天然产物研究与开发 ›› 2022, Vol. 34 ›› Issue (1): 1-6.doi: 10.16333/j.1001-6880.2022.1.001

• 研究论文 •    下一篇

中药灵芝降羊毛甾烷三萜类成分研究

梁旭博1,2，王妍1,2，司盈盈1,2，赵琪璐1，赵晨光1,2，杜锟1,2，冯卫生1,2*，赵珍珠1,2*   

  1. 1河南中医药大学药学院；2河南省中药开发工程技术研究中心，郑州 450046
  • 出版日期:2022-01-28 发布日期:2022-01-28
  • 基金资助:
    国家自然科学基金（82003607）；河南中医药大学科研苗圃工程项目（MP2020-24）；河南省中医药科学研究专项（20-21ZY1039）；河南省高等学校重点科研项目（21A360002）

Study on lanostane-type nortriterpenoids from Ganoderma lingzhi

LIANG Xu-bo1,2,WANG Yan1,2,SI Ying-ying1,2,ZHAO Qi-lu1,ZHAO Chen-guang1,2,DU Kun1,2,FENG Wei-sheng1,2*,ZHAO Zhen-zhu1,2*   

  1. 1School of Pharmacy,Henan University of Chinese Medicine;2The Engineering and Technology Center for Chinese Medicine Development of Henan Province,Zhengzhou 450046,China
  • Online:2022-01-28 Published:2022-01-28

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摘要： 研究灵芝Ganoderma lingzhi子实体的化学成分。采用正相硅胶、ODS、Sephadex LH-20凝胶柱色谱和prep-HPLC等方法分离与纯化，运用NMR、MS等波谱技术鉴定化合物结构。从灵芝95%乙醇提取物中分离得到5个降羊毛甾烷三萜类化合物，分别是ethyl 20(21)-dehydrolucidenate A（1）、lingzhi-20(21)-en-24-oic acid A（2）、20(21)-dehydrolucidenic acid A（3）、赤芝酮A（4）、lucidadone H（5）。化合物1和2是两个新的降羊毛甾烷三萜化合物；化合物3~5为首次从该属真菌中分离得到。化合物1和2在白血病（HL-60）、肺癌（A549）、肝癌（SMMC-7721）、乳腺癌（MCF-7）、结肠癌（SW480）五种不同的癌细胞株上进行细胞毒活性筛选，结果显示化合物1~5在40 μM时无明显的细胞毒活性。


关键词: 灵芝, 化学成分, 降羊毛甾烷三萜

Abstract:

To study the chemical constituents from the fruiting bodies of Ganoderma lingzhi,all compounds were isolated and purified by various column chromatographies,including silica gel,ODS,Sephadex LH-20 and prep-HPLC,and their structures were identified by NMR,MS and other spectroscopic techniques.Five lanostane-type nortriterpenoids were isolated from 95% ethanol extract of G. lingzhi,which were identified as ethyl 20(21)-dehydrolucidenate A (1),lingzhi-20(21)-en-24-oic acid A (2),20(21)-dehydrolucidenic acid A (3),lucidone A (4),and lucidadone H (5).Compounds 1 and 2 have not been described before,and compounds 3-5 are isolated from this fungus for the first time.Cytotoxic effects of compounds 1-5 were investigated against five regular cancer cell lines,and the results showed that none of them showed significant activity.

Key words: Ganoderma lingzhi, chemical constituent, lanostane-type nortriterpenoids

中图分类号:  R284.1

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