关键词: 东北红豆杉, 多糖, HPLC, GC-MS

Abstract: TP was obtained by water extraction and alcohol precipitation from Taxus cuspidate. TP was filtrated through a cellulose ultrafiltration system to give the extra-ultrafiltrate with TP-1 and intro-ultrafiltrate with TP-2. TP-2 was treated with partially acid hydrolysis, then purified by Sephadex G-75 column to obtain the polysaccharides TP-2-1a. TP-2-1a was studied, including the physical and chemical properties, molecular weight, sugar compositions, glycosidic linkage analysis and so on. The molecular weight of TP-2-1a was about 7.0×103 kDa. Suger compositions were Rha, Man, Gal, Glu, GalA and GlcA, in molar ratio of 116.9:1:15.5:1.3:9.9:2.5. The backbone of TP-2-1a were (1→3) and (1→4)-linked galactosyl, (1→4) and (1→6)-linked mannosyl, (1→3) and (1→4)-linked glucosyl, and (1→4)-linked galacturonide, There were side chains attached to O-6 of (1→3)-linked galactoside and O-4 of (1→2)-linked rhamnoside. And the non-reducing terminals were mostly galactose and a few mannose, glucose and rhamnose. This is the first time to obtain the polysaccharides from T. cuspidate.

Key words: Taxus cuspidate, polysaccharides, HPLC, GC-MS