天然产物研究与开发 ›› 2015, Vol. 27 ›› Issue (6): 958-961.doi: 10.16333/j.1001-6880.2015.06.004

• 研究论文 • 上一篇    下一篇

狗脊化学成分及抑制乙酰胆碱酯酶生物活性研究

殷帅文1*,何旭梅2,王伟1,赖强1,马宁1,王安萍1   

  1. 1 井冈山大学生命科学学院;2 井冈山大学电信学院,吉安 343009
  • 出版日期:2015-06-30 发布日期:2015-07-10

Constituents and Acetylcholinesterase Inhibitory Activity of Woodwardia japonica

YIN Shuai-wen1*,HE Xu-mei2,WANG Wei1,LAI Qiang1,MA Ning1,WANG An-ping1   

  1. 1 School of Life Science,Jinggangshan University;2 School of Electronics and information engineering,Jinggangshan University,Ji’an 343009,China
  • Online:2015-06-30 Published:2015-07-10

摘要: 采用多种分离方法从狗脊(Woodwardia japonica)乙醇提取物中分离纯化出7个化合物,经现代波谱分析将它们的结构分别鉴定为3-O-[6’-O-(9-Z-二十碳酰)-β-D-葡萄糖酰]-谷甾醇(1)、β-谷甾醇-3-O-α-L-(6’-O-正十六酰基)-葡萄糖苷(2)、β-谷甾醇(3)、狗脊酸(4)、胡萝卜苷(5)、山柰素-3-O-α-L-鼠李糖基-7-O-α-L-鼠李糖苷(6)和山柰素-3-O-α-L-(4-O-乙酰基)-鼠李糖基-7-O-α-L-鼠李糖苷(7)。其中化合物1的结构还未见报道。乙酰胆碱酯酶抑制活性测试表明化合物4具有一定抑制乙酰胆碱酯酶活性。

关键词: 狗脊, 化学成分, 生物活性

Abstract: The chemical constitutes of the EtOH extract of Woodwardia japonica from Jiangxi Province were studied.Seven compounds were isolated by repeated gel column chromatography.On the basis of spectral data,their structures were identified as 3-O-[6’-O-(9-Z-eicosenoicenoyl)-β-D-glucopyranosyl]-sitosterol (1),β-sitosterol-3-o-α-L-(6’-O-hexadecanoyl) glucoside (2), β-sitosterol (3),woodwardinic acid (4),daucosterol (5),kaempferol-3-O-α-L-rhamnopyranoside7-O-α-L-rhamnopyranoside (6) and kaempferol-3-O-α-L-(4-O-acetyl)-rhamnopyranoside7-O-α-L-rhamnopyranoside (7).Compound 1 was a new compound and compound 4 showed acetylcholinesterase inhibitory activity.

Key words: Woodwardia japonica, chemical constituents, bioactivity

中图分类号: 

Q501 Q503