褐蘑菇水溶性多糖抗氧化活性研究

doi:A

天然产物研究与开发 ›› 2012, Vol. 23 ›› Issue (3): 374-377.doi: A

褐蘑菇水溶性多糖抗氧化活性研究

李芳亮^1*，王锐¹，刘莹¹，高杨²，赵立冬²，张凤如¹，韩锡赢¹

¹辽宁工程技术大学理学院，阜新 123000；²辽宁省阜新市科学技术局，阜新 123000

收稿日期:2010-12-08 出版日期:2012-03-26 发布日期:2012-03-31

Antioxidant Activity of Water-soluble Polysaccharides Isolated from Agaricus bisporus

LI Fang-liang^1*, WANG Rui¹, LIU Ying¹, GAO Yang², ZHAO Li-dong², ZHANG Feng-ru¹, HAN Xi-ying¹

¹Liao Ning Technical University, College of Science, Fuxin 123000, China; ²Fuxin Science and Technology Bureau, Fuxin 123000, China

Received:2010-12-08 Online:2012-03-26 Published:2012-03-31
Supported by:
This work was supported by the Excellent youth foundation of Liaoning Technical University（07-127）

摘要/Abstract

摘要： 采用水提醇沉以及sevag去蛋白的方法获得褐蘑菇水溶性多糖（WPPA）。通过测定还原力、超氧阴离子清除率、羟基自由基清除率和抑制H2O2诱导红细胞氧化溶血实验评价WPPA抗氧化活性。结果表明：WPPA具有较强的还原力，对•O2−和•OH具有较强的清除作用，IC50分别为527 μg/mL、310 μg/mL；对H2O2诱导红细胞氧化溶血及MDA生成有很强的抑制作用，IC50分别为700 μg/mL和541 μg/mL。说明WPPA在一定浓度内具有较强的抗氧化能力。

关键词: 褐蘑菇, 多糖, 抗氧化, 红细胞溶血

Abstract: The antioxidant effects of Water Soluble Polysaccharides extracted from Portabella Mushroom Agaricus bisporus (WPPA) were studied by in vitro four different experiments: measuring reducing power, eliminating superoxide anion free radical experiment, eliminating hydroxyl free radical experiment, inhibiting mice erythrocyte hemolysis (MEH) and lipid peroxidation MDA induced by H2O2 experiment. The reducing power of WPPA was excellent. The scavenging abilities of superoxide radical and hydroxyl free radical were notable with IC50 value of 527 and 310 μg/mL, respectively. In addition, WPPA exhibited significant inhibitory actions on MEH and formation of MDA mediated by H2O2 with IC50 value of 700 and 541 μg/mL, respectively. The data obtained in the models in vitro clearly demonstrated the antioxidant potency of the WPPA.

Key words: Agaricus bisporus, polysaccharides, antioxidant activity, erythrocyte hemolysis

中图分类号:

Q946.91 R285.5

李芳亮，王锐，刘莹，高杨，赵立冬，张凤如，韩锡赢. 褐蘑菇水溶性多糖抗氧化活性研究[J]. 天然产物研究与开发, 2012, 23(3): 374-377.

LI Fang-liang, WANG Rui, LIU Ying, GAO Yang, ZHAO Li-dong, ZHANG Feng-ru, HAN Xi-ying. Antioxidant Activity of Water-soluble Polysaccharides Isolated from Agaricus bisporus[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2012, 23(3): 374-377.

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褐蘑菇水溶性多糖抗氧化活性研究

Antioxidant Activity of Water-soluble Polysaccharides Isolated from Agaricus bisporus

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