天然产物研究与开发 ›› 2020, Vol. 32 ›› Issue (9): 1592-1598.doi: 10.16333/j.1001-6880.2020.9.018

• 开发研究 • 上一篇    下一篇

苦豆碱对缺氧/复氧损伤的人脐静脉内皮细胞保护机制研究

马会军*，朱米娜，杜燕   

  1. 西安市第一医院心血管内科，西安 710002

  • 出版日期:2020-09-28 发布日期:2020-09-25
  • 基金资助:
    西安市第一医院心血管内科科室基金（ZDXA2018016）

Protective mechanism of aloperine on hypoxia/reoxygenation injured human umbilical vein endothelial cells

MA Hui-jun*,ZHU Mi-na,DU Yan #br#

#br#
  

  1. Department of Vasculocardiology,Xi’an No.1 Hospital,Shaanxi 710002,China

  • Online:2020-09-28 Published:2020-09-25

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摘要： 苦豆碱（aloperine，ALO）是一种具有抗炎、抗肿瘤及抗感染功效的生物碱，但其对人脐静脉内皮细胞缺氧/复氧损伤的影响尚不明确。本研究利用人脐静脉内皮细胞建立了体外缺氧/复氧损伤的细胞模型，对细胞进行分组处理：对照组、缺氧/复氧损伤组、苦豆碱（20、50和100 μmol/L）预处理组。对细胞活力、细胞内乳酸盐脱氢酶（LDH）、丙二醛（MDA）和超氧化物歧化酶（SOD）活性，细胞内白细胞介素-1β（IL-1β）和肿瘤坏死因子α（TNF-α），以及内质网应激相关蛋白GRP78，XBP-1和凋亡相关蛋白CHOP的表达进行了检测。我们发现与缺氧/复氧损伤组相比，苦豆碱预处理能显著提高细胞活力和SOD活性、降低LDH活性、MDA含量以及IL-1β和TNF-α的水平（P<0.05）。另外，苦豆碱预处理还显著下调缺氧/复氧损伤引起的GRP78、XBP-1和CHOP水平的上升（P<0.05）。本研究证实苦豆碱能够提高细胞抗脂质过氧化反应的能力、降低炎症因子水平、抑制内质网应激引起的细胞凋亡，改善缺氧/复氧损伤引起的内皮细胞损伤。


关键词: 苦豆碱, 人脐静脉内皮细胞, 缺氧/复氧, 炎症, 内质网应激

Abstract:

Aloperine (ALO) is an alkaloid with anti-inflammatory,anti-tumor and anti-infection activities. However，its protective effects on hypoxia/reoxygenation (H/R) injured human umbilical vein endothelial cells (HUVECs) and involved mechanisms are unclear. In this study,hypoxia/reoxygenation injured HUVECs were constructed in vitro. The cells were divided into control group,hypoxia/reoxygenation group (H/R group) and different concentrations of aloperine (20,50 and 100 μmol/L) pretreated groups. Cell viability,the activity of lactate dehydrogenase (LDH) and superoxide dismutase (SOD),the content of methane dicarboxylic aldehyde (MDA),the level of intreleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α),and the protein expression of GRP78,XBP-1 and CHOP of different groups were measured. The results showed that compared with hypoxia/reoxygenation group,aloperine pretreatment could increase HUVECs viability and SOD,decreased LDH activity,MDA content,as well as levels of TNF-α and IL-1β (P<0.05). Aloperine pretreatment also down-regulated hypoxia/reoxygenation induced increase of GRP78,XBP-1 and CHOP (P<0.05). In summary,aloperine could improve the resistant of HUVECs against lipid peroxidation,decrease the levels of inflammatory cytokines and inhibit ERS related cell apoptosis,thus protect the HUVECs from hypoxia/reoxygenation injury.

Key words: aloperine, human umbilical vein endothelial cells, hypoxia/reoxygenation, inflammation, endoplasmic reticulum stress

中图分类号:  R54 R285

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