天然产物研究与开发 ›› 2022, Vol. 34 ›› Issue (10): 1713-1717.doi: 10.16333/j.1001-6880.2022.10.009

• 研究简报 • 上一篇    下一篇

菟丝子中一个新的吲哚类生物碱

杜   锟1,2*,薛贵民1,支燕乐1,赵珍珠1,司盈盈1,陈   辉1,马金莲3*   

  1. 1河南中医药大学药学院,郑州 450046;2河南中医药大学第一附属医院,郑州 450000;3河南中医药大学中医药科学院,郑州 450046
  • 出版日期:2022-10-28 发布日期:2022-10-31
  • 基金资助:
    河南省科技攻关(182102310288,212102310314);河南中医药大学博士科研基金(BSJJ2018-19,00104311-2019-7)

A new indole alkaloid from Cuscutae Semen

DU Kun1,2*,XUE Gui-min1,ZHI Yan-le1,ZHO Zhen-zhu1,SI Ying-ying1,CHEN Hui1,MA Jin-lian3*   

  1. 1School of Pharmacy,Henan University of Chinese Medicine,Zhengzhou 450046,China; 2The First Affiliated Hospital of Henan University of Chinese Medicine,Zhengzhou 450000,China;3Academy of Chinese Medical Sciences,Henan University of Chinese Medicine,Zhengzhou,Henan 450046,China
  • Online:2022-10-28 Published:2022-10-31

摘要:

研究中药菟丝子(Cuscutae Semen)的化学成分。采用Sephadex LH-20和硅胶等柱色谱以及反相制备型HPLC等方法分离与纯化,运用NMR、MS等波谱技术鉴定化合物结构。从菟丝子的甲醇提取物中分离得到6个化合物,分别鉴定为菟丝子新碱A(1)、金丝桃苷(2)、槲皮素-3-O-β-D-半乳糖-(2→1)-β-D-芹糖苷(3)、槲皮素(4)、山奈酚(5)、芝麻素(6)。其中化合物1为一个新的β-卡波林类生物碱。化合物25对组蛋白赖氨酸特异性去甲基化酶1(lysine specific demethylase 1,LSD1)显示出较强的抑制作用,IC50分别为5.74 ± 0.49和3.94 ± 0.27 μmol/L。

关键词: 菟丝子, 化学成分, 吲哚类生物碱, 黄酮苷, LSD1抑制活性

Abstract:

To study the chemical constituents from Cuscutae Semen.The compounds were isolated and purified by various modern chromatographic techniques,including column chromatography (Sephadex LH-20 or silica gel) and preparative HPLC,and their structures were identified by spectroscopic data and physicochemical properties.Six compounds were isolated from the MeOH extract of Cuscutae Semen,which were elucidated as cuscutalin A (1),hyperoside (2),quercetin 3-O-β-D-apiofuranosyl-(1 →2)-β-D-galactoside (3),quercetin (4),kaempferol (5),and sesamin (6).Compound 1 is a new β-carboline alkaloid.Compounds 2 and 5 exhibited strong inhibitory activity against lysine specific demethylase 1 with the IC50 values of 5.74±0.49 and 3.94±0.27 μmol/L,respectively.

Key words: Cuscutae Semen, chemical constituents, indole alkaloid, flavonol glycoside, LSD1 inhibitory activity

中图分类号:  R284.1