天然产物研究与开发 ›› 2022, Vol. 34 ›› Issue (12): 2077-2088.doi: 10.16333/j.1001-6880.2022.12.012

• 开发研究 • 上一篇    下一篇

四川小金县野生羊肚菌多糖的结构解析及体外抗肿瘤活性初探

周丽倩1,黄   瑶1,鲁艳1,叶姿妤1,刘欣岚2,丁   祥2,侯怡铃1*   

  1. 1西华师范大学生命科学学院 西南野生动植物资源保护教育部重点实验室;2西华师范大学环境科学与工程学院,南充 637009
  • 出版日期:2022-12-28 发布日期:2022-12-29
  • 基金资助:
    四川省科技厅应用基础重点项目(2018JY0087);四川省科技厅重点研发项目(2018NZ0055)

Structural identificatin and in vitro antitumor activity of wild Morchella esculenta polysaccharide from Xiaojin County of Sichuan Province

ZHOU Li-qian1,HUANG Yao1,LU Yan1,YE Zi-yu1,LIU Xin-lan2,DING Xiang2,HOU Yi-ling1*   

  1. 1Key Laboratory of Southwest China Wildlife Resources Conservation (Ministry of Education),College of Life Sciences,China West Normal University; 2College of Environmental Science and Engineering,China West Normal University,Nanchong 637009,China
  • Online:2022-12-28 Published:2022-12-29

摘要:

本研究通过热水浸提法从四川小金县的野生羊肚菌中提取多糖(Morchella esculenta polysaccharide,ME-P),利用傅里叶红外光谱(FT-IR)、高效液相色谱(HPLC)、气相质谱连用(GC-MS)和核磁共振(NMR)波谱等技术鉴定ME-P的结构,采用CCK-8法检验ME-P的药物毒性并探寻ME-P体外抗肿瘤活性。结果表明,ME-P中含有吡喃糖环,单糖组成为甘露糖、葡萄糖、半乳糖,比例为4∶4∶1;具有1,2,3,4,6-D-甘露糖、2,3,4,6-D-甘露糖交替连接为主链,1,6-D-葡萄糖和1,2,6-D-半乳糖作为支链,4-D-葡萄糖与1,2,6-D-半乳糖连接作为末端糖的重复单位的结构新颖的多糖。ME-P对巨噬细胞(RAW 264.7)无毒害,且有极显著(D<0.01)增殖作用,在质量浓度为1.25 μg/mL时对RAW 264.7的增殖率可高达79.5%。同时,对小鼠结肠癌细胞(CT26.WT)和胃癌细胞(MFC)均有抑制作用,且均为低浓度时抑制效果较好,在ME-P为1.25 μg/mL时,ME-P对CT26.WT和MFC的抑制率分别为28.20%和34.23%。本研究解析出四川小金县羊肚菌多糖(ME-P)为甘露糖、葡萄糖、半乳糖组成的结构新颖的多糖,无毒且对肿瘤细胞显示出显著的抑制作用,该结果为羊肚菌多糖的开发与应用提供了一定的科学依据。

关键词:

Abstract:

In this study,Morchella esculenta polysaccharide(ME-P) were extracted by hot water extraction from wild M. esculenta in Xiaojin County,Sichuan Province.Fourier transform infrared spectroscopy (FT-IR),high performance liquid chromatography (HPLC),gas chromatography mass spectrometry (GC-MS) and nuclear magnetic resonance (NMR) were used to identify the structure of ME-P.CCK-8 method was used to detect the drug toxicity of ME-P and to explore the antitumor activity of ME-P in vitro.The results showed that ME-P contained pyranose ring,and the monosaccharides were composed of mannose,glucose and galactose with a ratio of 4∶4∶1.The polysaccharides are characterized by a novel structure,which is characterized by the alternating linkage of 1,2,3,4,6-D-mannose and 2,3,4,6-D-mannose as the main chain,1,6-D-glucose and 1,2,6-D-galactose as the branch chain,and the connection of 4-D-glucose and 1,2,6-D-galactose as a repeating unit of terminal monosaccharide.ME-P is non-toxic to macrophages (RAW 264.7) and has a very significant effect (P<0.01) on proliferation.The proliferation rate of RAW 264.7 can be as high as 79.5% at a mass concentration of 1.25 μg/mL.At the same time,it had inhibitory effect on mouse colon cancer cells (CT26.WT) and gastric cancer cells (MFC),and the inhibition effect was better at low concentration.When ME-P was 1.25 μg/mL,the inhibition rates of ME-P on CT26.WT and MFC were 28.20% and 34.23%,respectively.This study shows that M. esculenta polysaccharides (ME-P) in Xiaojin County,Sichuan Province is a novel polysaccharide composed of mannose,glucose and galactose,which is non-toxic and has significant inhibitory effect on tumor cells.The results provide a scientific basis for the development and application of Morchella polysaccharides.

Key words: Morchella esculenta, polysaccharides, structure analysis, in vitro, antitumor activity 

中图分类号:  R93