响应面法优化巨大芽孢杆菌中的聚-β-羟基丁酸（PHB）提取工艺

doi:A

天然产物研究与开发 ›› 2012, Vol. 23 ›› Issue (3): 393-397.doi: A

响应面法优化巨大芽孢杆菌中的聚-β-羟基丁酸（PHB）提取工艺

周琴，董夏梦，汪鹏荣，蓝丽精，嵇豪，蒋冬花*

浙江师范大学化学与生命科学学院，金华 321004

收稿日期:2010-10-15 出版日期:2012-03-26 发布日期:2012-03-31
基金资助:
浙江省公益性技术应用研究计划项目（NO. 2010C32076），浙江省自然科学基金项目（NO.Y3090343），浙江省大学生科技创新活动计划（NO.2010R404064）。

Optimal Extraction Conditions of Poly-β-hydroxybutyrate (PHB) in Bacillus megaterium by Response Surface Methodology

ZHOU Qin, DONG Xia-meng, WANG Peng-rong, LAN Li-jing, JI Hao, JIANG Dong-hua*

College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, China

Received:2010-10-15 Online:2012-03-26 Published:2012-03-31

摘要/Abstract

摘要： 为获得聚-β-羟基丁酸（PHB）提取的最佳工艺，以氯仿浓度，提取时间，和提取温度为影响因子，在单因素实验结果的基础上，应用Box-Benhnken中心组合方法进行三因素三水平的实验设计，以PHB提取量为响应值，运用响应面法（RSM）对提取条件进行进一步的优化。结果表明：在氯仿浓度69.7%，提取温度59.7 ℃，提取时间3.0 h的条件下，PHB提取量达到最大值4.430 g/L。

关键词: 巨大芽孢杆菌（Bacillus megaterium）；聚-&beta, -羟基丁酸（PHB）；响应面法（RSM）

Abstract: To obtain the optimal extraction conditions of Poly-β-hydroxybutyrate (PHB) in Bacillus megaterium, the effect of chloroform concentration, extraction time and temperature on PHB extraction were studied. On the basis of single factor experiment, Box-Benhnken central composite method was designed for the three factors. In response to PHB extraction value, the extraction conditions were further optimized by response surface method (RSM). The results showed that when chloroform concentration was 69.7%, extraction temperature was 59.7 ℃, extraction time was 3.0 h, the productivity of PHB was 4.437 g/L, reached its maximum.

Key words: Bacillus megaterium, poly-β-hydroxybutyrate (PHB), response surface methodology (RSM)

中图分类号:

Q939.97

周琴，董夏梦，汪鹏荣，蓝丽精，嵇豪，蒋冬花. 响应面法优化巨大芽孢杆菌中的聚-β-羟基丁酸（PHB）提取工艺[J]. 天然产物研究与开发, 2012, 23(3): 393-397.

ZHOU Qin, DONG Xia-meng, WANG Peng-rong, LAN Li-jing, JI Hao, JIANG Dong-hua. Optimal Extraction Conditions of Poly-β-hydroxybutyrate (PHB) in Bacillus megaterium by Response Surface Methodology[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2012, 23(3): 393-397.

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响应面法优化巨大芽孢杆菌中的聚-β-羟基丁酸（PHB）提取工艺

Optimal Extraction Conditions of Poly-β-hydroxybutyrate (PHB) in Bacillus megaterium by Response Surface Methodology

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