液, 牛耳枫, 辣蓼

Abstract: This study established a HPLC HPLC–fingerprint method for the general characterization and indicator component determination. This method was used to investigate the HPLC fingerprint of Fengliao Changweikang (FC) oral liquid comprising of Daphniphyllum calycinum Benth. and Polygonum hydropiper Linn. The contents of rutin, hyperin and isorhamnetinn as the indicator components were determined.The analysis was performed on a waters C₁₈ (250 mm×4.6 mm I.D. 5 µm) utilizing a gradient elution profile with methanol and formic acid as the mobile phase. The results showed that calibration curves were linear with correlation coefficients >0.999 for all analytes.In accordance with the State Food and Drug Administration (SFDA) requirements, similarity degree was calculated by both correlation coefficient and the included angle cosine to verify the results.The similarity degree were more than 0.982 which suggested that the established HPCL –fingerprint method is significantly improved compared with the traditional method in characterizing and quantifying the components of Fengliao Changweikang (FC) oral liquid.

Key words: HPLC–fingerprint, FC oral liquid, Daphniphyllum calycinum Benth., Polygonum hydropiper Linn