天然产物研究与开发 ›› 2015, Vol. 27 ›› Issue (2): 294-300.doi: 10.16333/j.1001-6880.2015.02.021

• 开发研究 • 上一篇    下一篇

贵州野生甜藤多糖的提取与脱蛋白方法研究

常飞1,2*，王绍云1，陈飞1   

  1. 1 凯里学院化学与材料工程学院，凯里 556011；2 贵州大学精细化工研究中心，贵阳 550025
  • 出版日期:2015-02-15 发布日期:2015-02-15

Extraction and Deproteinization of Polysaccharides from Guizhou wild Paederia scandens

CHANG Fei1,2*, WANG Shao-yun1, CHEN Fei1   

  1. 1 College of Chemistry and Materials Engineering,Kaili University,Guizhou Kaili 556011,China; 2 Center for Research and Development of Fine Chemicals,Guizhou University,Guizhou Guiyang 550025,China
  • Online:2015-02-15 Published:2015-02-15

PDF (PC)

313

摘要： 以贵州野生甜藤干粉为原料，通过单因素试验，再通过正交试验优化甜藤多糖提取工艺，并采用Sevag法、TCA法、Sevag-TCA法、HClO4法进行脱蛋白方法研究。结果表明，甜藤多糖最优提取工艺为：以水为溶剂，料液比1:50，超声功率70 W，水浴温度70 ℃，超声提取时间110 min，提取2次；HClO4法脱蛋白效果最佳，经3次脱蛋白处理后蛋白脱出率为94.78%，多糖损失率为15.14%，该法用于脱除甜藤植物多糖中的蛋白质，工艺简单，成本低，具有一定应用价值。

关键词: 甜藤, 多糖, 提取, 脱蛋白

Abstract: A process for extraction of polysaccharides from Guizhou wild Paederia scandens powder was optimized through single factor test and orthogonal test.Four different methods,namely Sevag,TCA,Sevag-TCA,and HClO4 methods,for the removal of protein were investigated.The results showed that HClO4 method was demonstrated to be the best way for the removal of protein,and a protein emergence rate of 94.78% can be achieved after deproteinizing protein for 3 times.Moreover,15.14% polysaccharide loss rate was detected under the optimal extraction conditions of using water as solvent,solid-liquid ratio of 1:50,and ultrasonic power of 70 W at a bath temperature of 70 ℃ within an extraction time of 110 min repeating for two times.This process was simple and low cost.

Key words: Paederia scandens (Lour.) Meer., polysaccharide, extraction, deproteinization

中图分类号: 

O658.9

版权所有 © 《天然产物研究与开发》编辑部
地址：四川天府新区群贤南街289号　邮编：610299
电话：028-85210304　QQ群：342035423　Email：trcw@clas.ac.cn; re_trcw@clas.ac.cn　
本系统由北京玛格泰克科技发展有限公司设计开发
总访问量：　今日访问：　在线人数：