天然产物研究与开发 ›› 2020, Vol. 32 ›› Issue (11): 1811-1817.doi: 10.16333/j.1001-6880.2020.11.002

• 研究论文 • 上一篇    下一篇

黄精地上部位化学成分及其胰脂肪酶抑制活性研究

王亚凤，张祖湘，何瑞杰，阳丙媛，王磊，黄永林*


  

  1. 广西壮族自治区中国科学院广西植物研究所 广西植物功能物质研究与利用重点实验室，桂林 541006

  • 出版日期:2020-11-28 发布日期:2020-12-04
  • 基金资助:
    广西自然科学基金（2018GXNSFAA294033，2018GXNSF AA281078）；广西创新驱动发展专项（桂科AA18118015）

Study on the chemical constituents of the aerial parts of Polygonatum sibiricum and its pancreatic lipase inhibitory activity

WANG Ya-feng,ZHANG Zu-xiang,HE Rui-jie,YANG Bing-yuan,WANG Lei,HUANG Yong-lin*   

  1. Guangxi Key Laboratory of Functional Phytochemicals Research and Utilization,Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences,Guilin 541006,China

  • Online:2020-11-28 Published:2020-12-04

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摘要：

研究黄精（Polygonatum sibiricum）叶子中的化学成分及其对胰脂肪酶活性的抑制。主要运用Sephadex LH-20、Diaion HP20ss、Toyopearl HW-40F和ODS C18等柱色谱和中低压半制备液相对其乙醇提取物进行化学成分研究，采用胰脂肪酶抑制剂活性筛选模型对部分化合物进行活性筛选。实验结果共分离纯化、鉴定14个化合物，经NMR、高分辨率质谱等波谱学数据分析及与文献数据对比，化合物结构分别鉴定为咖啡酸甲酯（1）、绿原酸（2）、异牡荆素（3）、异荭草素（4）、荭草素（5）、荭草素 2″-O-木糖甙（6）、木犀草素6-C-β-D-吡喃半乳糖基（1→2）-β-D-吡喃葡萄糖苷（7）、芹菜素6-C-β-D-吡喃半乳糖基（1→2）-β-D-吡喃葡萄糖苷（8）、芹菜素-6-C-β-D-吡喃木糖基（1→2）-β-D-吡喃葡萄糖苷（9）、 荭草素-6-C-β-D-葡萄糖苷 (10)、异牡荆素8-C-β-D-葡萄糖苷（11）、金圣草黄素（12）、槲皮素3-O-α-L-鼠李糖苷（13）、正二十一烷酸（14）。其中，化合物6~14为首次从该植物中分离得到。共筛选了化合物2、3、6、7、8、10、11、13的胰脂肪酶活性，结果显示该8个化合物对胰脂肪酶均具有一定的抑制活性。

关键词: 黄精, 化学成分, 结构鉴定, 碳苷黄酮, 胰脂肪酶抑制剂

Abstract:

To study the chemical constituents of the aerial parts parts of Polygonatum sibiricum and its pancreatic lipase inhibitory activity.Compounds were isolated from the ethanol extract of the aerial parts of P. sibiricum by various chromatographic techniques including Sephadex LH-20,Diaion HP20ss,Toyopearl HW-40F,ODS C18 and medium-low pressure semi-preparative liquid phase.The inhibitor activity was detected by porcine pancreatic lipase inhibitor activity screening model.As a result,a total of 14 compounds were isolated and purified.Their structures were elucidated on the basis of NMR,MS spectroscopic and comparison with literature data as methyl caffeate (1),chlorogenic acid (2),isovitexin (3),isoorientin (4),orientin (5),orientin 2″-O-xyloside (6),luteolin 6-C-β-D-galactosyl (1→2)-β-D-glucoside (7),apigenin 6-C-β-D-galactosyl (1→2)-β-D-glucoside (8),luteolin 6-C-β-D-xylosyl (1→2)-β-D-glucoside (9),orientin-6-C-β-D-glucoside (10),isovitexin 8-C-β-D-glucoside (11),chrysoeriol (12),quercetin 3-O-α-L-rhamnoside (13),n-heneicosanoic acid (14).Among them,compounds 6-14 were reported from P. sibiricum for the first time.Compounds 2,3,6,7,8,10,11,13 were screened for pancreatic lipase inhibitory activity,and the results showed that the eight compounds have certain inhibitory activity on pancrelipase.

Key words: Polygonatum sibiricum, chemical composition, structural identification, carboflavonoid, pancreatic lipase inhibitor

中图分类号:  O629.9

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