天然产物研究与开发 ›› 2022, Vol. 34 ›› Issue (11): 1911-1918.doi: 10.16333/j.1001-6880.2022.11.012

• 开发研究 • 上一篇    下一篇

生地黄水提物对大鼠睡眠的影响及其机制研究

李尧善1,2，申   旻3，王   盼1,2*   

  1. 1西北大学化工学院；2西北大学生物医药研究院，西安 710069；3陕西省太白酒业有限责任公司，宝鸡 722300
  • 出版日期:2022-11-25 发布日期:2022-11-25
  • 基金资助:
    国家自然科学基金（22108229）；陕西省教育厅专项科研计划（20JK0938）

Study on the effects and mechanism of Rehmanniae Radix water extract on sleep in rats 

LI Yao-shan1,2,SHEN Min3,WANG Pan1,2*   

  1. 1School of Chemical Engineering,Northwest University;2Biotech.& Biomed.Research Institute, Northwest University,Xi’an 710069,China;3Shaanxi Taibai Liquor Industry Co.,Ltd.,Baoji 722300,China
  • Online:2022-11-25 Published:2022-11-25

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摘要：

本实验旨在研究生地黄水提物对大鼠睡眠的影响，并探讨其可能的作用机制。参照《保健食品功能检验与评价方法》（2022版）中有助于改善睡眠的检验方法初步评价生地黄水提物的效果。进一步建立失眠大鼠模型，检测各给药组大鼠下丘脑中枢神经递质及相应受体的mRNA表达水平，并检测大鼠血清中炎症因子含量。结果显示生地黄水提物能改善大鼠睡眠且安全性良好，并且能显著增加失眠大鼠下丘脑中γ-氨基丁酸（gamma-aminobutyric acid，GABA）和5-羟色胺（5-hydroxytryptamine，5-HT）含量，显著降低谷氨酸（glutamic acid，Glu）和多巴胺（dopamine，DA）含量，相应神经递质的受体mRNA表达量也发生同样的变化趋势，此外给药组大鼠血清中细胞炎症因子TNF-α、IL-1β含量显著下降，IL-10的含量显著增加。说明生地黄水提物可通过调节神经递质和细胞炎症因子改善大鼠睡眠。

关键词: 生地黄, 改善睡眠, 氯苯丙氨酸, 神经递质, 细胞炎症因子

Abstract:

The purpose of this experiment is to study the effect of Rehmanniae Radix water extract on sleep in rats and explore its possible mechanism.The effect of Rehmanniae Radix water extract was evaluated by referring to the Functional Test and Evaluation Methods of Health Food (2022 Edition) for sleep improvement tests.Furthermore,the insomnia rat model was established,and the central neurotransmitters in the hypothalamus and their corresponding receptors mRNA expression levels were detected,as well as the content of inflammatory factors in the serum of the rats.The results showed that the Rehmanniae Radix water extract could improve the sleep of rats,and the safety was good.Furthermore,it significantly increased the contents of gamma-aminobutyric acid (GABA) and 5-hydroxytryptamine (5-HT) and decreased the contents of glutamic acid (Glu) and dopamine (DA) in the hypothalamus of insomniac rats,and the expression of receptor mRNA of the corresponding neurotransmitters was affected similarly.In addition,the contents of TNF-α and IL-1β in serum decreased significantly and IL-10 increased significantly.These data indicated that the Rehmanniae Radix water extract could improve sleep in rats by regulating neurotransmitters and cellular inflammatory factors.

Key words: Rehmanniae Radix, improving sleep, chlorophenylalanine, neurotransmitter, cellular inflammatory factor

中图分类号:  R285.5

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