富硒灵芝调控大鼠乙酰辅酶A羧化酶α表达治疗非酒精性脂肪肝病的研究

天然产物研究与开发 ›› 2014, Vol. 26 ›› Issue (7): 1086-1092.

富硒灵芝调控大鼠乙酰辅酶A羧化酶α表达治疗非酒精性脂肪肝病的研究

武俊紫1,2，贾亚敏3，沈平瑞4，胡跃高5，全胜麟2，李燕2*，李树德6*

1 昆明理工大学昆华医学院，昆明 650504；2 云南省第一人民医院干部保健科，昆明 650031；3 太原理工大学现代科技学院，太原 030021；4 沈阳康硒生物工程研究所，沈阳110015；5 中国农业大学农学与生物技术学院，北京 100094；6 昆明医科大学基础医学院，昆明 650504

出版日期:2014-07-31 发布日期:2014-11-09

Effect of Se-enriched Ganoderma lucidum on Acetyl Coenzyme A Carboxylase α Expression of Rats in the Treatment of Non-alcoholic Fatty Liver Disease

WU Jun-zi1,2, JIA Ya-min3, SHEN Ping-rui4, HU Yue-gao5, QUAN Sheng-lin2, LI Yan2*, LI Shu-de6*

1 Kunming University of Science and Technology,Collage of Kunhua Medicine,Kunming 650504, China; 2First People's Hospital of Yunnan Province,Cadres Health Care,Kunming 650031 ,China; 3 Polytechnic Institute Taiyuan University of Technology,Taiyuan 030021 ,China; 4 Kang Se Shenyang Institute of Biotechnology, Shenyang 110015, China; 5 China Agricultural University,College of Agronomy and Biotechnology,Beijing 100094 ,China; 6 Kunming Medical University, Collage of Basic Medical, Kunming 650504 ,China

Online:2014-07-31 Published:2014-11-09

摘要/Abstract

摘要： 60只SD大鼠随机分为正常对照组、模型对照组、富硒灵芝治疗组。非酒精性脂肪肝病（Non-Alcoholic Fatty Liver Disease，NAFLD）模型建立成功后富硒灵芝治疗组给予富硒灵芝水灌胃治疗，模型对照组给予生理盐水灌胃辅助对比，空白对照组不加以任何干涉，分别于6周和12周处死大鼠各半。大鼠肝脏HE染色显示，治疗6周后富硒灵芝组脂肪变性程度及细胞肿胀相较于模型组明显减轻，炎性细胞浸润及坏死灶较模型对照组明显减少，富硒灵芝12周组大鼠比富硒灵芝6周组肝脏改善效果更佳；治疗6周后，富硒灵芝治疗组与模型对照组相比，血清中乳酸脱氢酶（LDH）、天门冬氨酸氨基转移酶（AST）、丙氨酸氨基转移酶（ALT）、谷胱甘肽（GSH）、丙二醛（MDA），肝脏组织中超氧化物歧化酶（SOD）、MDA和GSH，差异显著（P<0.05）；SERBF-1mRNA和蛋白表达：富硒灵芝6周组与模型对照6周组相比，只有微小差异（P>0.05），12周富硒灵芝组与模型对照组相比，差距则具有统计学意义（P<0.05）；ACCα mRNA和蛋白表达：富硒灵芝6周组与模型对照6周组相比，差别具有统计学意义（P<0.05）；12周时差异更加显著；胰岛素抵抗指数6周时没有改善，12周时呈现出统计学意义（P<0.05）。因而推测，富硒灵芝可通过调控大鼠ACCα的表达、以提高抗氧化能力及改善肝功能来治疗NAFLD，但药效疗程较长，可能需长期服用。

关键词: 富硒灵芝, 非酒精性脂肪肝, 乙酰辅酶A羧化酶&alpha, 固醇调节元件结合转录因子1

Abstract: 60 SD rats were randomly divided into normal control group,model control group, Se-enriched Ganoderma lucidum treatment group.After non-alcoholic fatty liver disease (NAFLD) model was successfully established,the Se-enriched G.lucidum treatment group received Se-enriched G.lucidum water gavage treatment and the model control group received saline auxiliary for comparison while the control group were treated with no interference.After 6 weeks and 12 weeks,half of the rats were sacrificed each time.HE staining showed that,after 6 weeks’ treatment,the steatosis and cell swelling was significantly reduced in the rats with Se-enriched G.lucidum treatment.The infiltration and necrosis in inflammatory cell was significantly reduced compared with the model group.The effect of 12 weeks’ treatment with Se-enriched G.lucidum was better than that of 6 weeks’.After 6 weeks’ treatment,the level of lactate dehydrogenase (LDH),aspartate aminotransferase (AST), alanine aminotransferase (ALT),serum glutathione glutathione (GSH),malondialdehyde (MDA),liver superoxide dismutase (SOD),liver MDA and liver GSH in the group with Se-enriched G.lucidum treatment changed significantly,compared with the control group, P<0.05.Comparing the SERBF-1 mRNA and protein expression in Se-enriched G.lucidum 6 weeks group with the expression in control group,there was only minor differences (P> 0.05),but the difference was statistically significance (P<0.05) between 12 weeks group and control group.ACCα mRNA and protein expression:comparing the Se-enriched G.lucidum 6 weeks’ group with model control group,the difference was statistically significant (P<0.05) and the difference was more significant at the 12 weeks’ group.The 6 weeks’ insulin resistance index (IR),had no improvement,however,it was statistically significant (P<0.05) at the 12 weeks’ group.Thus it was speculated that Se-enriched G.lucidum had the effect of regulating the expression of rat ACCα to increase antioxidant capacity and to improve liver function to treat NAFLD,but the efficacy need a longer course of treatment,and it may take a long-term use.

Key words: Se-enriched Ganoderma lucidum, non-alcoholic fatty liver disease, acetyl-CoA carboxylase alpha；sterol regulatory element binding transcription factor 1

中图分类号:

R285

武俊紫1,2，贾亚敏3，沈平瑞4，胡跃高5，全胜麟2，李燕2*，李树德6*. 富硒灵芝调控大鼠乙酰辅酶A羧化酶α表达治疗非酒精性脂肪肝病的研究[J]. 天然产物研究与开发, 2014, 26(7): 1086-1092.

WU Jun-zi1,2, JIA Ya-min3, SHEN Ping-rui4, HU Yue-gao5, QUAN Sheng-lin2, LI Yan2*, LI Shu-de6*. Effect of Se-enriched Ganoderma lucidum on Acetyl Coenzyme A Carboxylase α Expression of Rats in the Treatment of Non-alcoholic Fatty Liver Disease[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2014, 26(7): 1086-1092.

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