天然产物研究与开发 ›› 2020, Vol. 32 ›› Issue (12): 2051-2060.doi: 10.16333/j.1001-6880.2020.12.009

• 研究简报 • 上一篇    下一篇

HPLC指纹图谱结合多指标含量测定的八角莲质量评价研究

崔誉文1*，何昊1，张丽2，李玉泽2，黄文丽2，张化为2，宋小妹2


  

  1. 1西安医学院 药学院，西安 710021；2陕西中医药大学 陕西省秦岭中草药应用开发工程技术研究中心/陕西省中药基础与新药研究重点实验室，咸阳712046

  • 出版日期:2020-12-28 发布日期:2020-12-24
  • 基金资助:
    陕西省重点研发计划（2019ZDLSF04-03-02)；陕西中医药大学学科创新团队项目（2019-YL12）；陕西省高校青年杰出人才支持计划配套科研项目（05041904）

Study on quality evaluation of Dysosma versipellis by HPLC fingerprint combined with multi-index content determination

CUI Yu-wen1*,HE Hao1,ZHANG Li2,LI Yu-ze2,HUNAG Wen-li2,ZHANG Hua-wei2,SONG Xiao-mei2   

  1. 1School of Pharmacy,Xi’an Medical University,Xi’an 710021,China;2 School of Pharmacy,Shaanxi University of Chinese Medicine,Shaanxi Qinling Chinese Herbal Medicine Application Development Engineering Technology Research Center/Shaanxi Provincial Key Laboratory of Traditional Chinese Medicine and New Drug Research,Xianyang 712046,China

  • Online:2020-12-28 Published:2020-12-24

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摘要：

建立八角莲HPLC指纹图谱，并同时测定5种化学成分的含量，为其质量评价提供依据。采用Thermo HyPURITY C18色谱柱（250 mm×4.6 mm,5 μm），以甲醇（A）-0.4%磷酸水溶液（B）为流动相，梯度洗脱，柱温30 ℃，体积流量1.0 mL/min，检测波长290 nm，进样量10 μL，建立了八角莲的HPLC指纹图谱。16批样品指纹图谱中有28个共有峰，相似度较好，经与对照品比照确认了8个成分，对(+)-tanegool-7′-methylether、4′-去甲基鬼臼毒素、槲皮素、鬼臼毒素、山柰酚5种化学成分进行了定量分析，5个化学成分在各自范围内线性关系良好（r2≥0.999 0），精密度、重复性、稳定性均符合定量要求；加样回收率在98.7%~99.6%，RSD小于3%。所建立的方法简便、稳定、可靠，HPLC指纹图谱结合多指标成分含量测定可用于八角莲药材质量的评价。

关键词: 八角莲, HPLC, 指纹图谱, 聚类分析, 含量测定

Abstract:

To provide the scientific basis for the quality evaluation of Dysosma versipellis, its HPLC fingerprints was established,and the contents of five components were simultaneously determined.The analyses were performed on Thermo HyPURITY C18 column (250 mm×4.6 mm,5 μm),and the mobile phase comprised of MeOH (A) - 0.4% phosphoric acid aqueous solution (B) with the gradient elution at a flow rate of 1.0 mL/min.The detection wavelength was set at 290 nm;the column temperature was maintained at 30 ℃ and the injection volume was 10 μL.The HPLC fingerprint with twenty-eight common peaks of D. versipellis in 16 batches of sample was established,and the similarities of samples were good.Eight components were identified by comparison with the reference substances.Five components,namely,(+)-tanegool-7′-methylether,4′-demethylpodophyllotoxin,quercetin,podophyllotoxin,kaempferol were analyzed quantitatively.The linear relationship of five components was good (r2≥0.999 0).The precision,repeatability and stability were all in line with quantitative requirements.The average recoveries (n= 6) of five components were between 98.7% and 99.6%,and the RSD were less than 3%.The established method is simple,stable and reliable,and can be expected for the quality control of D. versipellis.

Key words: Dysosma versipellis, HPLC, fingerprints, cluster analysis, content determination

中图分类号:  R282.5

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