天然产物研究与开发 ›› 2015, Vol. 27 ›› Issue (6): 1070-1075.doi: 10.16333/j.1001-6880.2015.06.024

• 开发研究 • 上一篇    下一篇

油樟油对油樟内生真菌中活性化合物影响

谭韵雅1,卢红1,李群1*,魏琴2*   

  1. 1 四川师范大学生命科学学院,成都 610101;2 宜宾学院发酵资源与应用四川省高校重点实验室,宜宾 644000
  • 出版日期:2015-06-30 发布日期:2015-07-10

Effects of Cinnamomum longepaniculatum Oil on the Active Components of Endophytic Fungi

TAN Yun-ya1, LU Hong1, LI Qun1*, WEI Qin 2*   

  1. 1 College of Life Science,Chengdu 610101,China; 2 Yibin College Fermentation Resources Universities of Sichuan Key Laboratory,Yibin 644000,China
  • Online:2015-06-30 Published:2015-07-10

摘要: YY26、YG42和YG71是从油樟中分离得到的三株内生真菌。本研究采用GC-FID、GC-MS技术,以油樟油作为诱导物,研究YY26、YG42、YG71菌株在不同培养基条件下对菌株中活性化合物1,8-桉叶油素、松油烯-4-醇、a-松油醇、r-松油烯产量的影响。结果表明:在PDB培养基中,一方面,油樟油可促进YY26、YG42菌株积累1,8-桉叶油素等物质,其中对1,8-桉叶油素的促进最强,分别提高1.41倍和1.30倍;另一方面,油樟油抑制YG71菌株中以上4种物质的积累。在察氏培养基中,油樟油对YY26、YG42及YG71菌株中4种物质的积累没有明显的促进作用。该研究为植物有用代谢产物的规模化生产提供了理论依据,有较强的实践意义。

关键词: 油樟油, 内生真菌, GC-FID, GC-MS, 1, 8-桉叶油素

Abstract: YY26,YG42 and YG71 were three endophytic fungi strains isolated from Cinnamomum longepaniculatum. With C.longepaniculatum oil as inductor,GC-FID and GC-MS were used to analyze the accumulation of 1,8-cineole, terpinen-4-ol, a-terpineol and r-Terpinene in YY26,YG42,and YG71 strains under different media conditions. The experimental results indicated that in PDB medium C.longepaniculatum oil can promote accumulation of 1,8-cineole inYY26,YG42 strains and the content of 1,8-cineole increased by 1.41 and 1.30 times respectively,while 1,8-cineole were repressed in strains YG71 in PDB medium. C.longepaniculatum oil had no obvious promotion effect on the 4 compounds in czapek′s medium.This study provided scientific basis for the large-scale production of natural metabolites.

Key words: Cinnamomum longepaniculatum oil, endophytic fungi, GC-FID, GC-MS, 1,8-cineole

中图分类号: 

Q939.97